pT3-EF1A-MYC-IRES-3×FLAG-Stk35

pT3-EF1A-MYC-IRES-3×FLAG-Stk35 plasmid

PVT86189

pT3-EF1A-MYC-IRES-3×FLAG-Stk35 plasmid Information

Plasmid name: pT3-EF1A-MYC-IRES-3×FLAG-Stk35(mouse)
Alias: NM_183262.3;1700054C12Rik; CLIK1; Stk35l1
Gene lenght: 1620bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pT3-EF1A-MYC-IRES-luc
Forward primer: IRES-F1
Reverse primer:
Copy number: High
Induction:

pT3-EF1A-MYC-IRES-3×FLAG-Stk35 plasmid Description

pT3-EF1A-MYC-IRES-3×FLAG-Stk35 This plasmid is suitable for overexpression in mammalian cells. The plasmid contains the EF1a strong promoter, which can initiate the transcription of the mouse Stk35 gene, promoting the N-terminal fusion expression of Myc and Flag tags for easy identification and tracking of the tagged target protein in experiments such as Western blotting, immunoprecipitation, or immunofluorescence, thereby studying the expression level, subcellular localization, and interactions with other proteins of the target protein. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pT3-EF1A-MYC-IRES-3×FLAG-Stk35 plasmid Sequence

