pLV3-CMV-FLAG-GPR61-CopGFP-Pur

pLV3-CMV-FLAG-GPR61-CopGFP-Puro plasmid

PVT96309

pLV3-CMV-FLAG-GPR61-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FLAG-GPR61(human)-CopGFP-Puro
Alias: NM_031936.6BALGR; GPCR3
Gene lenght: 1356bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FLAG-GPR61-CopGFP-Puro plasmid Description

pLV3-CMV-FLAG-GPR61-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FLAG-GPR61-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgG ACTACAAGGA CGACGATGAC 2341 AAGatggagt cctcacccat cccccagtca tcagggaact cttccacttt ggggagggtc 2401 cctcaaaccc caggtccctc tactgccagt ggggtcccgg aggtggggct acgggatgtt 2461 gcttcggaat ctgtggccct cttcttcatg ctcctgctgg acttgactgc tgtggctggc 2521 aatgccgctg tgatggccgt gatcgccaag acgcctgccc tccgaaaatt tgtcttcgtc 2581 ttccacctct gcctggtgga cctgctggct gccctgaccc tcatgcccct ggccatgctc 2641 tccagctctg ccctctttga ccacgccctc tttggggagg tggcctgccg cctctacttg 2701 tttctgagcg tgtgctttgt cagcctggcc atcctctcgg tgtcagccat caatgtggag 2761 cgctactatt acgtagtcca ccccatgcgc tacgaggtgc gcatgacgct ggggctggtg 2821 gcctctgtgc tggtgggtgt gtgggtgaag gccttggcca tggcttctgt gccagtgttg 2881 ggaagggtct cctgggagga aggagctccc agtgtccccc caggctgttc actccagtgg 2941 agccacagtg cctactgcca gctttttgtg gtggtctttg ctgtccttta ctttctgttg 3001 cccctgctcc tcatacttgt ggtctactgc agcatgttcc gagtggcccg cgtggctgcc 3061 atgcagcacg ggccgctgcc cacgtggatg gagacacccc ggcaacgctc cgaatctctc 3121 agcagccgct ccacgatggt caccagctcg ggggcccccc agaccacccc acaccggacg 3181 tttgggggag ggaaagcagc agtggttctc ctggctgtgg ggggacagtt cctgctctgt 3241 tggttgccct acttctcttt ccacctctat gttgccctga gtgctcagcc catttcaact 3301 gggcaggtgg agagtgtggt cacctggatt ggctactttt gcttcacttc caaccctttc 3361 ttctatggat gtctcaaccg gcagatccgg ggggagctca gcaagcagtt tgtctgcttc 3421 ttcaagccag ctccagagga ggagctgagg ctgcctagcc gggagggctc cattgaggag 3481 aacttcctgc agttccttca ggggactggc tgtccttctg agtcctgggt ttcccgaccc 3541 ctacccagcc ccaagcagga gccacctgct gttgactttc gaatcccagg ccagatagct 3601 gaggagacct ctgagttcct ggagcagcaa ctcaccagcg acatcatcat gtcagacagc 3661 tacctccgtc ctgccgcctc accccggctg gagtcatagg atccCTGCGA TATCCAGCgc 3721 ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 3781 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 3841 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 3901 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 3961 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 4021 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 4081 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 4141 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 4201 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 4261 gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg cccgccatgg 4321 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 4381 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc aaaggcgccc 4441 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 4501 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 4561 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 4621 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 4681 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 4741 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 4801 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 4861 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 4921 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 4981 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 5041 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga 5101 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 5161 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 5221 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 5281 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 5341 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 5401 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 5461 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 5521 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 5581 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 5641 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc 5701 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5761 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5821 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5881 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5941 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 6001 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6061 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 6121 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6181 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc 6241 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac 6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 6361 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 6421 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 6481 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 6541 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 6601 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 6661 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 6721 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 6781 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 6841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 6901 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 6961 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7081 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7141 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7561 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7621 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7681 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7741 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7801 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7921 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7981 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8041 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8101 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8161 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8221 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8281 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8341 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8401 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8461 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8521 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8581 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8641 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8701 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8761 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8821 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8881 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8941 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9181 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9241 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9301 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9361 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9421 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9481 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9541 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9601 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.