pUC57-VanD(faecium) plasmid

pUC57-VanD(faecium) plasmid

PVT86263

pUC57-VanD(faecium) plasmid Information

Plasmid name: pUC57-VanD(faecium)
Alias: -
Gene lenght: 1032bp
Host: E.coli
Use(s): Gene Template
Fragment type: cDNA
Species: Enterococcus faecium
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter:
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pUC57
Forward primer: pQE30-F
Reverse primer:
Copy number: High
Induction:

pUC57-VanD(faecium) plasmid Description

pUC57-VanD(faecium) This plasmid is suitable for use in Escherichia coli hosts, serving to replicate and produce DNA that preserves the VanD gene template.

pUC57-VanD(faecium) plasmid Sequence

1 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 61 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 1141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 1201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 1261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 1321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 1381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 1441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 1501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 1561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 1621 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 1681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 1741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 1801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 1861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 1921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 1981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 2041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 2101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 2161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 2221 ACCATGATTA CGCCAAGCTT GCATGCAGGC CTCTGCAGTC GACGGGCCCG GGATCCATGT 2281 ATAAGCTTAA AATTGCAGTC CTGTTTGGAG GCTGCTCAGA GGAACATGAT GTTTCAGTGA 2341 AATCTGCGAT GGAGGTTGCA GCAAATATAA ACAAGGAAAA ATACCAGCCG TTTTATATTG 2401 GAATCACAAA ATCCGGCGCA TGGAAACTAT GCGATAAGCC CTGCCGGGAC TGGGAAAACT 2461 ATGCGGGATA CCCGGCTGTG ATTTCTCCGG ACAGAAGGAC CCATGGCCTG CTGATACAAA 2521 AGGATGGCGG ATATGAGAGC CAGCCTGTAG ACGTGGTGCT TCCGATGATT CATGGAAAAT 2581 TTGGCGAGGA CGGAACCATA CAGGGTCTGC TTGAGCTGTC CGGCATTCCT TATGTGGGAT 2641 GCGACATTCA AAGTTCTGTA ACCTGTATGG ATAAGTCGCT CGCTTATATG GTTGTGAAAA 2701 ATGCGGGAAT TGAGGTACCT GGGTTTCGAG TTCTACAAAA GGGGGACAGC CTGAAAGCAG 2761 AGACACTCTC GTACCCGGTC TTTGTAAAAC CTGCCCGTTC CGGCTCCTCT TTTGGCGTGA 2821 ATAAGGTATG CCGGGCAGAG GAACTGCAGG CAGCGGTCAC AGAGGCGGGC AAGTATGACT 2881 GCAAAATATT GGTTGAGGAG GCCGTTTCCG GGAGTGAGGT AGGCTGTGCC ATACTGGGAA 2941 ATGAAAATGC TCTCATGGCT GGCGAGGTGG ATCAGATTGA GCTGAGACAC GGTTTTTTTA 3001 AGATTCATCA GGAAGCACAG CCGGAGAAAG GTTCTGAAAA TGCAGTTATC AGAGTTCCAG 3061 CTGTCTTACC GGATGAGGTA AGAGAACGGA TTCAGAAAAC AGCAATGAAG ATTTACCGGA 3121 TACTTGGCTG CCGAGGATTG GCTCGTATTG ATCTGTTTTT GCGGGAGGAC GGCTGCATTG 3181 TGCTGAATGA AGTGAATACC ATGCCGGGTT TTACTTCCTA CAGCCGTTAT CCCCGCATGA 3241 TGACAGCAGC CGGTTTTACG CTTACTGAAA TACTGGATCG CTTGATTGAA CTTTCACTTA 3301 GGAGGTAAGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT 3361 ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG 3421 GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG 3481 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT 3541 ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC 3601 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC 3661 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.