pCMV-Bcl11b-3×FLAG-Neo plasmid

pCMV-Bcl11b-3×FLAG-Neo plasmid

PVT86680

pCMV-Bcl11b-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Bcl11b(mouse)-3×FLAG-Neo
Alias: NM_001079883.1;9130430L19Rik; B630002E05Rik; BCL-11B;Ctip2; Rit1
Gene lenght: 2652bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-MCS-3×FLAG-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Bcl11b-3×FLAG-Neo plasmid Description

pCMV-Bcl11b-3×FLAG-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate efficient expression of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-Bcl11b-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCATGTCCC GCCGCAAACA GGGCAACCCG CAGCACTTGT CCCAGAGGGA ACTCATCACG 1021 CCAGAGGCTG ACCATGTGGA GGCTACCATC CTCGAGGAAG ACGAGGGTCT GGAGATAGAG 1081 GAGCCTAGCA GCCTGGGGCT GATGGTGGGA GGCCCCGACC CTGATCTACT CACCTGTGGC 1141 CAGTGTCAGA TGAACTTCCC GCTGGGGGAC ATCCTGGTTT TTATAGAGCA CAAGAAGAAA 1201 CAGTGTGGAG GCCTGGGCCC CTGCTACGAC AAGGTCCTGG ACAAGAGCAG TCCACCTCCC 1261 TCCTCTCGCT CTGAGCTCAG GAGAGTATCT GAGCCAGTGG AGATCGGGAT CCAGGTCACC 1321 CCTGATGAAG ATGACCACCT ACTGTCACCC ACGAAAGGCA TCTGTCCCAA GCAGGAGAAC 1381 ATTGCAGGGC CGTGCAGGCC TGCCCAGCTG CCATCGATGG CCCCCATAGC TGCCTCCTCT 1441 TCCCACCCTC CCACCTCCGT GATTACTTCA CCTCTGCGTG CCCTGGGCGT TCTCCCACCC 1501 TGTTTCCCGC TGCCTTGCTG TGGTGCACGC CCCATCTCGG GCGACGGGAC TCAGGGTGAG 1561 GGTCAGATGG AGGCTCCCTT TGGATGCCAG TGTGAGTTGT CAGGTAAAGA TGAGCCTTCC 1621 AGCTACATTT GCACAACATG CAAGCAGCCC TTCAACAGCG CCTGGTTCCT GCTGCAGCAC 1681 GCACAGAACA CACATGGCTT CCGAATCTAC CTGGAGCCTG GGCCGGCCAG CACCTCGCTC 1741 ACGCCCAGGC TCACCATCCC GCCACCGCTC GGGCCGGAGA CCGTGGCGCA GTCCCCACTC 1801 ATGAATTTCC TGGGGGACAG CAATCCTTTC AACCTGCTGC GCATGACGGG CCCCATCCTG 1861 CGGGACCACC CTGGCTTCGG TGAGGGCCGC TTGCCAGGTA CGCCACCGCT CTTCAGCCCA 1921 CCGCCACGCC ATCACTTGGA CCCACACCGC CTCAGTGCAG AGGAGATGGG GCTCGTGGCC 1981 CAGCACCCCA GTGCCTTCGA CCGAGTCATG CGCCTGAACC CCATGGCCAT AGACTCTCCT 2041 GCCATGGACT TCTCCCGGCG GCTGCGAGAA CTGGCCGGCA ACAGCTCCAC GCCGCCGCCC 2101 GTGTCCCCAG GCCGTGGCAA CCCTATGCAC CGGCTGCTGA ACCCTTTCCA GCCCAGTCCC 2161 AAGTCCCCGT TCCTCAGCAC GCCACCGCTG CCACCCATGC CTGCGGGCAC ACCGCCACCG 2221 CAGCCGCCTG CCAAGAGCAA GTCCTGTGAG TTCTGCGGCA AGACCTTCAA GTTCCAGAGC 2281 AATCTCATCG TGCACCGGCG CAGCCACACG GGCGAGAAGC CCTACAAGTG CCAGCTGTGC 2341 GACCATGCGT GCTCGCAGGC GAGCAAGCTC AAGCGCCACA TGAAGACGCA CATGCACAAG 2401 GCGGGCTCTC TGGCTGGCCG CTCAGACGAC GGGCTCTCAG CTGCCAGCTC CCCTGAGCCG 2461 GGCACCAGCG AGCTGCCAGG TGACCTGAAA GCGGCCGATG GCGACTTCCG CCACCATGAG 2521 AGCGACCCAT CTCTGGGCCC CGAGCCTGAG GACGACGAGG ACGAGGAGGA GGAAGAAGAG 2581 GAGCTGCTGC TGGAGAACGA GAGCCGGCCT GAGTCGAGCT TCAGCATGGA CTCGGAGCTG 2641 GGCCGTGGCC GCGAGAACGG AGGTGGCGTG CCACCGGGGG TGGCGGGCGC AGGGGCTGCA 2701 GCTGCGGCTC TGGCGGATGA GAAGGCTCTG GCCCTGGGCA AGGTGATGGA GGACGCAGGG 2761 CTGGGCGCAC TGCCGCAGTA TGGGGAGAAG CGGGGCGCCT TCCTGAAGCG TGCAGGCGAC 2821 ACGGGTGATG CCGGAGCTGT TGGCTGTGGG GACGCGGGTG CACCGGGTGC AGTGAACGGG 2881 CGCGGCGGGG CCTTCGCGCC AGGCGCAGAG CCCTTTCCAG CTCTCTTCCC ACGCAAGCCA 2941 GCACCGCTGC CCAGCCCTGG GCTCGGTGGT CCCGCGCTGC ACGCGGCCAA GCGCATCAAG 3001 GTGGAGAAAG ACCTGGAGCT GCCACCTGCC GCCCTCATCC CATCTGAGAA CGTGTACTCG 3061 CAGTGGCTCG TGGGCTACGC AGCATCGCGC CACTTCATGA AGGACCCATT CCTGGGCTTC 3121 ACGGATGCGC GCCAGTCGCC TTTCGCCACA TCGTCGGAAC ATTCCTCTGA GAACGGCAGC 3181 CTGCGCTTCT CAACGCCACC CGGGGACCTG CTGGACGGCG GGCTGTCCGG GCGCAGTGGC 3241 ACGGCGAGCG GGGGCAGCAC ACCTCACCTG GGTGGTCCGG GTCCTGGGAG GCCGAGCTCC 3301 AAGGAGGGCC GCCGCAGCGA CACATGTGAG TACTGCGGCA AGGTCTTCAA GAACTGTAGC 3361 AACCTGACGG TGCACCGGAG GAGCCACACC GGCGAGCGGC CTTACAAGTG CGAGCTGTGC 3421 AACTACGCGT GCGCGCAGAG CAGCAAGCTC ACGCGCCACA TGAAGACGCA CGGGCAGATC 3481 GGCAAGGAGG TGTACCGCTG CGACATCTGC CAGATGCCCT TCAGCGTCTA CAGCACCCTG 3541 GAGAAACACA TGAAAAAGTG GCACGGTGAA CACTTGCTGA CTAATGATGT CAAAATCGAG 3601 CAGGCTGAGA GGAGCCTCGA GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3661 ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG TTTAAACCCG CTGATCAGCC 3721 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 3781 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 3841 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 3901 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 3961 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 4021 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 4081 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 4141 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 4201 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 4261 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 4321 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 4381 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT 4441 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 4501 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 4561 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 4621 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 4681 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 4741 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 4801 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 4861 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 4921 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 4981 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 5041 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 5101 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 5161 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA 5221 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 5281 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 5341 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG 5401 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 5461 GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG 5521 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG 5581 ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 5641 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 5701 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 5761 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 5821 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 5881 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 5941 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 6001 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 6061 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 6121 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 6181 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 6241 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 6301 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 6361 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 6421 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 6481 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 6541 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 6601 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 6661 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 6721 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 6781 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 6841 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 6901 TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 7081 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 7141 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 7201 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 7261 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 7321 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 7381 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 7441 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 7501 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 7561 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 7621 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 7681 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 7741 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 7801 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 7861 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 7921 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 7981 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 8041 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 8101 TCCCCGAAAA GTGCCACCTG ACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.