pSV40-hRluc-Aldh2-3UTR-2-HSVTK

pSV40-hRluc-Aldh2-3UTR-2-HSVTK-Fluc plasmid

PVT86420

pSV40-hRluc-Aldh2-3UTR-2-HSVTK-Fluc plasmid Information

Plasmid name: pSV40-hRluc-Aldh2(mouse)-3UTR-2-HSVTK-Fluc
Alias: NM_009656.4;Ahd-5; AHD-M1; Ahd5; ALDH-E2; ALDHI;
Gene lenght: 1964bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: UTR
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc,hRluc
Promoter: pSV40
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: psiCHECK-2
Forward primer: hRluc-F1
Reverse primer:
Copy number: High
Induction:

pSV40-hRluc-Aldh2-3UTR-2-HSVTK-Fluc plasmid Description

pSV40-hRluc-Aldh2-3UTR-2-HSVTK-Fluc This plasmid is suitable for dual-luciferase detection in mammalian cells. The pSV40 promoter carried by the plasmid can initiate the transcription of the Fluc fluorescent marker and the 3' UTR region of the Aldh2 gene. The plasmid has two fluorescent reporter genes, firefly luciferase (Fluc) and Renilla luciferase (Rluc). The expression of Fluc is influenced by the inserted miRNA target sequence, and the predicted miRNA target sequence is often inserted into the 3' UTR region of the Fluc gene to verify the activity of the miRNA target gene, while Rluc serves as a reference signal to correct experimental errors.

