pCMV-USP33-3×Myc-Neo plasmid

pCMV-USP33-3×Myc-Neo plasmid

PVT85922

pCMV-USP33-3×Myc-Neo plasmid Information

Plasmid name: pCMV-USP33(human)-3×Myc-Neo
Alias: NM_201626.3;VDU1;
Gene lenght: 2484bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-3×Myc-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-USP33-3×Myc-Neo plasmid Description

pCMV-USP33-3×Myc-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the human USP33 gene, promoting the C-terminal fusion expression of the USP33 gene with a 3×Myc tag, which facilitates the identification and tracking of the Myc-tagged target protein in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level, subcellular localization, and interactions with other proteins of the target protein. The plasmid carries a Neo/G418 resistance gene for the selection of host cells expressing the target gene.

pCMV-USP33-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGAC AGGATCAAAT 721 TCACACATAA CGATATTAAC CTTAAAGGTG TTACCTCATT TTGAAAGTCT TGGGAAACAG 781 GAAAAAATTC CTAACAAAAT GTCAGCTTTT CGAAATCATT GTCCACATTT GGATTCAGTT 841 GGTGAAATAA CAAAAGAAGA TTTGATACAA AAATCCCTTG GTACTTGTCA GGATTGTAAA 901 GTCCAAGGAC CAAATCTTTG GGCATGTCTG GAGAATAGAT GTTCATATGT TGGCTGTGGT 961 GAATCACAAG TAGATCACAG CACCATACAT TCTCAGGAGA CAAAGCATTA TCTAACTGTG 1021 AACCTTACCA CTCTTCGAGT ATGGTGTTAT GCTTGCAGCA AAGAAGTATT TTTGGATAGG 1081 AAATTAGGAA CTCAGCCTTC ATTGCCTCAT GTAAGACAAC CTCACCAAAT ACAAGAAAAC 1141 AGTGTCCAGG ATTTTAAAAT ACCCAGTAAT ACAACATTAA AAACTCCTCT GGTTGCCGTA 1201 TTTGATGATC TGGATATAGA AGCGGATGAA GAAGATGAAC TTAGGGCCAG AGGTCTTACA 1261 GGTTTGAAAA ATATTGGAAA TACTTGTTAC ATGAATGCAG CTTTGCAGGC TCTTTCTAAT 1321 TGCCCACCTT TGACACAGTT TTTTCTTGAT TGTGGAGGAC TAGCTCGAAC AGATAAGAAA 1381 CCTGCCATTT GTAAAAGTTA TCTCAAACTA ATGACAGAGC TGTGGCATAA AAGCAGGCCA 1441 GGATCTGTTG TGCCTACTAC TCTGTTTCAA GGAATTAAAA CTGTAAATCC AACATTTCGG 1501 GGGTATTCTC AGCAGGATGC TCAAGAATTC CTTCGATGTT TAATGGATTT GCTTCATGAA 1561 GAATTGAAAG AGCAAGTCAT GGAAGTAGAA GAAGATCCGC AAACCATAAC CACTGAGGAG 1621 ACAATGGAAG AAGACAAGAG CCAGTCGGAT GTAGATTTTC AGTCTTGTGA ATCTTGTAGC 1681 AACAGTGATA GAGCAGAAAA TGAAAATGGC TCTAGATGCT TTTCTGAAGA TAATAATGAA 1741 ACAACAATGT TAATTCAGGA TGATGAAAAC AATTCAGAAA TGTCAAAGGA TTGGCAAAAA 1801 GAGAAGATGT GCAATAAGAT TAATAAAGTA AATTCTGAAG GCGAATTTGA TAAAGATAGA 1861 GACTCTATAT CTGAAACAGT CGACTTAAAC AACCAGGAAA CTGTCAAAGT GCAAATACAC 1921 AGCAGAGCTT CAGAATATAT CACTGATGTC CATTCGAATG ACCTGTCTAC ACCACAGATC 1981 CTTCCATCAA ATGAAGGTGT TAATCCACGT TTATCGGCAA GCCCTCCTAA ATCAGGCAAT 2041 TTGTGGCCAG GATTGGCACC ACCACACAAA AAAGCTCAGT CTGCATCTCC AAAGAGAAAA 2101 AAACAGCACA AGAAATACAG AAGTGTTATT TCAGACATAT TTGATGGAAC AATCATTAGT 2161 TCAGTGCAGT GTCTGACTTG TGACAGGGTG TCTGTAACCC TCGAGACCTT TCAAGATCTG 2221 TCCTTGCCAA TTCCTGGCAA GGAAGACCTT GCTAAGCTGC ATTCATCAAG TCATCCAACT 2281 TCTATAGTCA AAGCAGGATC ATGTGGCGAA GCATATGCTC CACAAGGGTG GATAGCTTTT 2341 TTCATGGAAT ATGTGAAGAG CTGGTTTTGG GGTCCAGTAG TAACCTTGCA AGATTGTCTT 2401 GCTGCCTTCT TTGCCAGAGA TGAACTAAAA GGTGACAATA TGTACAGTTG TGAAAAATGC 2461 AAAAAGTTGA GAAATGGAGT GAAGTTTTGT AAAGTACAAA ACTTTCCTGA GATTTTGTGC 2521 ATCCACCTTA AAAGATTCAG ACATGAACTA ATGTTTTCCA CCAAAATCAG TACCCATGTT 2581 TCATTTCCGC TAGAAGGCTT GGATCTTCAG CCATTTCTTG CTAAGGATAG TCCAGCTCAA 2641 ATTGTGACAT ATGATCTTCT GTCAGTCATT TGCCATCATG GAACTGCAAG TAGTGGACAC 2701 TATATAGCCT ACTGCCGAAA CAATCTAAAT AATCTCTGGT ATGAATTTGA TGATCAGAGT 2761 GTCACTGAAG TTTCAGAATC TACTGTACAA AATGCAGAAG CTTACGTTCT TTTCTATAGG 2821 AAGAGCAGCG AAGAGGCACA AAAAGAGAGG AGAAGGATAT CAAATTTATT GAACATAATG 2881 GAACCAAGCC TCCTTCAGTT TTATATTTCT CGACAGTGGC TTAATAAATT TAAGACCTTT 2941 GCCGAACCTG GCCCTATTTC AAATAATGAC TTTCTTTGTA TTCATGGAGG TGTTCCTCCA 3001 AGAAAAGCTG GTTATATTGA AGACCTGGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 3061 CTATATAGCA GGTATGGTGG AGGACCAGCT GTCAACCATC TGTACATTTG TCATACTTGC 3121 CAAATTGAGG CGGAGAAAAT TGAAAAAAGA AGAAAAACTG AATTGGAAAT TTTTATTCGG 3181 GTAAAAAAGC TCGAGGAACA AAAACTAATA TCAGAGGAAG ATTTGGAACA GAAACTAATC 3241 AGTGAAGAAG ATTTAGAGCA AAAGTTAATT TCCGAGGAGG ACTTATAGTG AGGGCCCGAT 3301 ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 3361 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 3421 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 3481 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 3541 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 3601 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 3661 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT 3721 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 3781 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 3841 CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG 3901 GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT 3961 GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG 4021 GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT 4081 ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC 4141 TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA 4201 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 4261 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 4321 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG 4381 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 4441 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 4501 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 4561 CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 4621 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC 4681 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG 4741 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 4801 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 4861 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 4921 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 4981 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 5041 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 5101 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 5161 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 5221 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 5281 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 5341 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 5401 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 5461 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 5521 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 5581 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 5641 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 5701 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 5761 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 5821 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG 5881 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 5941 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 6001 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 6061 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 6121 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 6181 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 6241 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 6301 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 6361 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 6421 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 6481 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 6541 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 6601 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 6661 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 6721 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 6781 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 6841 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 6901 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 6961 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 7021 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 7081 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 7141 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 7201 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 7261 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 7321 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 7381 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 7441 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 7501 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 7561 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 7621 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 7681 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 7741 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 7801 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 7861 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 7921 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 7981 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 8041 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 8101 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 8161 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 8221 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 8281 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 8341 CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG 8401 CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA 8461 GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA 8521 GAATCTGCTT AGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.