pCMV-SRSF9-EGFP-Neo plasmid

pCMV-SRSF9-EGFP-Neo plasmid

PVT95797

pCMV-SRSF9-EGFP-Neo plasmid Information

Plasmid name: pCMV-SRSF9(human)-EGFP-Neo
Alias: NM_003769.3;SFRS9; SRp30c
Gene lenght: 666bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SRSF9-EGFP-Neo plasmid Description

pCMV-SRSF9-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SRSF9-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgtcgggct gggcggacga 661 gcgcggcggc gagggcgacg ggcgcatcta cgtggggaac cttccgaccg acgtgcgcga 721 gaaggacttg gaggacctgt tctacaagta cggccgcatc cgcgagatcg agctcaagaa 781 ccggcacggc ctcgtgccct tcgccttcgt gcgcttcgag gacccccgag atgcagagga 841 tgctatttat ggaagaaatg gttatgatta tggccagtgt cggcttcgtg tggagttccc 901 caggacttat ggaggtcggg gtgggtggcc ccgtggtggg aggaatgggc ctcctacaag 961 aagatctgat ttccgagttc ttgtttcagg acttcctccg tcaggcagct ggcaggacct 1021 gaaggatcac atgcgagaag ctggggatgt ctgttatgct gatgtgcaga aggatggagt 1081 ggggatggtc gagtatctca gaaaagaaga catggaatat gccctgcgta aactggatga 1141 caccaaattc cgctctcatg agggtgaaac ttcctacatc cgagtttatc ctgagagaag 1201 caccagctat ggctactcac ggtctcggtc tgggtcaagg ggccgtgact ctccatacca 1261 aagcaggggt tccccacact acttctctcc tttcaggccc tacggtggag gcggatcagg 1321 aggcggtgga tctatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 1441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 1501 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 1621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 1741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 1801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 1861 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 1921 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 1981 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 2041 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag 2101 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 2161 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 2221 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 2281 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 2341 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 2401 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 2461 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 2521 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 2581 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 2641 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 2701 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 2761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 2821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 2881 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 2941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3001 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3061 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 3121 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 3181 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 3241 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3301 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3361 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3421 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 3481 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3541 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3601 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3661 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 3721 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 3781 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 3841 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 3901 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 3961 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4021 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4081 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4141 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4201 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 4261 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 4321 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 4381 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 4441 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 4501 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4801 ggcttcagca gagcgcagat accaaatact gtTcttctag tgtagccgta gttaggccac 4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 5341 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.