pCMV-EGFP-EML2-Neo plasmid

pCMV-EGFP-EML2-Neo plasmid

PVT90562

pCMV-EGFP-EML2-Neo plasmid Information

Plasmid name: pCMV-EGFP-EML2(human)-Neo
Alias: NM_001193268.3;ELP70; EMAP-2; EMAP2
Gene lenght: 2553bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-EML2-Neo plasmid Description

pCMV-EGFP-EML2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-EML2-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT CATGCTGGAA CGAAGGGCGT TGCTATGGCA ACGGGAGGCA 1681 GGGCCTGGTT GGGGGGACCG GGCCCGGGCT GGGACCGGGG GGGCCGGCGG TGGCTGTGGT 1741 GGGGCGATGG CGGAGCGCGG CCCGGCCTTC TGCGGCCTGT ACGACACGTC CTCGCTGCTG 1801 CGATACTGCA ACGATGACAA TTTGTCGGGC ACGAGCGGTA TGGAAGTGGA CGACCGCGTG 1861 TCGGCGCTGG AGCAGCGGCT GCAGTTACAG GAAGACGAGC TGGCGGTCCT AAAGGCGGCG 1921 CTGGCGGATG CTCTGCGTCG CCTGCGGGCA TGCGAAGAAC AGGGAGCGGC GCTACGCGCG 1981 CGGGGCACCC CCAAGGGCCG GGCGCCTCCG CGCTTAGGCA CCACTGCCTC GGTGTGTCAG 2041 CTCTTGAAAG GCCTTCCCAC CAGGACGCCC CTTAATGGCT CGGGACCCCC GCGGCGCGTG 2101 GGTGGCTATG CCACGTCCCC ATCCTCTCCC AAGAAGGAGG CGACCTCCGG GCGCAGCAGT 2161 GTCCGCCGCT ACTTGTCACC AGAGCGCCTC GCCTCGGTGC GCCGTGAGGA CCCCCGCAGC 2221 CGGACCACAT CCTCCAGCAG CAACTGTAGC GCCAAAAAGG AAGGCAAAAC CAAAGAAGTT 2281 ATCTTCAGTG TGGAGGATGG CTCCGTGAAA ATGTTCCTGA GGGGCCGCCC TGTGCCCATG 2341 ATGATCCCAG ACGAGCTGGC ACCCACCTAC AGCCTGGACA CACGCTCGGA GCTGCCTTCT 2401 TGCCGGCTCA AGCTGGAGTG GGTCTATGGC TACCGTGGCC GAGACTGCCG GGCCAACCTT 2461 TATTTGCTGC CCACCGGGGA GATAGTGTAC TTTGTGGCCT CCGTAGCCGT GCTATACAGC 2521 GTGGAGGAGC AGAGGCAGCG ACACTACCTG GGACACAACG ATGACATCAA ATGCTTGGCC 2581 ATCCACCCAG ATATGGTCAC CATCGCCACG GGACAGGTGG CGGGAACCAC TAAGGAAGGG 2641 AAGCCGCTGC CGCCCCACGT GCGCATCTGG GACTCAGTTT CCCTCTCCAC CTTACACGTG 2701 CTGGGCTTGG GGGTGTTTGA CAGAGCCGTG TGCTGTGTGG GCTTCTCCAA ATCTAATGGA 2761 GGCAACCTGC TGTGTGCAGT GGATGAATCC AATGATCACA TGCTCTCGGT GTGGGACTGG 2821 GCCAAGGAGA CCAAGGTGGT GGATGTCAAG TGCTCCAATG AGGCTGTATT GGTGGCCACC 2881 TTCCACCCCA CGGACCCCAC TGTGCTTATC ACCTGCGGGA AATCTCACAT CTACTTCTGG 2941 ACCTTGGAGG GGGGCAGCTT GAGCAAGCGG CAAGGCCTCT TTGAGAAACA TGAGAAACCG 3001 AAGTATGTGC TGTGTGTGAC CTTTTTGGAA GGTGGCGACG TGGTCACGGG GGACTCTGGG 3061 GGGAACCTCT ATGTTTGGGG CAAAGGTGGG AACCGTATCA CACAGGCGGT GCTGGGCGCC 3121 CACGACGGCG GCGTGTTTGG GCTCTGCGCC CTGCGGGACG GGACGCTGGT GTCTGGAGGG 3181 GGCCGTGATC GGCGGGTGGT CCTCTGGGGT TCTGACTACA GCAAGCTGCA GGAAGTGGAG 3241 GTCCCTGAGG ACTTTGGCCC TGTGCGCACC GTGGCAGAGG GCCACGGAGA CACACTGTAC 3301 GTGGGGACCA CCCGCAATTC CATCCTGCAG GGCTCCGTGC ACACAGGCTT CTCACTGCTG 3361 GTCCAGGGCC ATGTGGAAGA GCTGTGGGGC CTGGCCACAC ACCCCAGTCG GGCCCAGTTT 3421 GTGACCTGCG GGCAGGATAA GCTGGTGCAT CTATGGAGCT CAGATTCCCA CCAGCCCCTG 3481 TGGAGCAGGA TCATCGAGGA CCCTGCCCGC TCAGCCGGCT TCCACCCCAG TGGCTCTGTC 3541 CTGGCTGTGG GTACAGTGAC TGGCAGATGG CTGCTGCTGG ACACGGAGAC CCATGACCTG 3601 GTGGCTATCC ACACAGACGG CAATGAACAG ATCTCAGTGG TCAGCTTCTC CCCAGACGGG 3661 GCGTACCTGG CCGTGGGCTC CCACGACAAC TTGGTGTACG TGTACACGGT GGACCAGGGC 3721 GGCCGCAAGG TCAGCCGCCT GGGCAAGTGC TCGGGCCATT CCAGTTTTAT CACCCACCTG 3781 GATTGGGCCC AGGACAGCAG CTGCTTTGTC ACCAACTCCG GGGACTATGA GATTCTGTAC 3841 TGGGACCCGG CTACCTGTAA GCAGATCACC AGTGCGGATG CTGTGAGGAA CATGGAATGG 3901 GCCACAGCTA CTTGTGTCCT AGGGTTTGGG GTGTTTGGGA TCTGGTCTGA GGGGGCGGAC 3961 GGCACTGATA TCAACGCTGT GGCCCGCTCT CATGATGGGA AGTTGCTGGC TTCAGCTGAT 4021 GACTTTGGCA AAGTTCACCT GTTTAGCTAC CCCTGCTGTC AGCCTCGAGC CCTCAGCCAC 4081 AAGTACGGTG GACACAGCAG CCATGTGACA AATGTGGCCT TCTTGTGGGA TGACAGCATG 4141 GCCCTGACCA CAGGGGGCAA GGACACCAGT GTGCTACAGT GGCGGGTGGT CTGAGGATCC 4201 tGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA 4261 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 4321 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 4381 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 4441 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG 4501 GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA 4561 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 4621 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 4681 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 4741 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 4801 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 4861 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 4921 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG 4981 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 5041 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 5101 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 5161 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 5221 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 5281 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 5341 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 5401 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 5461 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 5521 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 5581 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 5641 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 5701 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 5761 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 5821 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 5881 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 5941 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 6001 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 6061 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 6121 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 6181 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 6241 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 6301 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 6361 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 6421 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 6481 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 6541 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 6601 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 6661 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6721 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6781 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6841 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 6901 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 6961 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 7021 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 7081 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 7141 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 7201 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 7261 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 7321 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 7381 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 7441 CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 7501 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 7561 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 7621 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 7681 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 7741 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 7801 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 7861 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 7921 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 7981 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 8041 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 8101 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 8161 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 8221 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 8281 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 8341 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 8401 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 8461 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 8521 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 8581 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 8641 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 8701 T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.