pLV3-CMV-Pdlim3-3×FLAG-CopGFP-

pLV3-CMV-Pdlim3-3×FLAG-CopGFP-Puro plasmid

PVT93960

pLV3-CMV-Pdlim3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pdlim3(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001374652.2;Actn2lp; ALP
Gene lenght: 1092bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pdlim3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pdlim3-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Pdlim3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc cccagaacgt agttctcccg 2341 ggtcctgcgc cctggggctt cagactctct gggggcatag acttcaatca gcctttggtc 2401 atcaccagga tcactccagg aagcaaagca gcagctgcca acctgtgtcc tggagatgtc 2461 atcctagcta ttgatggctt tggtacggag tctatgactc atgctgatgc acaggacagg 2521 attaaagcag cgtcctacca gttgtgtctc aagattgaca gggcagaaac tcgcttatgg 2581 tccccgcagg tgtctgaaga tggaaaagca caccccttca aaatcaactt agaagctgaa 2641 ccacaggatg tgaactactt tgaacacaag cacaatattc ggcccaaacc tttcataatt 2701 ccaggccgaa ccagtggctg cagcactcct tccggtgttg actgtggcag tggacgtagc 2761 accccttctt ccgtcagtac cgttagtaca atctgcccgg gtgacttgaa agttgctgct 2821 aagatggccc ctaacattcc tttggaaatg gaacttccgg gcgtgaagat cgtacacgct 2881 cagtttaaca cgcctatgca gttgtactca gatgacaata ttatggaaac acttcagggt 2941 caggttgcca cagctctagg ggagacatcc tccatgagtg aacccacagc ttcagtgccc 3001 cctcagtcgg acgtgtaccg catgctccat gacaaccggg atgacccagc cgctcctcgc 3061 cagtcgggct ccttcagggt gctgcaggat ctggtgaatg acgggcctga tgaccgtccg 3121 gctggaactc gaagcgtgag ggctccggtt acgaaagtcc acggtggggc tggcagtgcc 3181 cagaggatgc cgctctgtga caagtgtggg agtggtattg taggtgctgt cgtcaaggcc 3241 cgggataagt accggcaccc cgagtgcttt gtgtgtgccg actgtaacct caacctcaag 3301 cagaaaggct acttcttcgt ggagggggag ctgtattgtg agacgcacgc gagagcccgc 3361 acgaggcccc cagagggcta cgacaccgtc actctgtacc ccaaagctgg atccGACTAC 3421 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3481 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3541 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3601 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3661 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3721 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3781 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 3841 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 3901 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 3961 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4021 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4081 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4141 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4201 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4261 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 4321 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 4381 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 4441 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 4501 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 4561 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 4621 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 4681 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 4741 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 4801 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 4861 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 4921 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 4981 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5041 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5101 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5161 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5221 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 5281 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5341 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5401 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 5521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 5581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 5641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 5701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 5761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 5821 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 5881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 5941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 6001 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 6061 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 6121 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 6181 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6241 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6301 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6361 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6421 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 6481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 6541 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 6601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 6661 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6721 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 6781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 6841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 6901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 6961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 7141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 7201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 7261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 7321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 7381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 7441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 7501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 7561 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 7621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 7861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 7921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 7981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 8101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 8161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 8221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 8281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 8341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 8401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 8461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 8521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 8581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 8641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 8701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 8761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 8821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 8881 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 8941 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9001 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9061 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 9121 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 9181 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 9241 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 9301 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 9361 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.