pLV3-CMV-CDH17-3×FLAG-CopGFP-P

pLV3-CMV-CDH17-3×FLAG-CopGFP-Puro plasmid

PVT86684

pLV3-CMV-CDH17-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CDH17(human)-3×FLAG-CopGFP-Puro
Alias: NM_004063.4;CDH16; HPT-1; HPT1
Gene lenght: 2496bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CDH17-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CDH17-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of downstream genes, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-CDH17-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGA TACTTCAGGC CCATCTTCAC 2341 TCCCTGTGTC TTCTTATGCT TTATTTGGCA ACTGGATATG GCCAAGAGGG GAAGTTTAGT 2401 GGACCCCTGA AACCCATGAC ATTTTCTATT TATGAAGGCC AAGAACCGAG TCAAATTATA 2461 TTCCAGTTTA AGGCCAATCC TCCTGCTGTG ACTTTTGAAC TAACTGGGGA GACAGACAAC 2521 ATATTTGTGA TAGAACGGGA GGGACTTCTG TATTACAACA GAGCCTTGGA CAGGGAAACA 2581 AGATCTACTC ACAATCTCCA GGTTGCAGCC CTGGACGCTA ATGGAATTAT AGTGGAGGGT 2641 CCAGTCCCTA TCACCATAAA AGTGAAGGAC ATCAACGACA ATCGACCCAC GTTTCTCCAG 2701 TCAAAGTACG AAGGCTCAGT AAGGCAGAAC TCTCGCCCAG GAAAGCCCTT CTTGTATGTC 2761 AATGCCACAG ACCTGGATGA TCCGGCCACT CCCAATGGCC AGCTTTATTA CCAGATTGTC 2821 ATCCAGCTTC CCATGATCAA CAATGTCATG TACTTTCAGA TCAACAACAA AACGGGAGCC 2881 ATCTCTCTTA CCCGAGAGGG ATCTCAGGAA TTGAATCCTG CTAAGAATCC TTCCTATAAT 2941 CTGGTGATCT CAGTGAAGGA CATGGGAGGC CAGAGTGAGA ATTCCTTCAG TGATACCACA 3001 TCTGTGGATA TCATAGTGAC AGAGAATATT TGGAAAGCAC CAAAACCTGT GGAGATGGTG 3061 GAAAACTCAA CTGATCCTCA CCCCATCAAA ATCACTCAGG TGCGGTGGAA TGATCCCGGT 3121 GCACAATATT CCTTAGTTGA CAAAGAGAAG CTGCCAAGAT TCCCATTTTC AATTGACCAG 3181 GAAGGAGATA TTTACGTGAC TCAGCCCTTG GACCGAGAAG AAAAGGATGC ATATGTTTTT 3241 TATGCAGTTG CAAAGGATGA GTACGGAAAA CCACTTTCAT ATCCGCTGGA AATTCATGTA 3301 AAAGTTAAAG ATATTAATGA TAATCCACCT ACATGTCCGT CACCAGTAAC CGTATTTGAG 3361 GTCCAGGAGA ATGAACGACT GGGTAACAGT ATCGGGACCC TTACTGCACA TGACAGGGAT 3421 GAAGAAAATA CTGCCAACAG TTTTCTAAAC TACAGGATTG TGGAGCAAAC TCCCAAACTT 3481 CCCATGGATG GACTCTTCCT AATCCAAACC TATGCTGGAA TGTTACAGTT AGCTAAACAG 3541 TCCTTGAAGA AGCAAGATAC TCCTCAGTAC AACTTAACGA TAGAGGTGTC TGACAAAGAT 3601 TTCAAGACCC TTTGTTTTGT GCAAATCAAC GTTATTGATA TCAATGATCA GATCCCCATC 3661 TTTGAAAAAT CAGATTATGG AAACCTGACT CTTGCTGAAG ACACAAACAT TGGGTCCACC 3721 ATCTTAACCA TCCAGGCCAC TGATGCTGAT GAGCCATTTA CTGGGAGTTC TAAAATTCTG 3781 TATCATATCA TAAAGGGAGA CAGTGAGGGA CGCCTGGGGG TTGACACAGA TCCCCATACC 3841 AACACCGGAT ATGTCATAAT TAAAAAGCCT CTTGATTTTG AAACAGCAGC TGTTTCCAAC 3901 ATTGTGTTCA AAGCAGAAAA TCCTGAGCCT CTAGTGTTTG GTGTGAAGTA CAATGCAAGT 3961 TCTTTTGCCA AGTTCACGCT TATTGTGACA GATGTGAATG AAGCACCTCA ATTTTCCCAA 4021 CACGTATTCC AAGCGAAAGT CAGTGAGGAT GTAGCTATAG GCACTAAAGT GGGCAATGTG 4081 ACTGCCAAGG ATCCAGAAGG TCTGGACATA AGCTATTCAC TGAGGGGAGA CACAAGAGGT 4141 TGGCTTAAAA TTGACCACGT GACTGGTGAG ATCTTTAGTG TGGCTCCATT GGACAGAGAA 4201 GCCGGAAGTC CATATCGGGT ACAAGTGGTG GCCACAGAAG TAGGGGGGTC TTCCTTGAGC 4261 TCTGTGTCAG AGTTCCACCT GATCCTTATG GATGTGAATG ACAACCCTCC CAGGCTAGCC 4321 AAGGACTACA CGGGCTTGTT CTTCTGCCAT CCCCTCAGTG CACCTGGAAG TCTCATTTTC 4381 GAGGCTACTG ATGATGATCA GCACTTATTT CGGGGTCCCC ATTTTACATT TTCCCTCGGC 4441 AGTGGAAGCT TACAAAACGA CTGGGAAGTT TCCAAAATCA ATGGTACTCA TGCCCGACTG 4501 TCTACCAGGC ACACAGAGTT TGAGGAGAGG GAGTATGTCG TCTTGATCCG CATCAATGAT 4561 GGGGGTCGGC CACCCTTGGA AGGCATTGTT TCTTTACCAG TTACATTCTG CAGTTGTGTG 4621 GAAGGAAGTT GTTTCCGGCC AGCAGGTCAC CAGACTGGGA TACCCACTGT GGGCATGGCA 4681 GTTGGTATAC TGCTGACCAC CCTTCTGGTG ATTGGTATAA TTTTAGCAGT TGTGTTTATC 4741 CGCATAAAGA AGGATAAAGG CAAAGATAAT GTTGAAAGTG CTCAAGCATC TGAAGTCAAA 4801 CCTCTGAGAA GCGGATCCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 4861 GACTACAAGG ATGACGATGA CAAGTAGCGG CCGCGAAGGA TCTGCGATCG CTCCGGTGCC 4921 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 4981 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 5041 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 5101 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 5161 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 5221 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 5281 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 5341 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 5401 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGCTAGACGC CACCATGGAG 5461 AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC 5521 GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCAAGCAGGG CCGCATGACC 5581 AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG 5641 ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG 5701 CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC 5761 GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG 5821 GTGGTGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC 5881 AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG TGCTGGTGGG CAGCTTCGCC 5941 CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTTCG TGGTGGACAG CCACATGCAC 6001 TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC 6061 CGCGTGGAGG AGCTGCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC 6121 AAGACCCCCA TCGCCTTCGC CAGATCCCGC GCTCAGTCGT CCAATTCTGC CGTGGACGGC 6181 ACCGCCGGAC CCGGCTCCAC CGGATCTCGC GAGGGCAGAG GAAGTCTTCT AACATGCGGT 6241 GACGTGGAGG AGAATCCCGG CCCTATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC 6301 CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC 6361 ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC 6421 TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG 6481 GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC 6541 CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC 6601 CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC 6661 GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG 6721 CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG 6781 CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC 6841 ATGACCCGCA AGCCCGGTGC CTGAAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 6901 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 6961 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 7021 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 7081 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 7141 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 7201 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 7261 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 7321 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 7381 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 7441 GCCGCCTCCC CGCCTGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG 7501 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGATAAGA 7561 TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 7621 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 7681 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 7741 AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT 7801 TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA 7861 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 7921 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC 7981 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 8041 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 8101 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GACTTTTGCA GAGACCAAAT TCGTAATCAT 8161 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 8221 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 8281 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 8341 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 8401 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 8461 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 8521 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 8581 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 8641 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 8701 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 8761 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 8821 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 8881 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 8941 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 9001 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 9061 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 9121 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 9181 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 9241 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 9301 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 9361 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 9421 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 9481 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 9541 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 9601 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 9661 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 9721 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 9781 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 9841 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 9901 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 9961 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 10021 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 10081 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 10141 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 10201 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 10261 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA 10321 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG 10381 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 10441 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 10501 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 10561 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 10621 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 10681 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 10741 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.