pSV40-hRluc-Aldh2-3UTR-2-HSVTK-Fluc plasmid Sequence

1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT 61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA 481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT 541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC 601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA 661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG 721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG 781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT 841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC 901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT 961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC 1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC 1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG 1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA 1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG 1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG 1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC 1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT 1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA 1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG 1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC 1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGAG CGGCATGCCT GCTTCCTCAG CCCGCACCCG 1681 AAAACCCAAC AAGATATACT GAGAAAAACC GCCACACACA CTGCGCCTCC AAAGAGAAAC 1741 CCCTTCACCA AAGTGTCTTG GGTCAAGAAA GAATTTTATA AACAGGGCGG GGCTGGTGGG 1801 GGGGAAAGCT CCTGATAAAC TGGGTAGGGG ATGAAGCTCA ATGCAGACCG ATCACGCGTC 1861 CAGATGTGCA GGATGCTGCC TTCAACCTGC AGTCCCTAAG CAGCAAATGA GCAATAAAAA 1921 TCAGCAGATC AAAGCCACGG GGTCAGTTCT CTAAGACGTA AATTCTGAGT CTTATCTCTG 1981 TTGCATTCCG TAACTCTCTG CTTTGGGAGG AGACAAGGCC GTCCTTAGAA TTGAATTAGC 2041 TCTGTGGAAC ACTAGCGCCT TGGTGTTTAC TGGTCAGAAG TCATGAAAGG CAGGACCCCT 2101 CTCTATTCCT GGATACACGG GACGCCAGGA TGTCCCCACT ATTTGGTGAT ATCATGTATA 2161 TCTCATTACC CTCATCCCCA TCTTGGTACC TGGGATCTTG GTTCTCACAA GTAATTCTAG 2221 GCTGACGAGG AGGGATGTAA GCTAAAGGGA GGGGAGTCAC TATTCTTGGC TGCAGCTACA 2281 TTTTGGAATT TCACACTGGC CTATCTCACA GGCCAGAGGA GGTAGTGACA CCCGTTTGAT 2341 CCTTTCCAAG GGTGAGCCAG GTTAGGTTTC AACCAAGGGA CCTCACGGCT CGGCATCAGT 2401 TGCATGCTGC TAACTAACCT GGAGTCGATT CAGCCAAAAG ACAGTTTCCA GAAGTGGCTC 2461 TGTTCTCCCA ATCTGTTCTG CGGCCTCTTT GAGGCACAGA GCAGAGCAGA GCCATGTCAT 2521 GTGCACAGTG CAAGGTCTGC CTCTCGAACT ACCAGAACCA AGAGAGATTG GAGGTATTAG 2581 TGACAGTGGC CTGATGGAGG TGGCAGGTGA TGGCCCGTGA AGGTAGATTT TCAGGGAATA 2641 AGAGAATGTG GCAGTGACTG GCAGCTGCAA TGCAGACTCT GGTTCAGGCT ATGGTCACTG 2701 CAGATGCCTG GATGTGTGAC AGATGTGATG ACATTGCTGA AAGACAGAGG GACCCACTCC 2761 CAGTGGAAGG CCAAGAGAGC TCTTGAGCAG GGCTCCAGTT ACGTGAGCTT TCTCCCTCTT 2821 GTACCAGAAG GTTCCATTCC TAGAGGTTTT AGCTACCTGT GACCTCTGCC CTGTGTATAA 2881 ATGGGGTTAC TGTGACTATC CCAACCCTGC CCTGTCCTAA AGTACCTTCA AGGGCCACCT 2941 TGTGCCCAAC CTGTAGATTA TTCCCTATAC AGAAATGCTC ATGATGTTTG GCATAAAAAA 3001 AAAATTAAGC CAGCAATTAG CAATAGTCAT TGTGAGAAGT TACACAACTC TTGGTGCCGC 3061 TACGCTTGTG TTTTGGGGTG TTGAGTTAGC TCTGCATGGG TGCTGCAATA CTCAATCCTG 3121 GAAGCCCAGG AGCCAAGATG GTTGCTCGTA GCTGATAGGT GGGCAAATAT GCCACGTGGG 3181 TACAACGGCT GCAGGCATGA TGCTCACATC GGGCACAGGG CCGCACCGAT GCATGCAGCC 3241 TGGACCAGTG TGTGCTTATT TTCAGAATTT CCGTTTATAC TCTTATGCTA TGCATGACGA 3301 TTAGCTGCCT GGTACCTGCC CTGAGCAGGG ATAAGCAGGT CAAGTCCAAG GTCCACAGGA 3361 GCTGGGAAGC TTAGGGGCGA AGGTCACAGA TCTTACTCAC CTAGTGAGTG AACAAGGCGT 3421 GGAGAGCAAG CTGCCATCAC AGGCACAAGA AACGGACGGT GAGCTTAGCT TTAGAACTAG 3481 CCAGTCAGAG GCAGAGCTGA GGGTAGAAGG CTGATGAAGC CCTGAAGTTG TCCTTCGACC 3541 TCCATATACA CATCCCTGTA TGTGCATGCG CACTCAATGA AATAAATAAG TAAATACAAT 3601 TTTTAAAGAT CAGCGGCCGC TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 3661 GTTGGTTTTT TGTGTGAGGA TCTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG 3721 GTGGTTAGGG TTTGTCTGAC GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG 3781 GCGGCTCGGG GTTTGGTCTT GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA 3841 AGCACCCCCC CGCCCTCCGT GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC 3901 GCTGACTCGG GCGGGTCGCG CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC 3961 CGGGCGGCGC CGCCGCGGCG GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA 4021 TACGTGGACA GGCTCTGGAG CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC 4081 GGGACGAGCC GGGTCACGCG GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG 4141 GGGCACAGGT ACACTATCTT GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG 4201 TGGCGCAGCT GCTTCATCCC CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA 4261 GAAATATATT TGCATGTCTT TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA 4321 TTGGCGAATT CGAACACGCA GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT 4381 ATTAAGGTGA CGCGTGTGGC CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC 4441 TTGGCATTCC GGTACTGTTG GTAAAGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG 4501 CCCTGCTCCC TTCTACCCTC TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT 4561 GAAGAGGTAT GCCCTGGTGC CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA 4621 CATCACCTAT GCCGAGTACT TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA 4681 CGGCCTGAAC ACCAACCACC GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT 4741 GCCAGTGCTG GGCGCCCTGT TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA 4801 CGAGCGCGAG CTGCTGAACA GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA 4861 GAAGGGCCTG CAGAAGATCC TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT 4921 CATCATGGAC TCTAAGACCG ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC 4981 TCATCTGCCT CCTGGCTTCA ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA 5041 AACCATTGCC CTGATCATGA ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT 5101 GCCTCATCGC ACCGCCTGTG TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA 5161 GATCATCCCC GACACCGCTA TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT 5221 CACCACCCTG GGCTACCTGA TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA 5281 GGAGCTGTTC CTGCGCAGCC TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC 5341 CCTGTTCAGC TTCTTCGCTA AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA 5401 CGAGATTGCC TCTGGCGGCG CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG 5461 CTTTCATCTG CCAGGCATCC GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT 5521 GATTACCCCA GAGGGCGACG ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA 5581 GGCCAAGGTG GTGGACCTGG ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT 5641 GTGTGTGCGC GGCCCTATGA TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC 5701 CCTGATCGAC AAGGACGGCT GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA 5761 GCACTTCTTC ATCGTGGACC GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC 5821 CCCAGCCGAG CTGGAGTCTA TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC 5881 CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG 5941 CAAGACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA 6001 GAAGCTGCGC GGCGGAGTGG TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT 6061 GGACGCCCGC AAGATCCGCG AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT 6121 GTAATAATTC TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA 6181 TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG 6241 TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 6301 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA 6361 AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT 6421 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 6481 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 6541 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 6601 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 6661 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 6721 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 6781 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 6841 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 6901 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 6961 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 7021 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 7081 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 7141 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 7201 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 7261 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 7321 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 7381 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 7441 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 7501 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 7561 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 7621 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 7681 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 7741 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 7801 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 7861 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 7921 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 7981 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 8041 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 8101 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 8161 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.