pCMV-Abhd5-FKBP1A-mCherry-Neo

pCMV-Abhd5-FKBP1A-mCherry-Neo plasmid

PVT94193

pCMV-Abhd5-FKBP1A-mCherry-Neo plasmid Information

Plasmid name: pCMV-Abhd5(mouse)(19-35aa)-FKBP1A(human)-mCherry-Neo
Alias: NM_000801.5;FKBP-12; FKBP-1A; FKBP1; FKBP12; PKC12;PKCI2; PPIASE
Gene lenght: 324bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human,Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Abhd5-FKBP1A-mCherry-Neo plasmid Description

pCMV-Abhd5-FKBP1A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Abhd5-FKBP1A-mCherry-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC ATGtcaggat ggctgacagg 661 gtggcttcct acctggtgtc ccacatctac atcaGACCCG ACGCGCTCCG CCAACTCTGG 721 CGCCGGGGCT GGTGCCGGCG CAATCCTTTC CCGAatggga gtgcaggtgg aaaccatctc 781 cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg 841 gatgcttgaa gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt 901 tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt 961 gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc 1021 aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaagg 1081 tggaggcgga tcaggaggcg gtggatctgc ggatccaccg gtcgccacca tggtgagcaa 1141 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 1201 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 1261 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 1321 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 1381 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 1441 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 1501 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 1561 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 1621 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 1681 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 1741 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 1801 ccgccactcc accggcggca tggacgagct gtacaagtag cggccgcgac tctagatcat 1861 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 1921 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg 2101 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 2161 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 2221 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 2281 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 2341 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 2401 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 2461 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 2521 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 2581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 2641 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 2701 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 2761 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 2821 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2881 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2941 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 3001 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 3061 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 3121 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 3181 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 3241 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 3301 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 3361 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 3421 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 3481 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 3541 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 3601 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 3661 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 3721 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 3781 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 3841 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 3901 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3961 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 4021 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 4081 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 4141 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 4201 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 4261 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 4321 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 4381 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 4441 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 4501 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 4561 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 4621 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 4681 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 4741 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 4801 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4861 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4921 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 4981 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 5041 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 5101 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 5161 taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.