1 CTAGCTCGAG ATCTTAATTA AACTAGTATC GATATAACTT CGTATAATGT ATGCTATACG 61 AAGTTATATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG GCTTTTGCAA 121 AAAGCTTTGC AAAGATGGAT AAAGTTTTAA ACAGAGAGGA ATCTTTGCAG CTAATGGACC 181 TTCTAGGTCT TGAAAGGAGT GGGAATTGGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA 241 TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA 301 AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG 361 GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG 421 TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG 481 GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT GATTCTTGAT 541 CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA AGGAGCCCCT 601 TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT GCGAATCTGG 661 TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA AAATTTTTGA 721 TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG CCAAGATCTG 781 CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC GTCCCAGCGC 841 ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG GGGGTAGTCT 901 CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC CCCGCCCTGG 961 GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC 1021 CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA 1081 CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG 1141 TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA 1201 GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA 1261 GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA 1321 TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG 1381 TCGTGAGGAA TTAGCTTGGT ACTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGG 1441 TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATATCAA 1501 CAAGTTTGTA CAAAAAAGCA GGCTTCGCCA CCATGCCCCT CAACGTTAGC TTCACCAACA 1561 GGAACTATGA CCTCGACTAC GACTCGGTGC AGCCGTATTT CTACTGCGAC GAGGAGGAGA 1621 ACTTCTACCA GCAGCAGCAG CAGAGCGAGC TGCAGCCCCC GGCGCCCAGC GAGGATATCT 1681 GGAAGAAATT CGAGCTGCTG CCCACCCCGC CCCTGTCCCC TAGCCGCCGC TCCGGGCTCT 1741 GCTCGCCCTC CTACGTTGCG GTCACACCCT TCTCCCTTCG GGGAGACAAC GACGGCGGTG 1801 GCGGGAGCTT CTCCACGGCC GACCAGCTGG AGATGGTGAC CGAGCTGCTG GGAGGAGACA 1861 TGGTGAACCA GAGTTTCATC TGCGACCCGG ACGACGAGAC CTTCATCAAA AACATCATCA 1921 TCCAGGACTG TATGTGGAGC GGCTTCTCGG CCGCCGCCAA GCTCGTCTCA GAGAAGCTGG 1981 CCTCCTACCA GGCTGCGCGC AAAGACAGCG GCAGCCCGAA CCCCGCCCGC GGCCACAGCG 2041 TCTGCTCCAC CTCCAGCTTG TACCTGCAGG ATCTGAGCGC CGCCGCCTCA GAGTGCATCG 2101 ACCCCTCGGT GGTCTTCCCC TACCCTCTCA ACGACAGCAG CTCGCCCAAG TCCTGCGCCT 2161 CGCAAGACTC CAGCGCCTTC TCTCCGTCCT CGGATTCTCT GCTCTCCTCG ACGGAGTCCT 2221 CCCCGCAGGG CAGCCCCGAG CCCCTGGTGC TCCATGAGGA GACACCGCCC ACCACCAGCA 2281 GCGACTCTGA GGAGGAACAA GAAGATGAGG AAGAAATCGA TGTTGTTTCT GTGGAAAAGA 2341 GGCAGGCTCC TGGCAAAAGG TCAGAGTCTG GATCACCTTC TGCTGGAGGC CACAGCAAAC 2401 CTCCTCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACACATCAG CACAACTACG 2461 CAGCGCCTCC CTCCACTCGG AAGGACTATC CTGCTGCCAA GAGGGTCAAG TTGGACAGTG 2521 TCAGAGTCCT GAGACAGATC AGCAACAACC GAAAATGCAC CAGCCCCAGG TCCTCGGACA 2581 CCGAGGAGAA TGTCAAGAGG CGAACACACA ACGTCTTGGA GCGCCAGAGG AGGAACGAGC 2641 TAAAACGGAG CTTTTTTGCC CTGCGTGACC AGATCCCGGA GTTGGAAAAC AATGAAAAGG 2701 CCCCCAAGGT AGTTATCCTT AAAAAAGCCA CAGCATACAT CCTGTCCGTC CAAGCAGAGG 2761 AGCAAAAGCT CATTTCTGAA GAGGACTTGT TGCGGAAACG ACGAGAACAG TTGAAACACA 2821 AACTTGAACA GCTACGGAAC TCTTGTGCGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 2881 TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGCG GTTCGAAGGT AAGCCTATCC 2941 CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTTA GTAATGAGTT TCCCCCCCCC 3001 CCTAACGTTA CTGGCCGAAG CCGCTTGGAA TAAGGCCGGT GTGCGTTTGT CTATATGTTA 3061 TTTTCCACCA TATTGCCGTC TTTTGGCAAT GTGAGGGCCC GGAAACCTGG CCCTGTCTTC 3121 TTGACGAGCA TTCCTAGGGG TCTTTCCCCT CTCGCCAAAG GAATGCAAGG TCTGTTGAAT 3181 GTCGTGAAGG AAGCAGTTCC TCTGGAAGCT TCTTGAAGAC AAACAACGTC TGTAGCGACC 3241 CTTTGCAGGC AGCGGAACCC CCCACCTGGC GACAGGTGCC TCTGCGGCCA AAAGCCACGT 3301 GTATAAGATA CACCTGCAAA GGCGGCACAA CCCCAGTGCC ACGTTGTGAG TTGGATAGTT 3361 GTGGAAAGAG TCAAATGGCT CTCCTCAAGC GTATTCAACA AGGGGCTGAA GGATGCCCAG 3421 AAGGTACCCC ATTGTATGGG ATCTGATCTG GGGCCTCGGT GCACATGCTT TACATGTGTT 3481 TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT TTTCCTTTGA 3541 AAAACACGAT GATAATATGG CCACAACCGC CACCATGGAC TACAAAGACC ATGACGGTGA 3601 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGATGGGCC ACCAGGAATC 3661 TCCGCTGACC AGAGCGGCGG CGGGAGGTGC AGCTTACATA AAGAGGTTAC GTAAAGTGCT 3721 CAGCTGGCGT GAACTCGGGG ACGGCCACGG GAACCTGGAA GCCGAGGCCT CCCCCGGGAG 3781 CGTTGCTGTC ATCACAAGAG CAGCGCCGCG ACGCGCTACA CGCTCAGCCA GGCTCCCCGC 3841 CTCGCGCCCT ACCCGGCTCT GCAGACAAGC CCGGCTCGGA ACGGACCATC CTCCGGCGCG 3901 GGCTCCAAGG GGGAACCGGT TCGCCCGGAA GAGGAACAGC GCCGGCCAGA TCACCATCCA 3961 AGGTCCCGCT CCTCCACATC TCGGGGCTAG ACGGAGGGAT GAGGCAAGGG GGGCCCGGGC 4021 GGCGCCGTTG CTGCTCCCCC CGCCCCCCGC AGCCATGGAA ACGGGGAAAG AGAACGGAGC 4081 CCGCAGAGGG ACAAAAAGCC CGGAGCGGAA AAGGCGAAGC CCAGTCCAGC GGGTACTGTG 4141 CGAGAAGCTG AGGCCGGCGG CCCAGGCCAT GGATCCGGCT GGGGCCGAGG TCCCGGGCGA 4201 GGCCTTCCTG GCCCGGCGGC GGCCGGATGG CGGCGGCGGG GATGTTCCTG CACGGCCGCG 4261 CTACAGCCTC TTGGCGGAGA TCGGGCGCGG CAGCTACGGC GTGGTTTATG AGGCTGTGGC 4321 TGGGCGCAGT GGGGCCAGGG TGGCAGTCAA GAAGATCCGC TGCGACGCTC CCGAGAACGT 4381 GGAGTTGGCA CTAGCAGAAT