pLV3-CMV-SLC25A10-3×FLAG-CopGF

pLV3-CMV-SLC25A10-3×FLAG-CopGFP-Puro plasmid

PVT96815

pLV3-CMV-SLC25A10-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SLC25A10(human)-3×FLAG-CopGFP-Puro
Alias: NM_001270888.2;DIC; MTDPS19
Gene lenght: 888bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SLC25A10-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SLC25A10-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SLC25A10-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg cagccgaggc gcgcgtgtcg cgctggtact tcggggggct 2341 ggcctcctgc ggggccgcct gctgcacgca cccgctggac ctgctcaagg tgcatctgca 2401 gacgcagcag gaggtgaagc tgcgcatgac gggcatggcg ctgcgggtgg tgcgtaccga 2461 cggcatcctg gcactctaca gcggcctgag cgcctcgctg tgcagacaga tgacctactc 2521 cctgactcgg ttcgccatct acgagactgt gcgggaccgt gtggccaagg gcagccaggg 2581 gcctctcccc ttccacgaga aggtgttgct gggctccgtc agcggtttag ctggaggctt 2641 cgtggggacg cccgcagact tggtcaacgt caggatgcag aacgacgtga agctgcccca 2701 gggtcagcgg cgcaactacg cccatgcgct ggatggcctg taccgcgtag ctcgtgaaga 2761 gggtctcagg agactgttct cgggtgcaac catggcatcc agccgagggg ccttagtcac 2821 tgtgggccag ctgtcctgct acgaccaggc caagcagctg gtccttagca ccgggtacct 2881 ctctgacaac atcttcactc actttgtcgc cagctttatt gcagccgctg gtgacgagcc 2941 ccctcctcag ggtggatgtg ccacgttcct gtgccagccc ctggatgtgc tgaagactcg 3001 cctgatgaac tccaaggggg agtatcaggg cgttttccac tgcgccgtgg agacagcgaa 3061 gctcgggcct ctggcctttt acaagggcct cgtcccagct ggcatccgcc tcatccccca 3121 caccgtgctc acttttgtgt ttctggaaca gctacgcaaa aactttggca tcaaagtgcc 3181 atccggatcc GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA 3241 GGATGACGAT GACAAGTGAg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt 3301 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 3361 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 3421 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 3481 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 3541 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 3601 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 3661 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 3721 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 3781 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg 3841 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg 3901 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga 3961 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct 4021 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca 4081 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc 4141 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg 4201 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca 4261 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct 4321 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga 4381 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg 4441 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc 4501 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg 4561 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 4621 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 4681 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 4741 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 4801 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 4861 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 4921 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 4981 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5041 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5101 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 5161 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 5221 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 5281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 5341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 5401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 5461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 5521 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 5581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 5641 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 5701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 5761 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc 5821 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 5881 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 5941 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6001 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6061 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6121 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 6181 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 6241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 6301 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 6361 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 6421 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 6481 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 6541 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6601 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6721 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6781 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6901 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7321 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7681 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7741 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 7801 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 7861 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 7921 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 7981 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8041 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8101 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8161 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8221 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8281 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8341 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8401 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8461 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8521 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 8641 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 8701 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 8761 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 8821 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 8881 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 8941 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9001 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9061 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9121 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.