pT3-EF1A-MYC-IRES-3×FLAG-Gpatc

pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4 plasmid

PVT86193

pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4 plasmid Information

Plasmid name: pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4(mouse)
Alias: NM_025663.4;2610029K21Rik; Gpatc4
Gene lenght: 1248bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pT3-EF1A-MYC-IRES-luc
Forward primer: IRES-F1
Reverse primer:
Copy number: High
Induction:

pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4 plasmid Description

pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4 This plasmid is suitable for overexpression in mammalian cells. The plasmid contains the EF1a strong promoter, which can initiate the transcription of the mouse Gpatch4 gene, promoting the N-terminal fusion expression of Myc and Flag tags for easy identification and tracking of the tagged target protein in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level, subcellular localization, and interactions with other proteins of the target protein. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pT3-EF1A-MYC-IRES-3×FLAG-Gpatch4 plasmid Sequence

1 CTAGCTCGAG ATCTTAATTA AACTAGTATC GATATAACTT CGTATAATGT ATGCTATACG 61 AAGTTATATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG GCTTTTGCAA 121 AAAGCTTTGC AAAGATGGAT AAAGTTTTAA ACAGAGAGGA ATCTTTGCAG CTAATGGACC 181 TTCTAGGTCT TGAAAGGAGT GGGAATTGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA 241 TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA 301 AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG 361 GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG 421 TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG 481 GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT GATTCTTGAT 541 CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA AGGAGCCCCT 601 TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT GCGAATCTGG 661 TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA AAATTTTTGA 721 TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG CCAAGATCTG 781 CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC GTCCCAGCGC 841 ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG GGGGTAGTCT 901 CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC CCCGCCCTGG 961 GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC 1021 CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA 1081 CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG 1141 TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA 1201 GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA 1261 GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA 1321 TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG 1381 TCGTGAGGAA TTAGCTTGGT ACTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGG 1441 TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATATCAA 1501 CAAGTTTGTA CAAAAAAGCA GGCTTCGCCA CCATGCCCCT CAACGTTAGC TTCACCAACA 1561 GGAACTATGA CCTCGACTAC GACTCGGTGC AGCCGTATTT CTACTGCGAC GAGGAGGAGA 1621 ACTTCTACCA GCAGCAGCAG CAGAGCGAGC TGCAGCCCCC GGCGCCCAGC GAGGATATCT 1681 GGAAGAAATT CGAGCTGCTG CCCACCCCGC CCCTGTCCCC TAGCCGCCGC TCCGGGCTCT 1741 GCTCGCCCTC CTACGTTGCG GTCACACCCT TCTCCCTTCG GGGAGACAAC GACGGCGGTG 1801 GCGGGAGCTT CTCCACGGCC GACCAGCTGG AGATGGTGAC CGAGCTGCTG GGAGGAGACA 1861 TGGTGAACCA GAGTTTCATC TGCGACCCGG ACGACGAGAC CTTCATCAAA AACATCATCA 1921 TCCAGGACTG TATGTGGAGC GGCTTCTCGG CCGCCGCCAA GCTCGTCTCA GAGAAGCTGG 1981 CCTCCTACCA GGCTGCGCGC AAAGACAGCG GCAGCCCGAA CCCCGCCCGC GGCCACAGCG 2041 TCTGCTCCAC CTCCAGCTTG TACCTGCAGG ATCTGAGCGC CGCCGCCTCA GAGTGCATCG 2101 ACCCCTCGGT GGTCTTCCCC TACCCTCTCA ACGACAGCAG CTCGCCCAAG TCCTGCGCCT 2161 CGCAAGACTC CAGCGCCTTC TCTCCGTCCT