pLV2-CMV-BTRC-3×Myc-Puro plasm

pLV2-CMV-BTRC-3×Myc-Puro plasmid

PVT93412

pLV2-CMV-BTRC-3×Myc-Puro plasmid Information

Plasmid name: pLV2-CMV-BTRC(human)-3×Myc-Puro
Alias: NM_033637.4;BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;FBXW1; FBXW1A; FWD1
Gene lenght: 1815bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-BTRC-3×Myc-Puro plasmid Description

pLV2-CMV-BTRC-3×Myc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-BTRC-3×Myc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCATGGACCC GGCCGAGGCG GTGCTGCAAG AGAAGGCACT 2881 CAAGTTTATG TGCTCTATGC CCAGGTCTCT GTGGCTGGGC TGCTCCAGCC TGGCGGACAG 2941 CATGCCTTCG CTGCGATGCC TGTATAACCC AGGGACTGGC GCACTCACAG CTTTCCAGAA 3001 TTCCTCAGAG AGAGAAGACT GTAATAATGG CGAACCCCCT AGGAAGATAA TACCAGAGAA 3061 GAATTCACTT AGACAGACAT ACAACAGCTG TGCCAGACTC TGCTTAAACC AAGAAACAGT 3121 ATGTTTAGCA AGCACTGCTA TGAAGACTGA GAATTGTGTG GCCAAAACAA AACTTGCCAA 3181 TGGCACTTCC AGTATGATTG TGCCCAAGCA ACGGAAACTC TCAGCAAGCT ATGAAAAGGA 3241 AAAGGAACTG TGTGTCAAAT ACTTTGAGCA GTGGTCAGAG TCAGATCAAG TGGAATTTGT 3301 GGAACATCTT ATATCCCAAA TGTGTCATTA CCAACATGGG CACATAAACT CGTATCTTAA 3361 ACCTATGTTG CAGAGAGATT TCATAACTGC TCTGCCAGCT CGGGGATTGG ATCATATTGC 3421 TGAGAACATT CTGTCATACC TGGATGCCAA ATCACTATGT GCTGCTGAAC TTGTGTGCAA 3481 GGAATGGTAC CGAGTGACCT CTGATGGCAT GCTGTGGAAG AAGCTTATCG AGAGAATGGT 3541 CAGGACAGAT TCTCTGTGGA GAGGCCTGGC AGAACGAAGA GGATGGGGAC AGTATTTATT 3601 CAAAAACAAA CCTCCTGACG GGAATGCTCC TCCCAACTCT TTTTATAGAG CACTTTATCC 3661 TAAAATTATA CAAGACATTG AGACAATAGA ATCTAATTGG AGATGTGGAA GACATAGTTT 3721 ACAGAGAATT CACTGCCGAA GTGAAACAAG CAAAGGAGTT TACTGTTTAC AGTATGATGA 3781 TCAGAAAATA GTAAGCGGCC TTCGAGACAA CACAATCAAG ATCTGGGATA AAAACACATT 3841 GGAATGCAAG CGAATTCTCA CAGGCCATAC AGGTTCAGTC CTCTGTCTCC AGTATGATGA 3901 GAGAGTGATC ATAACAGGAT CATCGGATTC CACGGTCAGA GTGTGGGATG TAAATACAGG 3961 TGAAATGCTA AACACGTTGA TTCACCATTG TGAAGCAGTT CTGCACTTGC GTTTCAATAA 4021 TGGCATGATG GTGACCTGCT CCAAAGATCG TTCCATTGCT GTATGGGATA TGGCCTCCCC 4081 AACTGACATT ACCCTCCGGA GGGTGCTGGT CGGACACCGA GCTGCTGTCA ATGTTGTAGA 4141 CTTTGATGAC AAGTACATTG TTTCTGCATC TGGGGATAGA ACTATAAAGG TATGGAACAC 4201 AAGTACTTGT GAATTTGTAA GGACCTTAAA TGGACACAAA CGAGGCATTG CCTGTTTGCA 4261 GTACAGGGAC AGGCTGGTAG TGAGTGGCTC ATCTGACAAC ACTATCAGAT TATGGGACAT 4321 AGAATGTGGT GCATGTTTAC GAGTGTTAGA AGGCCATGAG GAATTGGTGC GTTGTATTCG 4381 ATTTGATAAC AAGAGGATAG TCAGTGGGGC CTATGATGGA AAAATTAAAG TGTGGGATCT 4441 TGTGGCTGCT TTGGACCCCC GTGCTCCTGC AGGGACACTC TGTCTACGGA CCCTTGTGGA 4501 GCATTCCGGA AGAGTTTTTC GACTACAGTT TGATGAATTC CAGATTGTCA GTAGTTCACA 4561 TGATGACACA ATCCTCATCT GGGACTTCCT AAATGATCCA GCTGCCCAAG CTGAACCCCC 4621 CCGTTCCCCT TCTCGAACAT ACACCTACAT CTCCAGAgga tccGAACAAA AACTAATATC 4681 AGAGGAAGAT TTGGAACAGA AACTAATCAG TGAAGAAGAT TTAGAGCAAA AGTTAATTTC 4741 CGAGGAGGAC TTATAGTGAt ctagataatt ctaccgggta ggggaggcgc ttttcccaag 4801 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct 4861 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg 4921 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct 4981 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga 5041 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg 5101 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg 5161 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct 5221 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg 5281 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5341 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5401 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5461 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5521 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5581 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5641 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5701 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5761 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5821 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5881 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag 5941 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 6001 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 6061 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 6121 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 6181 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 6241 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 6301 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 6361 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 6421 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct 6481 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca 6541 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag 6601 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag 6661 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac 6721 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct 6781 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc 6841 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc 6901 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag 6961 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac 7021 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg 7081 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct 7141 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7201 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7261 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7321 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 7381 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct 7441 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 7501 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7561 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 7621 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 7681 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7741 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7801 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 7861 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 7921 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 7981 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8041 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8101 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8161 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8221 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8281 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 8341 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8401 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8461 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8521 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8581 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8641 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8701 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8761 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 8821 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 8881 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 8941 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9001 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9061 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9121 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9181 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9241 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9301 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9361 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9421 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9481 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9601 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat 9661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 9721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg 9781 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc 9841 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 9901 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 9961 gttaaatatg tactacaaac ttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.