pRetroX-Tbx21-mCherry-Puro pla

pRetroX-Tbx21-mCherry-Puro plasmid

PVT86715

pRetroX-Tbx21-mCherry-Puro plasmid Information

Plasmid name: pRetroX-Tbx21(mouse)-mCherry-Puro
Alias: NM_019507.2;Tbet; Tblym; TBT1
Gene lenght: 1593bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pECMV-Tbx21-m-FLAG
Forward primer: pRetroX-F1：TGGTTCATTCTCAAGCCTCAGA
Reverse primer:
Copy number: High
Induction:

pRetroX-Tbx21-mCherry-Puro plasmid Description

pRetroX-Tbx21-mCherry-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and CMV promoter that can jointly initiate the efficient expression of downstream genes, and it carries a red fluorescent protein at the C-terminus of the gene, allowing for visual observation of the location and level of target gene expression. The plasmid also carries a Puro resistance gene for selecting host cells that express the target gene.

pRetroX-Tbx21-mCherry-Puro plasmid Sequence

1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT 61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG 121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG 181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT 241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA 301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG 361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC 421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGTCTAGAG ATATACTGAG TCATTAGGGA 481 CTTTCCAATG GGTTTTGCCC AGTACATAAG GTCAATAGGG GTGAATCAAC AGGAAAGTCC 541 CATTGGAGCC AAGTACACTG AGTCAATAGG GACTTTCCAT TGGGTTTTGC CCAGTACAAA 601 AGGTCAATAG GGGGTGAGTC AATGGGTTTT TCCCATTATT GGCACGTACA TAAGGTCAAT 661 AGGGGTGAGT CATTGGGTTT TTCCAGCCAA TTTAATTAAA ACGCCATGTA CTTTCCCACC 721 ATTGACGTCA ATGGGCTATT GAAACTAATG CAACGTGACC TTTAAACGGT ACTTTCCCAT 781 AGCTGATTAA TGGGAAAGTA CCGTTCTCGA GCCAATACAC GTCAATGGGA AGTGAAAGGG 841 CAGCCAAAAC GTAACACCGC CCCGGTTTTC CCCTGGAAAT TCCATATTGG CACGCATTCT 901 ATTGGCTGAG CTGCGTTCTA CGTGGGTATA AGAGGCGCGA CCAGCGTCGG TACCGTCGCA 961 GTCTTCGGTC TGACCACCGT AGAACGCAGA TCGAATTCAT GGTGAGCAAG GGCGAGGAGC 1021 TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT 1081 TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA 1141 TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG 1201 GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG 1261 CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA 1321 AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG 1381 GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA 1441 GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA 1501 TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC 1561 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC 1621 TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG 1681 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT CGAGATCCAC 1741 CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT 1801 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT 1861 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 1921 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 1981 TTAACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA 2041 GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 2101 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 2161 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG 2221 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 2281 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 2341 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2401 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2461 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2521 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCTCTGG 2581 CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG 2641 ACCCAAGCTG GCTAGCGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG 2701 ATATCGCCAC CATGAGCACA GGTGATAGTT TCGAGACAAG ATTCGAGAAA ATGGATAATC 2761 TGCTGAGGGA CCCCAAAAGT GAAGTTAACT CCGATTGCCT GCTTGATGGT CTGGACGCCC 2821 TTGTCTATGA CTTGGACTTC CCCGCCCTTC GCAAAAACAA GAACATTGAC AACTTCCTGT 2881 