CGGATTCTCT GCTCTCCTCG ACGGAGTCCT 2221 CCCCGCAGGG CAGCCCCGAG CCCCTGGTGC TCCATGAGGA GACACCGCCC ACCACCAGCA 2281 GCGACTCTGA GGAGGAACAA GAAGATGAGG AAGAAATCGA TGTTGTTTCT GTGGAAAAGA 2341 GGCAGGCTCC TGGCAAAAGG TCAGAGTCTG GATCACCTTC TGCTGGAGGC CACAGCAAAC 2401 CTCCTCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACACATCAG CACAACTACG 2461 CAGCGCCTCC CTCCACTCGG AAGGACTATC CTGCTGCCAA GAGGGTCAAG TTGGACAGTG 2521 TCAGAGTCCT GAGACAGATC AGCAACAACC GAAAATGCAC CAGCCCCAGG TCCTCGGACA 2581 CCGAGGAGAA TGTCAAGAGG CGAACACACA ACGTCTTGGA GCGCCAGAGG AGGAACGAGC 2641 TAAAACGGAG CTTTTTTGCC CTGCGTGACC AGATCCCGGA GTTGGAAAAC AATGAAAAGG 2701 CCCCCAAGGT AGTTATCCTT AAAAAAGCCA CAGCATACAT CCTGTCCGTC CAAGCAGAGG 2761 AGCAAAAGCT CATTTCTGAA GAGGACTTGT TGCGGAAACG ACGAGAACAG TTGAAACACA 2821 AACTTGAACA GCTACGGAAC TCTTGTGCGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 2881 TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGCG GTTCGAAGGT AAGCCTATCC 2941 CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTTA GTAATGAGTT TCCCCCCCCC 3001 CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA 3061 TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC 3121 TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT 3181 GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC 3241 CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT 3301 GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT 3361 GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG 3421 AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT 3481 TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA 3541 AAAACACGAT GATAATATGG CCACAACCGC CACCATGGAC TACAAAGACC ATGACGGTGA 3601 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGATGAGTG TCACCCCAGA 3661 GGTCAAGAGC CGTGGGATGA AGTTTGCTGA AGAGCAGCTG CTGAAGCATG GATGGACTCA 3721 AGGCAAGGGC CTGGGCCGGA GGGAGAATGG CATTACCCAG GCCCTCAAGG TGACACTGAA 3781 GCAAGATAAC CATGGGGTGG GACATGACCC TGCCAAGGAA TTTACAGACC ACTGGTGGAG 3841 TGATCTCTTC AACAAGACTG CCGCCAGCTT GGTAGTGGAC TCGGGGAAGG ATGGAGTGCA 3901 GATAAGACGT CTTTCCAAGG AGACCACCCA GCGCAGTCAC CCCAAACCCA GCTTGCTGTA 3961 TCAGAAGTTT GTGAAGACAG CCACACTAAC CTCGGGTGAG GAGAAGCCTG ACAGAGACTT 4021 GGGGAACTGC AGTGATGTTG ACAACCATGA GCCCACGCCC CCAAAGATTC TGACTGATGA 4081 GATGCTACTC AAGGCTTGCG AGGGGCGAAC AGCACACAAG GCTGCCCGGA TTGGTATCAC 4141 CATGAAGGCC AAGCTTGCTC GCCTGGAGGC CCAAGAGCAG GCCTTTCTGG CTCAGCTCAA 4201 AGGCTCAAAG GCTCTGGGCA CCTCTCAACC ACTGACTGAC AGTGAGCCTT CCCAAAAAAA 4261 GAAAAAGAAG AGGAAGCAGA AAGAGGGGGA AGAGGCTGCA ACAACTGAAA AGAGTCTAGG 4321 TGACGAGCTG CTGGGACACA CGGACAGGAG CTTCAGGGAC AGCAGGAAGA AGAAGAAGAG 4381 GCAGAAAGCC GAGAGACAGG GAACGGCTAT AGGAAGTGAG GAGGAAGAGG CTGCAGGAGA 4441 GAGTGGGCCC AGAGAACTGA GCACAGAGCA ATCTGATCAG CCTTCCAGGA AAAAGAAGAA 4501 AAGGAGGAAG CAGCGCCATG AAGAAGACGG GGAGATGGGA GTCTGTGATG AAGGAGGTAG 4561 AGATGTGACA AGCCGGCCAA AGGCAGTCAA CAGTGGAGGA GACAAAGACC CCAGGAGAAG 4621 CAGCAAGAAG CGAGGGACGT GTGGGGAGGA CTTAGATACC CAAGAGGAAG AAGGGAAGGA 4681 TGACCTAACA AAAGGAGAGA GAAAGGTCAG AAGAAAAGAC AAAAGGAAAA GACAGCAGTG 4741 CTGTGAGGAG GACTTAGATG TAAGCAGTAA AGATGATGGT GGGACATGGG TAGCAGAGGA 4801 TGCAGGTGAG AGAAGTCGGC AATACCCAAA GGAGAGAGCC AAAAAGAAGA AGCGGAAGAG 4861 GGACCGAGGG TCTGAAGTGG ATCTCAGCTG ATTCTAGGAT CCGTCGACCT GCAGCCAAGC 4921 TTATCGATAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 4981 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 5041 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 5101 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 5161 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA 5221 CGCGCCCTGT AGCGGCGCAT TAAATAACTT CGTATAATGT ATGCTATACG AAGTTATATC 5281 GTATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC 5341 TGACACACAT TCCACAGAAT