pCMV-Ccdc191-EGFP-Neo plasmid

pCMV-Ccdc191-EGFP-Neo plasmid

PVT94226

pCMV-Ccdc191-EGFP-Neo plasmid Information

Plasmid name: pCMV-Ccdc191(mouse-toxic-opt)-EGFP-Neo
Alias: NM_027801.1;2610015P09Rik; 8430422M09Rik
Gene lenght: 2649bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ccdc191-EGFP-Neo plasmid Description

pCMV-Ccdc191-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ccdc191-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagGC CACCATGGAG CTGTCTCGGT GGAAAAGACT GAGCAGGAAA 661 CTGAACCTGA AAAGGGTGGA GAAGGCCTCT GAGTTCGCCA TCAGTAACAC CTGCTCTACA 721 AGGAATGCCG ACCTGCCCGG GAGGTTCTGG AGGCAGGCCG TGGACCTGGC CACCTGCGAG 781 GAGACCGAGT ATCATGACGA TGTGTATGAG GAAGCCCAGG AACTGGTGAA CGACTGGCTG 841 GACAACAAGC TGAAGCAGGA GCTGGCTAGC GACGGCGAGG GCGAGAGCAC AGTCCTGTCA 901 TCTCCTCCAG TGCCCGACGC TCACGACCAT CTGAAGTATG ACAAGTTTGA TGATCTGTGT 961 GGCTACCTGG AAGAAGAGGA GGAGTCTGCA ACAGTGCAGA AGTTCATCGA GCACCTGCTG 1021 CACAAGGAGG TGGTGGCCTC CGAGATGGTG GAGGAACTGG GAATGGAGGA GAATCAGGAC 1081 AAGAGGCAGC AGAAGGACCC TAGGCTGACA ATGGAGATGA GACACAAGCA GGTGAAGGAG 1141 AACAGGCTGC GCAGAGAGAA GCAGCTGGAG AGTCAGAGGA TGGCCGACGC ACTGAAGAAA 1201 GCAGCCTTCC TGGAGGCACA GAGCCTGGTG CAGGAGGAGA AACGGAGGAA GGCTCTGCTG 1261 GCCAAGAAGG AAAAGGAAGA CATTCAGCGC GAGATGGTGA AACTGAGAAG GGAAATCCTG 1321 GAGAGAAGGC ACACCGTGGA GGAGGCTTGG AAGACCGAGA AGAAGAGACA GGAGGAGATT 1381 AGCCAGAAGA ATTCTGAGGG CAAGATGATG GAAAGCACCC ACATCCACCT GGATGAGGAG 1441 AAGGTGGCTA AGGAGCGAAA ACGCAAGCTG CGGGAGACCC TGATCCAGAC ATTCAAGGAG 1501 AATCAGCAGT GTCAGAAGAG ATATTTCTCT GCCTGGCATA AGCTGATCCT GGATCACAGG 1561 ATCAAACTGG GTAAGGCTGG AACCCTGAGC GACTGGAAAc ttcaactgaa ggtcctgaga 1621 gcctggagag accatacacg atcccggaag ttggaacgag agaccaaagc cttggaaaat 1681 gaccttaggg aagaaaacag aaaacaacag ctggctactg agtttaaccg gaaacacgtt 1741 cttcgatact gctttggtga atggcagcgt tggcatggtt cagaagttat caagagggag 1801 ctggctctcg caaaggagca aactaggaag aaaatggatg agctgctgaa ggcagcctcc 1861 ctcgggaaac tcagttctaa ggggtcatca ggtatcggtc tcgttgctga ggcagtggct 1921 gtgatggacc cagcagtagg aagtggagag gtcactatcc tgtccccttt gtgggaaagg 1981 ccacctgtgg agagcagtgg ttgtacactc agccattccc cagaaagaac caccaagggt 2041 aattgtcagg gtcctctccc agatgtctct gggagcacac ctggcacgga gcagtgcaag 2101 gctgtagcag ctgagacctc tgagcagcct ggtagctgtg aacgacctgg gaccacaagg 2161 caggaggcag aagctgcttg tgtgaggcat ttccaccgta gccatgtgta ccagcaacag 2221 ctgattgaga agcaaaagaa gaaacttgag cagcagcaga aaaccattca gaagctaaag 2281 gaaaaccagc gcttggccga ggcccggtgg gcagctaagc gagccccggc agtcacagaa 2341 ggacagaact gccttcagtc agaccccaga ggagcaaggg acccgcaggg cacctgccag 2401 aggcttctga agtgtgaaga tagtagaact gattgcagaa aacccctttc tgcatccaga 2461 aggaccctga agcagccaac agcaccccat cccttactga aagccatgga agagagagca 2521 attcaacgag ctgaacgtag gcagatcttg gcagaaaaga agaaaaaaca agaagaagac 2581 aaattggctc agttaaaagc ccaagaggaa gaacgccaaa agaaggatgc agaagaaaag 2641 gaagcccagc tggagagaaa acgagaggaa aggaggctga agaagatgaa agaactagag 2701 aaacagaaga gaattaaaag aattcaagag ttggaagcag tagccaaagg tcattatgag 2761 agggtcttgc taaggaaaaa aggcctggag ccctggaaga gactgagaat gcaaagcaag 2821 cacaacattg aggtagcaga aaagtatcac tctttggctt tacaaagaaa atgcctgctc 2881 aactggttcc agtatagcca ggaaactctg gctatgaaga cagccaaggc tgatcaattt 2941 tactcccaac tgttgtatag gagaagcatc cggagctggc tccagtatgt cagtgatcta 3001 gagaaagaag tcaggagact ttgcacctac ttactgcaga agaagatttt caaggcctgg 3061 cttactactg tcagggagct gaagatggat tcgcaaagaa agcttaaggt tgccacagaa 3121 cactgtgaca ggaagattct catgctcacc ctggagatgt ggaaggcatt tgtaaaactt 3181 accaaagaag aaagagtgaa ggaagaacgc cgagaccacc tccgccggaa ggtctccgaa 3241 atccttccag acttccagat gctggtgcct ccgccgcggg cccgggatcc accggtcgcc 3301 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3361 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3421 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3481 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3541 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3601 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3661 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3721 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 3781 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 3841 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 3901 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 3961 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4021 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 4081 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 4141 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 4201 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 4261 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 4321 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 4381 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 4441 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 4501 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 4561 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 4621 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 4681 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 4861 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 4921 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 4981 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 5041 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 5101 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 5161 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 5221 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 5281 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 5341 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 5401 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 5461 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 5521 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 5581 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 5641 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 5701 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 5761 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 5821 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 5881 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 5941 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 6001 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 6061 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 6121 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 6181 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 6241 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 6301 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 6361 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 6421 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 6481 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 6541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 6601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 6661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 6721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 6781 gagcgcagat accaaatact gtTcttctag tgtagccgta gttaggccac cacttcaaga 6841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 6901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 6961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 7021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 7081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 7141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 7201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 7261 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 7321 cccctgattc tgtggataac cgtattaccg ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.