CTAGGTATAA GGACACCATC AACAAGATAA GGGATCTCCG CATGAAGGCC GAAGATTACG 2941 AGGTGGTTAA GGTGATCGGA CGCGGTGCTT TTGGAGAGGT GCAGCTTGTG CGGCACAAAT 3001 CAACAAGGAA GGTTTACGCG ATGAAGCTTC TCAGCAAATT TGAAATGATA AAGAGATCTG 3061 ATTCTGCTTT TTTCTGGGAA GAAAGGGACA TCATGGCTTT TGCCAACAGT CCTTGGGTTG 3121 TTCAGCTTTT TTATGCATTC CAAGATGATC GTTATCTCTA CATGGTGATG GAATACATGC 3181 CTGGTGGAGA TCTTGTAAAC TTAATGAGCA ACTATGATGT GCCTGAAAAA TGGGCACGAT 3241 TCTATACTGC AGAAGTAGTT CTTGCATTGG ATGCAATCCA TTCCATGGGT TTTATTCACA 3301 GAGATGTGAA GCCTGATAAC ATGCTGCTGG ATAAATCTGG ACATTTGAAG TTAGCAGATT 3361 TTGGTACTTG TATGAAGATG AATAAGGAAG GCATGGTACG ATGTGATACA GCGGTTGGAA 3421 CACCTGATTA TATTTCCCCT GAAGTATTAA AATCCCAAGG TGGTGATGGT TATTATGGAA 3481 GAGAATGTGA CTGGTGGTCG GTTGGGGTAT TTTTATACGA AATGCTTGTA GGTGATACAC 3541 CTTTTTATGC AGATTCTTTG GTTGGAACTT ACAGTAAAAT TATGAACCAT AAAAATTCAC 3601 TTACCTTTCC TGATGATAAT GACATATCAA AAGAAGCAAA AAACCTTATT TGTGCCTTCC 3661 TTACTGACAG GGAAGTGAGG TTAGGGCGAA ATGGTGTAGA AGAAATCAAA CGACATCTCT 3721 TCTTCAAAAA TGACCAGTGG GCTTGGGAAA CGCTCCGAGA CACTGTAGCA CCAGTTGTAC 3781 CCGATTTAAG TAGTGACATT GATACTAGTA ATTTTGATGA CTTGGAAGAA GATAAAGGAG 3841 AGGAAGAAAC ATTCCCTATT CCTAAAGCTT TCGTTGGCAA TCAACTACCT TTTGTAGGAT 3901 TTACATATTA TAGCAATCGT AGATACTTAT CTTCAGCAAA TCCTAATGAT AACAGAACTA 3961 GCTCCAATGC AGATAAAAGC TTGCAGGAAA GTTTGCAAAA AACAATCTAT AAGCTGGAAG 4021 AACAGCTGCA TAATGAAATG CAGTTAAAAG ATGAAATGGA GCAGAAGTGC AGAACCTCAA 4081 ACATAAAACT AGACAAGATA ATGAAAGAAT TGGATGAAGA GGGAAATCAA AGAAGAAATC 4141 TAGAATCTAC AGTGTCTCAG ATTGAGAAGG AGAAAATGTT GCTACAGCAT AGAATTAATG 4201 AGTACCAAAG AAAAGCTGAA CAGGAAAATG AGAAGAGAAG AAATGTAGAA AATGAAGTTT 4261 CTACATTAAA GGATCAGTTG GAAGACTTAA AGAAAGTCAG TCAGAATTCA CAGCTTGCTA 4321 ATGAGAAGCT GTCCCAGTTA CAAAAGCAGC TAGAAGAAGC CAATGACTTA CTTAGGACAG 4381 AATCGGACAC AGCTGTAAGA TTGAGGAAGA GTCACACAGA GATGAGCAAG TCAATTAGTC 4441 AGTTAGAGTC CCTGAACAGA GAGTTGCAAG AGAGAAATCG AATTTTAGAG AATTCTAAGT 4501 CACAAACAGA CAAAGATTAT TACCAGCTGC AAGCTATATT AGAAGCTGAA CGAAGAGACA 4561 GAGGTCATGA TTCTGAGATG ATTGGAGACC TTCAAGCTCG AATTACATCT TTACAAGAGG 4621 AGGTGAAGCA TCTCAAACAT AATCTCGAAA AAGTGGAAGG AGAAAGAAAA GAGGCTCAAG 4681 ACATGCTTAA TCACTCAGAA AAGGAAAAGA ATAATTTAGA GATAGATTTA AACTACAAAC 4741 TTAAATCATT ACAACAACGG TTAGAACAAG AGGTAAATGA ACACAAAGTA ACCAAAGCTC 4801 GTTTAACTGA CAAACATCAA TCTATTGAAG AGGCAAAGTC TGTGGCAATG TGTGAGATGG 4861 AAAAAAAGCT GAAAGAAGAA AGAGAAGCTC GAGAGAAGGC TGAAAATCGG GTTGTTCAGA 4921 TTGAGAAACA GTGTTCCATG CTAGACGTTG ATCTGAAGCA ATCTCAGCAG AAACTAGAAC 4981 ATTTGACTGG AAATAAAGAA AGGATGGAGG ATGAAGTTAA GAATCTAACC CTGCAACTGG 5041 AGCAGGAATC AAATAAGCGG CTGTTGTTAC AAAATGAATT GAAGACTCAA GCATTTGAGG 5101 CAGACAATTT AAAAGGTTTA GAAAAGCAGA TGAAACAGGA AATAAATACT TTATTGGAAG 5161 CAAAGAGATT ATTAGAATTT GAGTTAGCTC AGCTTACGAA ACAGTATAGA GGAAATGAAG 5221 GACAGATGCG GGAGCTACAA GATCAGCTTG AAGCTGAGCA ATATTTCTCG ACACTTTATA 5281 AAACCCAGGT AAAGGAACTT AAAGAAGAAA TTGAAGAAAA AAACAGAGAA AATTTAAAGA 5341 AAATACAGGA ACTACAAAAT GAAAAAGAAA CTCTTGCTAC TCAGTTGGAT CTAGCAGAAA 5401 CAAAAGCTGA GTCTGAGCAG TTGGCGCGAG GCCTTCTGGA AGAACAGTAT TTTGAATTGA 5461 CGCAAGAAAG CAAGAAAGCT GCTTCAAGAA ATAGACAAGA GATTACAGAT AAAGATCACA 5521 CTGTTAGTCG GCTTGAAGAA GCAAACAGCA TGCTAACCAA AGATATTGAA ATATTAAGAA 5581 GAGAGAATGA AGAGCTAACA GAGAAAATGA AGAAGGCAGA GGAAGAATAT AAACTGGAGA 5641 AGGAGGAGGA GATCAGTAAT CTTAAGGCTG CCTTTGAAAA GAATATCAAC ACTGAACGAA 5701 CCCTTAAAAC ACAGGCTGTT AACAAATTGG CAGAAATAAT GAATCGAAAA GATTTTAAAA 5761 TTGATAGAAA GAAAGCTAAT ACACAAGATT TGAGAAAGAA AGAAAAGGAA AATCGAAAGC 5821 TGCAACTGGA ACTCAACCAA GAAAGAGAGA AATTCAACCA GATGGTAGTG AAACATCAGA 5881 AGGAACTGAA TGACATGCAA GCGCAATTGG TAGAAGAATG TGCACATAGG AATGAGCTTC 5941 AGATGCAGTT GGCCAGCAAA GAGAGTGATA TTGAGCAATT GCGTGCTAAA CTTTTGGACC 6001 TCTCGGATTC TACAAGTGTT GCTAGTTTTC CTAGTGCTGA TGAAACTGAT GGTAACCTCC 6061 CAGAGTCAAG AATTGAAGGT TGGCTTTCAG TACCAAATAG AGGAAATATC AAACGATATG 6121 GCTGGAAGAA ACAGTATGTT GTGGTAAGCA GCAAAAAAAT TTTGTTCTAT AATGACGAAC 6181 AAGATAAGGA GCAATCCAAT CCATCTATGG TATTGGACAT AGATAAACTG TTTCACGTTA 