EliKine™ 小鼠程序性死亡分子1 ELISA定量试剂盒

EliKine™ 小鼠程序性死亡分子1 ELISA定量试剂盒

货号：KTE3109 | 分子靶标：PD-1 (CD279)

核心特性

• ⧫ 超敏检测：最低检测限78.13 pg/mL，动态范围5,000-78.13 pg/mL
• ⧫ 三重样本适配：细胞培养上清/血清/血浆同步兼容
• ⧫ 专利组分体系：预包被板+冻存标准品+超稳20×浓缩洗液
• ⧫ 双抗验证：经生物素化检测抗体与链霉亲和素-HRP双级放大系统验证

技术规格

检测原理	夹心法ELISA（双抗体表位结合）
板载规格	48T（1598元） | 96T（2558元）
质控保障	批内CV＜8% | 批间CV＜10%
冷链运输	蓝冰全程温控（2-8℃活性保障）

关键应用场景

▶ 自身免疫疾病研究模型构建
▶ T细胞耗竭状态定量分析
▶ PD-1/PD-L1通路抑制剂药效评估

操作里程碑

1. 预包被板常温平衡30分钟
2. 标准品梯度复溶（推荐三复孔）
3. HRP-TMB显色系统10分钟终止
4. 酶标仪450nm双波长校正

*本产品符合ISO13485质量体系，仅供科研使用*

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Programmed Death-1 ELISA Quantitation Kit

Cat#: KTE3109 | Target: PD-1 (CD279)

Core Features

• ⧫ Ultra-Sensitive Detection: LOD 78.13 pg/mL, dynamic range 5,000-78.13 pg/mL
• ⧫ Triple Sample Compatibility: Validated for cell culture supernatant/serum/plasma
• ⧫ Proprietary Formulation: Pre-coated plates + lyophilized standards + stabilized 20× wash buffer
• ⧫ Dual-Antibody Verification: Validated with biotinylated detection antibody and streptavidin-HRP amplification system

Technical Specifications

Principle	Sandwich ELISA (Dual Antibody Epitope Binding)
Plate Format	48T (¥1,598) | 96T (¥2,558)
QC Parameters	Intra-assay CV <8% | Inter-assay CV <10%
Shipping	Cold-chain transport with blue ice (2-8℃ activity preservation)

Key Applications

▶ Construction of autoimmune disease research models
▶ Quantitative analysis of T-cell exhaustion status
▶ Efficacy evaluation of PD-1/PD-L1 pathway inhibitors

Operational Protocol

1. Equilibrate pre-coated plate at ambient temperature for 30 min
2. Reconstitute standard gradients (triplicate recommended)
3. Terminate HRP-TMB chromogenic reaction at 10 min
4. Perform dual-wavelength calibration at 450 nm using microplate reader

*Manufactured under ISO13485 quality system. For research use only*

Note: Specifications are subject to change due to product optimization. Contact Abbkine for latest information.