pLV3-CMV-YAP1-3×Myc-Hygro plas

pLV3-CMV-YAP1-3×Myc-Hygro plasmid

PVT95075

pLV3-CMV-YAP1-3×Myc-Hygro plasmid Information

Plasmid name: pLV3-CMV-YAP1(human)-3×Myc-Hygro
Alias: NM_001130145.3;COB1; YAP; YAP-1; YAP2; YAP65; YKI
Gene lenght: 1512bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-YAP1-3×Myc-Hygro plasmid Description

pLV3-CMV-YAP1-3×Myc-Hygro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-YAP1-3×Myc-Hygro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCa tggatcccgg gcagcagccg ccgcctcaac cggcccccca gggccaaggg 2341 cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg gcaaccggca 2401 cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat cgtgcacgtc 2461 cgcggggact cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa ccccaagacg 2521 gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc cttcttcaag 2581 ccgccggagc ccaaatccca ctcccgacag gccagtactg atgcaggcac tgcaggagcc 2641 ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt gggagctgtt 2701 tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac acccacagct 2761 cagcatcttc gacagtcttc ttttgagata cctgatgatg tacctctgcc agcaggttgg 2821 gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga tcagacaaca 2881 acatggcagg accccaggaa ggccatgctg tcccagatga acgtcacagc ccccaccagt 2941 ccaccagtgc agcagaatat gatgaactcg gcttcaggtc ctcttcctga tggatgggaa 3001 caagccatga ctcaggatgg agaaatttac tatataaacc ataagaacaa gaccacctct 3061 tggctagacc caaggcttga ccctcgtttt gccatgaacc agagaatcag tcagagtgct 3121 ccagtgaaac agccaccacc cctggctccc cagagcccac agggaggcgt catgggtggc 3181 agcaactcca accagcagca acagatgcga ctgcagcaac tgcagatgga gaaggagagg 3241 ctgcggctga aacagcaaga actgcttcgg caggcaatgc ggaatatcaa tcccagcaca 3301 gcaaattctc caaaatgtca ggagttagcc ctgcgtagcc agttaccaac actggagcag 3361 gatggtggga ctcaaaatcc agtgtcttct cccgggatgt ctcaggaatt gagaacaatg 3421 acgaccaata gctcagatcc tttccttaac agtggcacct atcactctcg agatgagagt 3481 acagacagtg gactaagcat gagcagctac agtgtccctc gaaccccaga tgacttcctg 3541 aacagtgtgg atgagatgga tacaggtgat actatcaacc aaagcaccct gccctcacag 3601 cagaaccgtt tcccagacta ccttgaagcc attcctggga caaatgtgga ccttggaaca 3661 ctggaaggag atggaatgaa catagaagga gaggagctga tgccaagtct gcaggaagct 3721 ttgagttctg acatccttaa tgacatggag tctgttttgg ctgccaccaa gctagataaa 3781 gaaagctttc ttacatggtt aCTCGAGGCC ACCatgGAAC AAAAACTAAT ATCAGAGGAA 3841 GATTTGGAAC AGAAACTAAT CAGTGAAGAA GATTTAGAGC AAAAGTTAAT TTCCGAGGAG 3901 GACTTAtagg atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag 3961 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg 4021 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 4081 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 4141 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac 4201 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc 4261 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga 4321 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc 4381 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt 4441 acagatccaa gctgtgaccg gcgcctacgt cgacatgaaa aagcctgaac tcaccgcgac 4501 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 4561 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 4621 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 4681 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 4741 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 4801 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 4861 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 4921 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 4981 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 5041 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 5101 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 5161 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 5221 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 5281 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 5341 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 5401 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 5461 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gtcgacaatc aacctctgga 5521 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5581 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5641 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5701 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5761 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5821 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5881 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 5941 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6001 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 6061 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt 6121 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 6181 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 6241 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 6301 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 6361 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 6421 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 6481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 6541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6601 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 6661 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 6721 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 6781 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6841 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6901 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6961 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7621 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7741 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7861 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7921 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7981 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8041 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8101 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8161 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8221 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8281 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8341 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8401 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8461 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8521 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8581 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8641 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8701 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8761 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8821 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8881 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8941 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9001 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9061 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9121 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9181 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9241 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9301 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9361 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.