6241 GACCTGTAAC CCAAGGAGAT GTGTATAGAG CTGAAACTGA AGAAATTCCT AAAATATTCC 6301 AGATACTATA TGCAAATGAA GGTGAATGTA GAAAAGATGT AGAGATGGAA CCAGTACAAC 6361 AAGCTGAAAA AACTAATTTC CAAAATCACA AAGGCCATGA GTTTATTCCT ACACTCTACC 6421 ACTTTCCTGC CAATTGTGAT GCCTGTGCCA AACCTCTCTG GCATGTTTTT AAGCCACCCC 6481 CTGCCCTAGA GTGTCGAAGA TGCCATGTTA AGTGCCACAG AGATCACTTA GATAAGAAAG 6541 AGGACTTAAT TTGTCCATGT AAAGTAAGTT ATGATGTAAC ATCAGCAAGA GATATGCTGC 6601 TGTTAGCATG TTCTCAGGAT GAACAAAAAA AATGGGTAAC TCATTTAGTA AAGAAAATCC 6661 CTAAGAATCC ACCATCTGGT TTTGTTCGTG CTTCCCCTCG AACGCTTTCT ACAAGATCCA 6721 CTGCAAATCA GTCTTTCCGG AAAGTGGTCA AAAATACATC TGGAAAAACT AGTTAAAAGC 6781 TTAAGTTTAA ACCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 6841 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 6901 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 6961 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 7021 GTGGGCTCTA TGGGTCGACT TCGAGCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 7081 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 7141 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CGTCTAGCAT CGAAGATCCA 7201 ATAACTTCGT ATAGCATACA TTATACGAAG TTATAAGTAG CTTGGCGTAA TCATGGTCAT 7261 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 7321 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 7381 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 7441 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 7501 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 7561 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 7621 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 7681 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 7741 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 7801 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC 7861 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 7921 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 7981 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 8041 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 8101 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 8161 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 8221 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 8281 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 8341 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 8401 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 8461 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 8521 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 8581 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 8641 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 8701 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 8761 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 8821 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 8881 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 8941 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 9001 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA 9061 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 9121 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 9181 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 9241 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 9301 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 9361 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA 9421 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACTCTAGGC AAAATAGCAC 9481 CCTCCCGCTC CAGAACAACA TACAGCGCTT CACAGCGGCA GCCTAACAGT CAGCCTTACC 9541 AGTAAAAAAG AAAACCTATT AAAAAAACAC CACTCGACAC GGCACCAGCT CAATCAGTCA 9601 CAGTGTAAAA AAGGGCCAAG TGCAGAGCGA GTATATATAG GACTAAAAAA TGACGTAACG 9661 GTTAAAGTCC ACAAAAAACA CCCAGAAAAC CGCACGCGAA CCTACGCCCA GAAACGAAAG 9721 CCAAAAAACC CACAACTTCC TCAAATCGTC ACTTCCGTTT TCCCACGTTA CGTAACTTCC 9781 CATTTTAAGA AAACTACAAT TCCCAACACA TACAAGTTAC TCCGCCCTAA AACCTACGTC 9841 ACCCGCCCCG TTCCCACGCC CCGCGCCACG TCACAAACTC CACCCCCTCA TTATCATATT 9901 GGCTTCAATC CAAAATAAGG TATA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.