pLV3-CMV-BTRC-3×FLAG-mCherry-P

pLV3-CMV-BTRC-3×FLAG-mCherry-Puro plasmid

PVT96257

pLV3-CMV-BTRC-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-BTRC(human)-3×FLAG-mCherry-Puro
Alias: NM_033637.4;BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A;FBXW1; FBXW1A; FWD1
Gene lenght: 1815bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-BTRC-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-BTRC-3×FLAG-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-BTRC-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCATGGACCC GGCCGAGGCG GTGCTGCAAG AGAAGGCACT 2341 CAAGTTTATG TGCTCTATGC CCAGGTCTCT GTGGCTGGGC TGCTCCAGCC TGGCGGACAG 2401 CATGCCTTCG CTGCGATGCC TGTATAACCC AGGGACTGGC GCACTCACAG CTTTCCAGAA 2461 TTCCTCAGAG AGAGAAGACT GTAATAATGG CGAACCCCCT AGGAAGATAA TACCAGAGAA 2521 GAATTCACTT AGACAGACAT ACAACAGCTG TGCCAGACTC TGCTTAAACC AAGAAACAGT 2581 ATGTTTAGCA AGCACTGCTA TGAAGACTGA GAATTGTGTG GCCAAAACAA AACTTGCCAA 2641 TGGCACTTCC AGTATGATTG TGCCCAAGCA ACGGAAACTC TCAGCAAGCT ATGAAAAGGA 2701 AAAGGAACTG TGTGTCAAAT ACTTTGAGCA GTGGTCAGAG TCAGATCAAG TGGAATTTGT 2761 GGAACATCTT ATATCCCAAA TGTGTCATTA CCAACATGGG CACATAAACT CGTATCTTAA 2821 ACCTATGTTG CAGAGAGATT TCATAACTGC TCTGCCAGCT CGGGGATTGG ATCATATTGC 2881 TGAGAACATT CTGTCATACC TGGATGCCAA ATCACTATGT GCTGCTGAAC TTGTGTGCAA 2941 GGAATGGTAC CGAGTGACCT CTGATGGCAT GCTGTGGAAG AAGCTTATCG AGAGAATGGT 3001 CAGGACAGAT TCTCTGTGGA GAGGCCTGGC AGAACGAAGA GGATGGGGAC AGTATTTATT 3061 CAAAAACAAA CCTCCTGACG GGAATGCTCC TCCCAACTCT TTTTATAGAG CACTTTATCC 3121 TAAAATTATA CAAGACATTG AGACAATAGA ATCTAATTGG AGATGTGGAA GACATAGTTT 3181 ACAGAGAATT CACTGCCGAA GTGAAACAAG CAAAGGAGTT TACTGTTTAC AGTATGATGA 3241 TCAGAAAATA GTAAGCGGCC TTCGAGACAA CACAATCAAG ATCTGGGATA AAAACACATT 3301 GGAATGCAAG CGAATTCTCA CAGGCCATAC AGGTTCAGTC CTCTGTCTCC AGTATGATGA 3361 GAGAGTGATC ATAACAGGAT CATCGGATTC CACGGTCAGA GTGTGGGATG TAAATACAGG 3421 TGAAATGCTA AACACGTTGA TTCACCATTG TGAAGCAGTT CTGCACTTGC GTTTCAATAA 3481 TGGCATGATG GTGACCTGCT CCAAAGATCG TTCCATTGCT GTATGGGATA TGGCCTCCCC 3541 AACTGACATT ACCCTCCGGA GGGTGCTGGT CGGACACCGA GCTGCTGTCA ATGTTGTAGA 3601 CTTTGATGAC AAGTACATTG TTTCTGCATC TGGGGATAGA ACTATAAAGG TATGGAACAC 3661 AAGTACTTGT GAATTTGTAA GGACCTTAAA TGGACACAAA CGAGGCATTG CCTGTTTGCA 3721 GTACAGGGAC AGGCTGGTAG TGAGTGGCTC ATCTGACAAC ACTATCAGAT TATGGGACAT 3781 AGAATGTGGT GCATGTTTAC GAGTGTTAGA AGGCCATGAG GAATTGGTGC GTTGTATTCG 3841 ATTTGATAAC AAGAGGATAG TCAGTGGGGC CTATGATGGA AAAATTAAAG TGTGGGATCT 3901 TGTGGCTGCT TTGGACCCCC GTGCTCCTGC AGGGACACTC TGTCTACGGA CCCTTGTGGA 3961 GCATTCCGGA AGAGTTTTTC GACTACAGTT TGATGAATTC CAGATTGTCA GTAGTTCACA 4021 TGATGACACA ATCCTCATCT GGGACTTCCT AAATGATCCA GCTGCCCAAG CTGAACCCCC 4081 CCGTTCCCCT TCTCGAACAT ACACCTACAT CTCCAGAgga tccGACTACA AAGACCATGA 4141 CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG 4201 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc 4261 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt 4321 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc 4381 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac 4441 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 4501 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac 4561 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct 4621 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca 4681 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc 4741 gcctacgcta gacgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 4801 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 4861 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 4921 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 4981 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 5041 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 5101 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 5161 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 5221 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 5281 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 5341 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 5401 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 5461 gtacaaggga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa 5521 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 5581 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 5641 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 5701 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 5761 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 5821 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 5881 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 5941 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 6001 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 6061 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 6121 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 6181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 6241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 6301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 6361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 6421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 6481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 6541 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 6601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 6661 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct 6721 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 6781 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg 6841 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 6901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7021 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7081 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 7141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7201 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 7261 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 7321 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 7381 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 7441 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 7501 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 7561 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 7621 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7981 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8041 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8101 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8161 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8221 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 8281 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 8341 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 8401 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 8461 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8521 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 8581 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 8641 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 8701 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 8761 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8821 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 8881 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 8941 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9001 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9061 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9121 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9181 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9241 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9301 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9361 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9421 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9481 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9541 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 9601 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 9661 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 9721 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 9781 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 9841 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 9901 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 9961 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 10021 gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.