pCMV-EGFP-Prkcg-3×HA-Neo plasm

pCMV-EGFP-Prkcg-3×HA-Neo plasmid

PVT86719

pCMV-EGFP-Prkcg-3×HA-Neo plasmid Information

Plasmid name: pCMV-EGFP-Prkcg(mouse)-3×HA-Neo
Alias: NM_011102.4;Pkcc; PKCgamma; Prkcc;
Gene lenght: 2091bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-EGFP-MCS-3×HA-Neo
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Prkcg-3×HA-Neo plasmid Description

pCMV-EGFP-Prkcg-3×HA-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of the target gene with a 3×HA tag, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation. The plasmid carries an EGFP green fluorescent protein at the N-terminus, allowing for visual observation of the location and level of target gene expression. The plasmid also carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-EGFP-Prkcg-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC 901 ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC 961 GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC 1021 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG 1081 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG 1141 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC 1201 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC 1261 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC 1321 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC 1381 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC 1441 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC 1501 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 1561 ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCTCGAGG CGGCGGAGGG 1621 AGTGGAGGAG GGGGAAGCGG AGGCGGAGGG AGTAAGCTTA TGGCGGGTCT GGGCCCTGGC 1681 GGAGGCGACT CAGAGGGGGG ACCCCGACCC CTGTTTTGCA GAAAGGGGGC GCTGAGGCAG 1741 AAGGTGGTCC ACGAGGTGAA GAGCCACAAG TTCACCGCTC GTTTCTTCAA GCAGCCAACC 1801 TTCTGCAGTC ACTGTACCGA CTTCATCTGG GGCATTGGAA AGCAGGGCCT GCAATGTCAA 1861 GTCTGTAGCT TTGTGGTTCA CCGCCGATGC CACGAATTTG TGACCTTCGA GTGTCCAGGC 1921 GCTGGAAAGG GCCCCCAGAC GGACGACCCT CGCAACAAGC ACAAGTTCCG TCTGCACAGC 1981 TACAGCAGTC CCACCTTCTG CGACCACTGT GGCTCCCTCC TTTACGGGCT GGTGCACCAG 2041 GGCATGAAAT GTTCCTGTTG CGAGATGAAT GTGCACCGGC GCTGTGTGCG CAGCGTGCCC 2101 TCCCTTTGCG GTGTGGACCA CACAGAGCGC CGTGGACGTC TGCAACTGGA AATCCGGGCT 2161 CCTACGTCGG ATGAGATCCA TATTACTGTT GGCGAGGCCC GGAACCTCAT TCCTATGGAC 2221 CCCAATGGCC TGTCTGATCC CTACGTGAAA CTGAAGCTGA TCCCGGACCC TCGGAACCTG 2281 ACAAAACAGA AGACAAAGAC CGTGAAAGCC ACACTGAATC CCGTGTGGAA CGAGACCTTC 2341 GTGTTCAACC TGAAGCCAGG GGATGTAGAG CGCCGGCTCA GTGTGGAGGT GTGGGATTGG 2401 GATAGGACAT CCCGAAATGA CTTCATGGGT GCCATGTCCT TTGGTGTCTC AGAGCTACTC 2461 AAGGCCCCTG TGGATGGATG GTACAAGTTA CTGAACCAGG AGGAGGGCGA ATATTACAAT 2521 GTACCGGTGG CCGATGCTGA CAACTGCAGC CTCCTCCAGA AGTTTGAGGC CTGCAATTAC 2581 CCCTTGGAAT TGTATGAGAG AGTGCGGATG GGCCCCTCTT CCTCCCCCAT CCCTTCTCCA 2641 TCCCCCAGTC CTACGGACTC CAAGCGATGC TTCTTTGGTG CCAGCCCAGG ACGCCTGCAT 2701 ATCTCTGACT TCAGCTTCCT CATGGTTCTA GGGAAAGGCA GTTTTGGGAA GGTAATGCTG 2761 GCAGAGCGCA GAGGCTCCGA CGAACTCTAT GCCATCAAGA TACTGAAGAA AGACGTCATC 2821 GTCCAGGATG ACGATGTAGA CTGCACCCTC GTAGAGAAGC GTGTCCTGGC ATTGGGAGGC 2881 CGAGGTCCTG GAGGCCGGCC ACACTTTCTC ACGCAGCTTC ACTCCACCTT TCAGACTCCG 2941 GACCGCCTGT ATTTTGTGAT GGAGTATGTC ACTGGGGGCG ATTTAATGTA CCACATCCAG 3001 CAACTGGGCA AGTTTAAGGA GCCTCATGCA GCATTCTACG CTGCGGAAAT CGCCATAGGC 3061 CTCTTCTTCC TTCACAACCA GGGCATCATC TACAGGGACC TCAAGTTGGA TAATGTGATG 3121 CTGGATGCTG AAGGACACAT CAAGATCACA GACTTTGGCA TGTGTAAAGA GAATGTCTTC 3181 CCTGGGTCCA CAACCCGCAC CTTCTGTGGC ACCCCAGACT ACATAGCACC TGAGATCATT 3241 GCCTATCAGC CCTACGGGAA GTCTGTCGAC TGGTGGTCTT TTGGGGTCCT GCTGTATGAG 3301 ATGTTGGCAG GACAGCCACC CTTTGATGGG GAAGATGAGG AAGAGTTGTT TCAAGCCATC 3361 ATGGAACAAA CTGTCACCTA TCCCAAGTCA CTTTCCCGGG AAGCTGTGGC CATCTGCAAA 3421 GGGTTCCTGA CCAAGCACCC AGGAAAACGC CTGGGCTCAG GGCCAGATGG GGAACCCACC 3481 ATCCGGGCTC ATGGCTTTTT CCGTTGGATC GATTGGGAGA GGTTGGAGAG ACTGGAAATT 3541 GCACCTCCTT TCAGACCACG TCCGTGTGGC CGCAGTGGCG AAAACTTTGA CAAGTTCTTC 3601 ACGCGGGCAG CGCCAGCACT GACCCCGCCA GACCGCTTGG TTCTAGCCAG CATCGACCAA 3661 GCTGATTTCC AGGGCTTTAC TTATGTGAAC CCGGACTTCG TGCACCCAGA TGCCCGCAGC 3721 CCCACAAGCC CTGTGCCCGT GCCTGTCATG GGTACCTACC CATACGACGT ACCTGATTAT 3781 GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT CCCAGACTAC 3841 GCATAGGATC CACTAGTCCA GTGTGGTGGA ATTCTGCAGA TATCCAGCAC AGTGGCGGCC 3901 GCTCGAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG 3961 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 4021 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 4081 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 4141 GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC 4201 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 4261 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 4321 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 4381 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 4441 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 4501 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 4561 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 4621 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 4681 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 4741 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 4801 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 4861 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 4921 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 4981 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 5041 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 5101 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 5161 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 5221 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 5281 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 5341 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 5401 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 5461 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 5521 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 5581 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 5641 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 5701 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 5761 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 5821 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 5881 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 5941 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 6001 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 6061 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 6121 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 6181 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 6241 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 6301 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 6361 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 6421 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 6481 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 6541 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 6601 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 6661 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 6721 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 6781 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 6841 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 6901 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 6961 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 7021 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 7081 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 7141 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 7201 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 7261 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 7321 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 7381 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 7441 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 7501 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 7561 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 7621 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 7681 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 7741 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 7801 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 7861 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 7921 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 7981 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 8041 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 8101 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 8161 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 8221 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 8281 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 8341 ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.