pCMV-HSPA8-6×His-Neo plasmid

pCMV-HSPA8-6×His-Neo plasmid

PVT86197

pCMV-HSPA8-6×His-Neo plasmid Information

Plasmid name: pCMV-HSPA8(human)-6×His-Neo
Alias: NM_006597.6;HEL-33; HEL-S-72p; HSC54; HSC70; HSC71;HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71
Gene lenght: 1938bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCDNA3.1-MCS-6×His
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-HSPA8-6×His-Neo plasmid Description

pCMV-HSPA8-6×His-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and CMV promoter that can jointly initiate the transcription of the human HSPA8 gene, promoting the C-terminal fusion expression of a 6×His tag for facilitating the affinity interaction of the fusion protein with nickel ions for protein purification, and can also use anti-His antibodies to detect and identify the target protein. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-HSPA8-6×His-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCATG TCCAAGGGAC CTGCAGTTGG TATTGATCTT GGCACCACCT ACTCTTGTGT 961 GGGTGTTTTC CAGCACGGAA AAGTCGAGAT AATTGCCAAT GATCAGGGAA ACCGAACCAC 1021 TCCAAGCTAT GTCGCCTTTA CGGACACTGA ACGGTTGATC GGTGATGCCG CAAAGAATCA 1081 AGTTGCAATG AACCCCACCA ACACAGTTTT TGATGCCAAA CGTCTGATTG GACGCAGATT 1141 TGATGATGCT GTTGTCCAGT CTGATATGAA ACATTGGCCC TTTATGGTGG TGAATGATGC 1201 TGGCAGGCCC AAGGTCCAAG TAGAATACAA GGGAGAGACC AAAAGCTTCT ATCCAGAGGA 1261 GGTGTCTTCT ATGGTTCTGA CAAAGATGAA GGAAATTGCA GAAGCCTACC TTGGGAAGAC 1321 TGTTACCAAT GCTGTGGTCA CAGTGCCAGC TTACTTTAAT GACTCTCAGC GTCAGGCTAC 1381 CAAAGATGCT GGAACTATTG CTGGTCTCAA TGTACTTAGA ATTATTAATG AGCCAACTGC 1441 TGCTGCTATT GCTTACGGCT TAGACAAAAA GGTTGGAGCA GAAAGAAACG TGCTCATCTT 1501 TGACCTGGGA GGTGGCACTT TTGATGTGTC AATCCTCACT ATTGAGGATG GAATCTTTGA 1561 GGTCAAGTCT ACAGCTGGAG ACACCCACTT GGGTGGAGAA GATTTTGACA ACCGAATGGT 1621 CAACCATTTT ATTGCTGAGT TTAAGCGCAA GCATAAGAAG GACATCAGTG AGAACAAGAG 1681 AGCTGTAAGA CGCCTCCGTA CTGCTTGTGA ACGTGCTAAG CGTACCCTCT CTTCCAGCAC 1741 CCAGGCCAGT ATTGAGATCG ATTCTCTCTA TGAAGGAATC GACTTCTATA CCTCCATTAC 1801 CCGTGCCCGA TTTGAAGAAC TGAATGCTGA CCTGTTCCGT GGCACCCTGG ACCCAGTAGA 1861 GAAAGCCCTT CGAGATGCCA AACTAGACAA GTCACAGATT CATGATATTG TCCTGGTTGG 1921 TGGTTCTACT CGTATCCCCA AGATTCAGAA GCTTCTCCAA GACTTCTTCA ATGGAAAAGA 1981 ACTGAATAAG AGCATCAACC CTGATGAAGC TGTTGCTTAT GGTGCAGCTG TCCAGGCAGC 2041 CATCTTGTCT GGAGACAAGT CTGAGAATGT TCAAGATTTG CTGCTCTTGG ATGTCACTCC 2101 TCTTTCCCTT GGTATTGAAA CTGCTGGTGG AGTCATGACT GTCCTCATCA AGCGTAATAC 2161 CACCATTCCT ACCAAGCAGA CACAGACCTT CACTACCTAT TCTGACAACC AGCCTGGTGT 2221 GCTTATTCAG GTTTATGAAG GCGAGCGTGC CATGACAAAG GATAACAACC TGCTTGGCAA 2281 GTTTGAACTC ACAGGCATAC CTCCTGCACC CCGAGGTGTT CCTCAGATTG AAGTCACTTT 2341 TGACATTGAT GCCAATGGTA TACTCAATGT CTCTGCTGTG GACAAGAGTA CGGGAAAAGA 2401 GAACAAGATT ACTATCACTA ATGACAAGGG CCGTTTGAGC AAGGAAGACA TTGAACGTAT 2461 GGTCCAGGAA GCTGAGAAGT ACAAAGCTGA AGATGAGAAG CAGAGGGACA AGGTGTCATC 2521 CAAGAATTCA CTTGAGTCCT ATGCCTTCAA CATGAAAGCA ACTGTTGAAG ATGAGAAACT 2581 TCAAGGCAAG ATTAACGATG AGGACAAACA GAAGATTCTG GACAAGTGTA ATGAAATTAT 2641 CAACTGGCTT GATAAGAATC AGACTGCTGA GAAGGAAGAA TTTGAACATC AACAGAAAGA 2701 GCTGGAGAAA GTTTGCAACC CCATCATCAC CAAGCTGTAC CAGAGTGCAG GAGGCATGCC 2761 AGGAGGAATG CCTGGGGGAT TTCCTGGTGG TGGAGCTCCT CCCTCTGGTG GTGCTTCCTC 2821 AGGGCCCACC ATTGAAGAGG TTGATGCGGC CGCACATCAC CATCATCACC ATTGACTCGA 2881 GTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 2941 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 3001 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 3061 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 3121 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 3181 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 3241 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 3301 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 3361 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 3421 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 3481 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 3541 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 3601 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 3661 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 3721 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 3781 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 3841 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 3901 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 3961 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 4021 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 4081 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 4141 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 4201 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 4261 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 4321 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 4381 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 4441 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 4501 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 4561 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 4621 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 4681 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 4741 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 4801 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 4861 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 4921 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 4981 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 5041 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 5101 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 5161 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 5221 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 5281 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 5341 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 5401 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 5461 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 5521 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 5581 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 5641 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 5701 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 5761 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 5821 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 5881 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 5941 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 6001 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 6061 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 6121 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 6181 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 6241 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 6301 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 6361 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 6421 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 6481 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 6541 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 6601 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 6661 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 6721 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 6781 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 6841 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 6901 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 6961 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 7021 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 7081 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 7141 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 7201 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 7261 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG 7321 ACGGATCGGG AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.