TCCTTGAAAT ACATCCACAG GTACACCTCC AATTGACTCA 5401 AATGATGTCA ATTAGTCTAT CAGAAGCTTC TAAAGCCATG ACATCATTTT CTGGAATTTT 5461 CCAAGCTGTT TAAAGGCACA GTCAACTTAG TGTATGTAAA CTTCTGACCC ACTGGAATTG 5521 TGATACAGTG AATTATAAGT GAAATAATCT GTCTGTAAAC AATTGTTGGA AAAATGACTT 5581 GTGTCATGCA CAAAGTAGAT GTCCTAACTG ACTTGCCAAA ACTATTGTTT GTTAACAAGA 5641 AATTTGTGGA GTAGTTGAAA AACGAGTTTT AATGACTCCA ACTTAAGTGT ATGTAAACTT 5701 CCGACTTCAA CTGTAGGGTA CCGAGCTCGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG 5761 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 5821 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA 5881 ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC 5941 GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC 6001 ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA 6061 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA 6121 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 6181 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 6241 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 6301 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 6361 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 6421 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 6481 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA 6541 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 6601 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 6661 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 6721 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 6781 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 6841 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 6901 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 6961 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 7021 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 7081 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 7141 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 7201 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 7261 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 7321 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 7381 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 7441 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 7501 ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 7561 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 7621 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 7681 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 7741 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 7801 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 7861 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 7921 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 7981 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 8041 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 8101 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 8161 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 8221 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 8281 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA 8341 ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA GGTCGACTCT AGACCTATAC 8401 AGTTGAAGTC GGAAGTTTAC ATACACTTAA GTTGGAGTCA TTAAAACTCG TTTTTCAACT 8461 ACTCCACAAA TTTCTTGTTA ACAAACAATA GTTTTGGCAA GTCAGTTAGG ACATCTACTT 8521 TGTGCATGAC ACAAGTCATT TTTCCAACAA TTGTTTACAG ACAGATTATT TCACTTATAA 8581 TTCACTGTAT CACAATTCCA GTGGGTCAGA AGTTTACATA CACTAAGTTG ACTGTGCCTT 8641 TAAACAGCTT GGAAAATTCC AGAAAATGAT GTCATGGCTT TAGAAGCTTC TGATAGACTA 8701 ATTGACATCA TTTGAGTCAA TTGGAGGTGT ACCTGTGGAT GTATTTCAAG GGGATCCAGA 8761 CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGC GGCCG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.