pCMV-Pfkl-3×FLAG-Neo plasmid

pCMV-Pfkl-3×FLAG-Neo plasmid

PVT86210

pCMV-Pfkl-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Pfkl(mouse)-3×FLAG-Neo
Alias: NM_008826.5;ATP-PFK; PFK-B; PFK-L;
Gene lenght: 2340bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-MCS-3×FLAG-WPRE-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Pfkl-3×FLAG-Neo plasmid Description

pCMV-Pfkl-3×FLAG-Neo This is a plasmid suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the mouse Pfkl gene, promoting the C-terminal fusion expression of the PIK3CG gene with a 3×FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The WPRE protein enhances the processing and stability of the target gene after transcription, helping to increase the half-life and translation efficiency of the mRNA. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-Pfkl-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGC TACCGTGGAC CTGGAGAAAC 781 TGCGGATGTC GGGGGCTGGC AAGGCCATTG GAGTGCTGAC CAGCGGCGGT GATGCGCAAG 841 GTATGAATGC TGCTGTCAGG GCTGTGACCC GTATGGGCAT ATATGTGGGG GCCAAAGTCT 901 TCCTCATCTA TGAGGGCTAC GAGGGCCTTG TGGAAGGAGG CGAGAACATC AAGCCAGCCA 961 ACTGGCTCAG CGTTTCCAAT ATCATCCAGC TGGGTGGCAC CATTATTGGC AGTGCCCGCT 1021 GTAAGGCCTT CACTACGAGG GAAGGCCGCC TGGCTGCAGC CTACAATCTG CTCCAACACG 1081 GCATCACCAA CCTGTGTGTC ATCGGTGGCG ACGGCAGCCT CACGGGGGCC AACATCTTCC 1141 GCAACGAGTG GGGCAGCTTG CTGGAGGAGC TGGTGAAGGA AGGCAAGATC TCAGAGTCCA 1201 CAGCTCAGAA CTACGCACAC TTGACCATCG CCGGTCTGGT GGGCTCCATC GATAACGACT 1261 TCTGTGGCAC TGACATGACC ATTGGCACAG ACTCAGCCCT GCACCGCATT ATGGAGGTCA 1321 TTGACGCCAT CACTACCACT GCCCAAAGTC ACCAGAGGAC CTTTGTTTTG GAGGTGATGG 1381 GACGGCACTG CGGGTACCTG GCGCTGGTGT CTGCCCTGGC TTCCGGGGCT GATTGGCTAT 1441 TCATTCCTGA AGCGCCCCCT GAGGATGGCT GGGAGAACTT CATGTGTGAG AGGCTGGGCG 1501 AGACTCGGAG CCGAGGCTCT CGGCTGAACA TCATCATCAT CGCAGAGGGT GCCATTGACC 1561 GGCATGGAAA GCCTATCTCA TCCAGCTACG TGAAGGATCT GGTGGTTCAG AGGCTGGGCT 1621 TCGATACACG AGTGACTGTG CTGGGTCATG TACAGCGAGG AGGGACGCCT TCAGCCTTCG 1681 ACCGAATCCT GAGTAGCAAG ATGGGTATGG AGGCCGTGAT GGCGCTGCTA GAGGCCACGC 1741 CTGACACGCC GGCCTGTGTG GTCAGCCTCT CCGGGAACCA GTCTGTGAGG CTACCTCTCA 1801 TGGAGTGTGT GCAAGTGACA AAGGACGTGC AGAAGGCCAT GGACGAGGAG AGGTTTGACG 1861 AGGCCATCCA GCTCCGTGGC AGGAGCTTTG AGAACAACTG GAAAATTTAC AAGCTCCTTG 1921 CCCACCAGAA GGTGTCTAAA GAGAAGTCCA ACTTCTCCCT GGCCATCCTG AATGTGGGGG 1981 CTCCAGCTGC TGGCATGAAT GCAGCTGTGC GCTCCGCAGT GCGCACTGGT ATCTCCGAGG 2041 GACACACGGT ATACATCGTG CATGATGGCT TTGAGGGTCT GGCCAAGGGT CAGGTGCAAG 2101 AAGTGGGCTG GCATGATGTG GCAGGCTGGC TGGGACGTGG TGGCTCGATG CTGGGGACCA 2161 AGAGGACACT GCCCAAGCCC CACCTGGAGG CCATTGTGGA GAATCTCCGC ACCTACAACA 2221 TCCACGCCCT GCTGGTGATT GGTGGCTTTG AGGCCTACGA GGGTGTGCTG CAGCTGGTGG 2281 AGGCCCGGGG GCGCTACGAG GAACTCTGCA TTGTCATGTG TGTCATCCCA GCCACCATCA 2341 GCAACAATGT GCCTGGCACT GACTTCAGCC TGGGCTCAGA CACGGCTGTC AACGCTGCAA 2401 TGGAGAGTTG TGATCGCATC AAGCAGTCAG CCTCAGGGAC AAAGCGGCGT GTGTTCATTG 2461 TAGAGACCAT GGGGGGCTAC TGTGGCTACC TGGCTACTGT GACCGGCATT GCTGTGGGTG 2521 CCGATGCTGC CTACGTCTTT GAGGACCCTT TCAACATCCA TGACTTAAAG GCCAATGTGG 2581 AGCATATGAC AGAGAAGATG AAGACAGACA TCCAGAGGGG ACTGGTGCTC CGGAACGAGA 2641 AGTGTCACGA ACACTACACC ACAGAGTTCC TATACAACCT GTACTCCTCA GAAGGCAGGG 2701 GCGTGTTTGA CTGCAGGACC AATGTGCTGG GCCACTTGCA GCAGGGTGGT GCTCCAACCC 2761 CTTTCGACCG GAACTATGGG ACCAAACTGG GGGTGAAGGC CATGTTGTGG GTGTCTGAGA 2821 AGCTACGTGA TGTCTACCGT AAAGGGCGGG TGTTTGCCAA TGCTCCAGAC TCAGCCTGTG 2881 TGATCGGCCT GCGGAAGAAG GTAGTGGCCT TCAGTCCGGT CACAGAACTC AAGAAAGAGA 2941 CTGATTTTGA GCACCGCATG CCCCGGGAGC AGTGGTGGCT GAATCTGCGG CTGATGCTGA 3001 AGATGCTGGC ACACTACCGC ATCAGCATGG CAGACTATGT GTCTGGGGAG CTGGAGCACG 3061 TCACACGCCG CACCTTGAGC ATAGACAAGG GTTTCGGATC CGACTACAAA GACCATGACG 3121 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG 3181 ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 3241 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 3301 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 3361 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 3421 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 3481 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 3541 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC 3601 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 3661 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 3721 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 3781 TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT 3841 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG 3901 CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA 3961 ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG 4021 GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC 4081 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 4141 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 4201 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 4261 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 4321 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 4381 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 4441 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 4501 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 4561 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 4621 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 4681 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 4741 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 4801 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 4861 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 4921 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 4981 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 5041 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 5101 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 5161 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 5221 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 5281 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 5341 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 5401 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 5461 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 5521 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 5581 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 5641 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 5701 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA 5761 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 5821 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 5881 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 5941 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 6001 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 6061 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 6121 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 6181 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 6241 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 6301 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 6361 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 6421 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 6481 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 6541 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 6601 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 6661 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 6721 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 6781 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 6841 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 6901 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 6961 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 7021 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 7081 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 7141 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 7201 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 7261 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 7321 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 7381 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 7441 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 7501 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 7561 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 7621 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 7681 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 7741 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 7801 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 7861 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 7921 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 7981 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 8041 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 8101 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 8161 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 8221 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 8281 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT 8341 GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG 8401 AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.