pLV3-CMV-KIT-3×FLAG-EF1a-CopGF

pLV3-CMV-KIT-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT96568

pLV3-CMV-KIT-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KIT(human)-3×FLAG-EF1a-CopGFP-Puro
Alias: BC071593.1;C-Kit
Gene lenght: 2928bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-KIT-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-KIT-3×FLAG-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KIT-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgagagg cgctcgcggc gcctgggatt ttctctgcgt 2341 tctgctccta ctgcttcgcg tccagacagg ctcttctcaa ccatctgtga gtccagggga 2401 accgtctcca ccatccatcc atccaggaaa atcagactta atagtccgcg tgggcgacga 2461 gattaggctg ttatgcactg atccgggctt tgtcaaatgg acttttgaga tcctggatga 2521 aacgaatgag aataagcaga atgaatggat cacggaaaag gcagaagcca ccaacaccgg 2581 caaatacacg tgcaccaaca aacacggctt aagcaattcc atttatgtgt ttgttagaga 2641 tcctgccaag cttttccttg ttgaccgctc cttgtatggg aaagaagaca acgacacgct 2701 ggtccgctgt cctctcacag acccagaagt gaccaattat tccctcaagg ggtgccaggg 2761 gaagcctctt cccaaggact tgaggtttat tcctgacccc aaggcgggca tcatgatcaa 2821 aagtgtgaaa cgcgcctacc atcggctctg tctgcattgt tctgtggacc aggagggcaa 2881 gtcagtgctg tcggaaaaat tcatcctgaa agtgaggcca gccttcaaag ctgtgcctgt 2941 tgtgtctgtg tccaaagcaa gctatcttct tagggaaggg gaagaattca cagtgacgtg 3001 cacaataaaa gatgtgtcta gttctgtgta ctcaacgtgg aaaagagaaa acagtcagac 3061 taaactacag gagaaatata atagctggca tcacggtgac ttcaattatg aacgtcaggc 3121 aacgttgact atcagttcag cgagagttaa tgattctgga gtgttcatgt gttatgccaa 3181 taatactttt ggatcagcaa atgtcacaac aaccttggaa gtagtagata aaggattcat 3241 taatatcttc cccatgataa acactacagt atttgtaaac gatggagaaa atgtagattt 3301 gattgttgaa tatgaagcat tccccaaacc tgaacaccag cagtggatct atatgaacag 3361 aaccttcact gataaatggg aagattatcc caagtctgag aatgaaagta atatcagata 3421 cgtaagtgaa cttcatctaa cgagattaaa aggcaccgaa ggaggcactt acacattcct 3481 agtgtccaat tctgacgtca atgctgccat agcatttaat gtttatgtga atacaaaacc 3541 agaaatcctg acttacgaca ggctcgtgaa tggcatgctc caatgtgtgg cagcaggatt 3601 cccagagccc acaatagatt ggtatttttg tccaggaact gagcagagat gctctgcttc 3661 tgtactgcca gtggatgtgc agacactaaa ctcatctggg ccaccgtttg gaaagctagt 3721 ggttcagagt tctatagatt ctagtgcatt caagcacaat ggcacggttg aatgtaaggc 3781 ttacaacgat gtgggcaaga cttctgccta ttttaacttt gcatttaaag gtaacaacaa 3841 agagcaaatc catccccaca ccctgttcac tcctttgctg attggtttcg taatcgtagc 3901 tggcatgatg tgcattattg tgatgattct gacctacaaa tatttacaga aacccatgta 3961 tgaagtacag tggaaggttg ttgaggagat aaatggaaac aattatgttt acatagaccc 4021 aacacaactt ccttatgatc acaaatggga gtttcccaga aacaggctga gttttgggaa 4081 aaccctgggt gctggagctt tcgggaaggt tgttgaggca actgcttatg gcttaattaa 4141 gtcagatgcg gccatgactg tcgctgtaaa gatgctcaag ccgagtgccc atttgacaga 4201 acgggaagcc ctcatgtctg aactcaaagt cctgagttac cttggtaatc acatgaatat 4261 tgtgaatcta cttggagcct gcaccattgg agggcccacc ctggtcatta cagaatattg 4321 ttgctatggt gatcttttga attttttgag aagaaaacgt gattcattta tttgttcaaa 4381 gcaggaagat catgcagaag ctgcacttta taagaatctt ctgcattcaa aggagtcttc 4441 ctgcagcgat agtactaatg agtacatgga catgaaacct ggagtttctt atgttgtccc 4501 aaccaaggcc gacaaaagga gatctgtgag aataggctca tacatagaaa gagatgtgac 4561 tcccgccatc atggaggatg acgagttggc catagactta gaagacttgc tgagcttttc 4621 ttaccaggtg gcaaagggca tggctttcct cgcctccaag aattgtattc acagagactt 4681 ggcagccaga aatatcctcc ttactcatgg tcggatcaca aagatttgtg attttggtct 4741 agccagagac atcaagaatg attctaatta tgtggttaaa ggaaacgctc gactacctgt 4801 gaagtggatg gcacatgaaa gcattttcaa ctgtgtatac acgtttgaaa gtgacgtctg 4861 gtcctatggg atttttcttt gggagctgtt ctctttagga agcagcccct atcctggaat 4921 gccggtcgat tctaagttct acaagatgat caaggaaggc ttccggatgc tcagccctga 4981 acacgcacct gctgaaatgt atgacataat gaagacttgc tgggatgcag atcccctaaa 5041 aagaccaaca ttcaagcaaa ttgttcagct aattgagaag cagatttcag agagcaccaa 5101 tcatatttac tccaacttag caaactgcag ccccaaccga cagaagcccg tggtagacca 5161 ttctgtgcgg atcaattctg tcggcagcac cgcttcctcc tcccagcctc tgcttgtgca 5221 cgacgatgtc GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA 5281 GGATGACGAT GACAAGTAGg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt 5341 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 5401 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 5461 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 5521 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc 5581 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc 5641 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca 5701 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct 5761 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc 5821 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg 5881 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg 5941 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga 6001 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct 6061 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca 6121 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc 6181 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg 6241 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca 6301 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct 6361 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga 6421 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg 6481 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc 6541 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg 6601 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg 6661 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6721 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6781 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6841 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6901 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6961 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 7021 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 7081 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 7141 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 7201 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 7261 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 7321 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 7381 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 7441 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 7501 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 7561 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 7621 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 7681 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 7741 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 7801 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc 7861 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 7921 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 7981 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 8041 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 8101 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 8161 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 8221 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 8281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 8341 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 8401 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 8461 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 8521 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 8581 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 8641 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 8701 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 9001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 9181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9361 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 9421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 9841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 10021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 10141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 10201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 10261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 10321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 10381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 10681 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 10741 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 10801 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 10861 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 10921 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 10981 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 11041 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 11101 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 11161 aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.