小鼠程序性死亡分子1 ELISA定量试剂盒(PD-1)

小鼠程序性死亡分子 1 (PD-1) ELISA 定量试剂盒

英文名称：Mouse PD-1 ELISA Kit  |  中文名称：小鼠程序性死亡分子1 ELISA定量试剂盒（PD-1）
货号：KTE3109-1  |  应用领域：蛋白类 → ELISA → 细胞分析（核心产品）

关键产品信息

• 检测类型：ELISA（夹心法）
• 适用物种：小鼠（Mouse）
• 样本类型：细胞培养上清、血清、血浆
• 检测指标：PD-1 蛋白浓度
• 检测范围：78.13 pg/mL – 5,000 pg/mL
• 检出限（灵敏度）：78.13 pg/mL

核心优势

• 极高的灵敏度和特异性，适用于低丰度 PD-1 检测。
• 经验证的夹心法设计，交叉反应及背景干扰极低。
• 配套完整的 48 T 与 96 T 套装，满足不同实验规模需求。
• 采用专有链霉亲和素‑HRP 复合物，显色反应快速、可重复性好。

检测原理概述

本套件基于双抗体夹心 ELISA 工作原理：

1. 微孔板预包被特异性抗小鼠 PD-1 抗体（固相捕获抗体）。
2. 加入待测样品或标准品，PD-1 蛋白与捕获抗体形成第一层免疫复合物。
3. 洗脱未结合成分后，加入生物素化的检测抗体，使其与已捕获的 PD-1 再次结合。
4. 加入链霉亲和素‑HRP，利用生物素‑链霉亲和素的高亲和性形成信号放大复合物。
5. 加入 TMB 底物，HRP 催化显色（蓝色），随后加入终止液转为黄色。
6. 在 450 nm 波长下读取 OD 值，OD 与 PD-1 浓度呈正比。

试剂盒组成

• Mouse PD-1 Microplate
• Mouse PD-1 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse PD-1 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用要点与注意事项

• 不同批号或过期的试剂严禁混用。
• 使用前请在室温平衡 30 分钟；所有操作建议使用一次性吸头以防交叉污染。
• 未使用的板条请及时放回包装，密封后置于 4 ℃保存。
• 充分混匀对结果影响显著，推荐使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品和标准品建议做双孔或三孔（复孔）检测，以提高数据可靠性。

储存与运输

• 未开封的试剂盒请避光存于 2‑8 ℃，有效期 24 个月。
• 启用后请参照说明书分别储存各组分（部分组分需冷冻或在 4 ℃保存）。
• 运输方式：冰袋（蓝冰）冷链运输，确保全程温控。

技术规格与采购信息

规格	数量（孔）	单价（人民币）	交货期
48 T	48	1,598.00	请咨询
96 T	96	2,558.00	期货（5 天内发货）

重要声明

本产品仅供科研使用，不适用于人类临床诊断或治疗。使用本套件进行的任何专利或许可相关行为，风险与责任由使用者自行承担。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Programmed Cell Death Protein 1 (PD‑1) ELISA Quantification Kit

English Name: Mouse PD‑1 ELISA Kit  |  Chinese Name: Mouse Programmed Cell Death Protein 1 ELISA Quantification Kit (PD‑1)
Catalog No.: KTE3109-1  |  Application Area: Protein → ELISA → Cell Analysis (Core Product)

Key Product Information

• Assay Type: Sandwich ELISA
• Applicable Species: Mouse
• Sample Types: Cell culture supernatant, serum, plasma
• Analyte: PD‑1 protein concentration
• Dynamic Range: 78.13 pg/mL – 5,000 pg/mL
• Limit of Detection (Sensitivity): 78.13 pg/mL

Core Advantages

• Exceptionally high sensitivity and specificity, suitable for detecting low‑abundance PD‑1.
• Validated sandwich design with minimal cross‑reactivity and background interference.
• Complete 48‑well and 96‑well formats available to accommodate varying experimental scales.
• Incorporates proprietary streptavidin‑HRP complex for rapid color development and excellent reproducibility.

Assay Principle Overview

This kit is based on a dual‑antibody sandwich ELISA principle:

1. Microplate is pre‑coated with a specific anti‑mouse PD‑1 capture antibody (solid‑phase capture).
2. Test samples or standards are added; PD‑1 binds the capture antibody to form the first immune complex.
3. After washing away unbound material, a biotinylated detection antibody is added to bind the captured PD‑1.
4. Streptavidin‑HRP is added, forming a signal‑amplifying complex via the high‑affinity biotin–streptavidin interaction.
5. TMB substrate is added; HRP catalyzes color development (blue), followed by stop solution turning the reaction yellow.
6. Optical density is measured at 450 nm; OD is proportional to PD‑1 concentration.

Kit Components

• Mouse PD‑1 Microplate
• Mouse PD‑1 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse PD‑1 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Critical Use Points and Precautions

• Do not mix reagents from different lot numbers or expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use; employ disposable pipette tips to avoid cross‑contamination.
• Return unused strips to their packaging promptly, seal, and store at 4 °C.
• Thorough mixing critically influences results; recommend gentle vortexing or manual inversion every 10 minutes.
• Perform samples and standards in duplicate or triplicate wells to enhance data reliability.

Storage and Shipping

• Store unopened kits protected from light at 2‑8 °C; shelf life is 24 months.
• After opening, store individual components per the manual (some require freezing, others at 4 °C).
• Shipping: cold chain using insulated ice packs (blue ice) to maintain temperature control throughout transit.

Technical Specifications and Purchasing Information

Format	Number of Wells	Unit Price (CNY)	Lead Time
48 well	48	1,598.00	Please inquire
96 well	96	2,558.00	Stock (ship within 5 days)

Important Statement

This product is for research use only and is not intended for human clinical diagnosis or therapy. Any patent or licensing activities associated with the use of this kit are the sole responsibility and risk of the user.

Note: The above information may change due to product optimization or upgrades; for the latest details, please contact Akorn Company.