pLV3-CMV-GATA1-3×FLAG-CopGFP-P

pLV3-CMV-GATA1-3×FLAG-CopGFP-Puro plasmid

PVT94134

pLV3-CMV-GATA1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GATA1(human)-3×FLAG-CopGFP-Puro
Alias: NM_002049.4;CNSHA9; ERYF1; GATA-1; GF-1; GF1; HAEADA;NF-E1; NFE1; XLANP; XLTDA; XLTT
Gene lenght: 1239bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GATA1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GATA1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-GATA1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agttccctgg cctggggtcc 2341 ctggggacct cagagcccct cccccagttt gtggatcctg ctctggtgtc ctccacacca 2401 gaatcagggg ttttcttccc ctctgggcct gagggcttgg atgcagcagc ttcctccact 2461 gccccgagca cagccaccgc tgcagctgcg gcactggcct actacaggga cgctgaggcc 2521 tacagacact ccccagtctt tcaggtgtac ccattgctca actgtatgga ggggatccca 2581 gggggctcac catatgccgg ctgggcctac ggcaagacgg ggctctaccc tgcctcaact 2641 gtgtgtccca cccgcgagga ctctcctccc caggccgtgg aagatctgga tggaaaaggc 2701 agcaccagct tcctggagac tttgaagaca gagcggctga gcccagacct cctgaccctg 2761 ggacctgcac tgccttcatc actccctgtc cccaatagtg cttatggggg ccctgacttt 2821 tccagtacct tcttttctcc caccgggagc cccctcaatt cagcagccta ttcctctccc 2881 aagcttcgtg gaactctccc cctgcctccc tgtgaggcca gggagtgtgt gaactgcgga 2941 gcaacagcca ctccactgtg gcggagggac aggacaggcc actacctatg caacgcctgc 3001 ggcctctatc acaagatgaa tgggcagaac aggcccctca tccggcccaa gaagcgcctg 3061 attgtcagta aacgggcagg tactcagtgc accaactgcc agacgaccac cacgacactg 3121 tggcggagaa atgccagtgg ggatcccgtg tgcaatgcct gcggcctcta ctacaagcta 3181 caccaggtga accggccact gaccatgcgg aaggatggta ttcagactcg aaaccgcaag 3241 gcatctggaa aagggaaaaa gaaacggggc tccagtctgg gaggcacagg agcagccgaa 3301 ggaccagctg gtggctttat ggtggtggct gggggcagcg gtagcgggaa ttgtggggag 3361 gtggcttcag gcctgacact gggcccccca ggtactgccc atctctacca aggcctgggc 3421 cctgtggtgc tgtcagggcc tgttagccac ctcatgcctt tccctggacc cctactgggc 3481 tcacccacgg gctccttccc cacaggcccc atgcccccca ccaccagcac tactgtggtg 3541 gctccgctca gctcaggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3601 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3661 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3721 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3781 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3841 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3901 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3961 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4021 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4081 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4141 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4201 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4261 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4321 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4381 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4441 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4501 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4561 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4621 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4681 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4741 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4801 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4861 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4921 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4981 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5041 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5101 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5161 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5221 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5281 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5341 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5401 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5461 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5521 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5581 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5641 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5701 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5761 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5821 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5881 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5941 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6001 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6061 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6121 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6181 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6241 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6301 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6361 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6421 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6481 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6541 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6601 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6661 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 6721 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6781 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6841 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 6901 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6961 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7021 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7081 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7501 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7741 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8041 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8101 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8161 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8221 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8281 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8341 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8401 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8461 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8521 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8581 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8641 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8701 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8761 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8821 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8881 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8941 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9001 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9061 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9121 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9181 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9241 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9301 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9361 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9421 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9481 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.