pLV2-CMV-TTR-EGFP-mCherry plas

pLV2-CMV-TTR-EGFP-mCherry plasmid

PVT94508

pLV2-CMV-TTR-EGFP-mCherry plasmid Information

Plasmid name: pLV2-CMV-TTR(human)-EGFP-mCherry
Alias: NM_000371.4;ATTR; CTS; CTS1; HEL111; HsT2651; PALB;TBPA; TTN
Gene lenght: 441bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green,Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pIRES2-EGFP.P3'
Copy number: High
Induction:

pLV2-CMV-TTR-EGFP-mCherry plasmid Description

pLV2-CMV-TTR-EGFP-mCherry is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-TTR-EGFP-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggct 2821 tctcatcgtc tgctcctcct ctgccttgct ggactggtat ttgtgtctga ggctggccct 2881 acgggcaccg gtgaatccaa gtgtcctctg atggtcaaag ttctagatgc tgtccgaggc 2941 agtcctgcca tcaatgtggc cgtgcatgtg ttcagaaagg ctgctgatga cacctgggag 3001 ccatttgcct ctgggaaaac cagtgagtct ggagagctgc atgggctcac aactgaggag 3061 gaatttgtag aagggatata caaagtggaa atagacacca aatcttactg gaaggcactt 3121 ggcatctccc cattccatga gcatgcagag gtggtattca cagccaacga ctccggcccc 3181 cgccgctaca ccattgccgc cctgctgagc ccctactcct attccaccac ggctgtcgtc 3241 accaatccca aggaaggcgg tggaggcagt ggtggaggcg gtagcatggt gagcaagggc 3301 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 3361 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 3421 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 3481 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 3541 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 3601 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 3661 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 3721 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 3781 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 3841 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 3901 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 3961 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggatc ccgcccctct 4021 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 4081 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 4141 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 4201 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 4261 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 4321 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 4381 agttggatag ttgtggaaag agtcaaatgg ctcacctcaa gcgtattcaa caaggggctg 4441 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 4501 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg 4561 ttttcctttg aaaaacacga tgataatatg gtgagcaagg gcgaggagga taacatggcc 4621 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 4681 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 4741 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 4801 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 4861 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 4921 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 4981 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 5041 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 5101 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 5161 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 5221 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 5281 gacgagctgt acaagtgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga 5341 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 5401 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 5461 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 5521 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 5581 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 5641 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 5701 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 5761 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 5821 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 5881 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 5941 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 6001 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 6061 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 6121 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 6181 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 6241 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 6301 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 6361 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 6421 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 6481 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 6541 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 6601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6721 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6781 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac 6841 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 6901 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6961 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7021 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7081 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 7741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9001 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt 9061 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 9121 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 9181 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 9241 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 9301 aaagaaattg tatttgttaa atatgtacta caaacttagt agt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.