TCTGGGCCCT CACCAGTCTC AAGCGGCGGC ACCAGAATAT 4441 CGTGCAGTTT GAGGAGTGCG TCCTACAGCG CAACGGGTTA GCCCAGCGCA TGAGTCACGG 4501 CAACAAGAAC TCACAGCTTT ACCTGCGCCT GGTGGAGACC TCGCTCAAAG GAGAAAGGAT 4561 CCTGGGCTAT GCTGAGGAGC CCTGCTATCT CTGGTTTGTC ATGGAGTACT GTGAAGGTGG 4621 AGACCTCAAT CAGTATGTCC TGTCCCGGAG ACCTGACCCA GCCACCAACA AAAGTTTCAT 4681 GCTACAGCTT ACAAGCGCCA TTGCCTTCCT GCATAAAAAC CACATCGTGC ACAGGGACCT 4741 AAAGCCAGAC AACATCCTGA TCACAGAGCG GTCTGGCACC CCCATCCTCA AGGTGGCAGA 4801 CTTTGGACTG AGCAAGGTCT GTGCAGGGCT GGCACCCCGA GGCAAAGAGG GCAATCAAGA 4861 TAACAAAAAT GTGAATGTGA ATAAATACTG GCTGTCCTCA GCTTGTGGCT CAGACTTCTA 4921 CATGGCTCCC GAAGTCTGGG AGGGACACTA TACAGCCAAG GCGGACATCT TTGCTCTGGG 4981 CATTATCATC TGGGCAATGA TAGAAAGAAT TACCTTTATT GACTCTGAAA CCAAGAAGGA 5041 GCTCCTGGGG ACCTACATTA AGCAAGGGAC TGAGATCGTC CCTGTTGGTG AGGCGCTGCT 5101 AGAAAACCCA AAGATGGAGT TGCATATCCC CCAGAAACGT AGGACTTCCA TGTCTGAGGG 5161 GGTCAAGCAG CTCTTGAAAG ACATGTTAGC TGCTAACCCA CAGGACCGAC CTGATGCTTT 5221 TGAACTTGAA ACCCGAATGG ACCAGGTCAC ATGTGCTGCT TAATTCTAGG ATCCGTCGAC 5281 CTGCAGCCAA GCTTATCGAT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA 5341 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 5401 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 5461 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 5521 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG 5581 GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAATAAC TTCGTATAAT GTATGCTATA 5641 CGAAGTTATA TCGTATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT 5701 CCACACCCTA ACTGACACAC ATTCCACAGA ATTCCTTGAA ATACATCCAC AGGTACACCT 5761 CCAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT TCTAAAGCCA TGACATCATT 5821 TTCTGGAATT TTCCAAGCTG TTTAAAGGCA CAGTCAACTT AGTGTATGTA AACTTCTGAC 5881 CCACTGGAAT TGTGATACAG TGAATTATAA GTGAAATAAT CTGTCTGTAA ACAATTGTTG 5941 GAAAAATGAC TTGTGTCATG CACAAAGTAG ATGTCCTAAC TGACTTGCCA AAACTATTGT 6001 TTGTTAACAA GAAATTTGTG GAGTAGTTGA AAAACGAGTT TTAATGACTC CAACTTAAGT 6061 GTATGTAAAC TTCCGACTTC AACTGTAGGG TACCGAGCTC GAATTCACTG GCCGTCGTTT 6121 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 6181 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT 6241 TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG 6301 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA 6361 GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG 6421 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC 6481 CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 6541 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 6601 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 6661 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 6721 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 6781 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 6841 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 6901 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 6961 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 7021 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 7081 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 7141 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 7201 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 7261 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 7321 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 7381 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 7441 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 7501 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 7561 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 7621 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 7681 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 7741 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 7801 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 7861 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 7921 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 7981 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 8041 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 8101 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 8161 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 8221 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 8281 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 8341 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 8401 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 8461 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 8521 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 8581 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 8641 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 8701 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT 8761 CTAGACCTAT ACAGTTGAAG TCGGAAGTTT ACATACACTT AAGTTGGAGT CATTAAAACT 8821 CGTTTTTCAA CTACTCCACA AATTTCTTGT TAACAAACAA TAGTTTTGGC AAGTCAGTTA 8881 GGACATCTAC TTTGTGCATG ACACAAGTCA TTTTTCCAAC AATTGTTTAC AGACAGATTA 8941 TTTCACTTAT AATTCACTGT ATCACAATTC CAGTGGGTCA GAAGTTTACA TACACTAAGT 9001 TGACTGTGCC TTTAAACAGC TTGGAAAATT CCAGAAAATG ATGTCATGGC TTTAGAAGCT 9061 TCTGATAGAC TAATTGACAT CATTTGAGTC AATTGGAGGT GTACCTGTGG ATGTATTTCA 9121 AGGGGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA 9181 GCGGCCG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.