PDGF-BB ELISA KIT 血小板衍生生长因子BBE

Mouse PDGF-BB ELISA Kit - 小鼠血小板衍生生长因子BB ELISA试剂盒 (KTE3095-2)

产品概述

本试剂盒为EliKine™系列核心产品，专门设计用于高灵敏度、高特异性定量检测小鼠样本（包括细胞培养上清、血清、血浆）中的血小板衍生生长因子BB (PDGF-BB) 蛋白含量。采用经典的夹心法ELISA原理，确保检测结果的准确性与可靠性，是细胞功能分析与信号通路研究的理想工具。

关键特性与优势

• 高灵敏度：最低检测浓度可达 23.44 pg/mL，能够精准捕捉低丰度表达。
• 宽检测范围：23.44 pg/mL - 1,500 pg/mL，涵盖广泛的生理及实验浓度。
• 优异特异性：经验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 即用型组分：提供预包被微孔板、冻干标准品及全套优化缓冲液，简化操作流程。
• 出色的批次稳定性：严格的质量控制确保不同批次间结果的一致性。

产品规格与价格

规格	货号	价格 (人民币)	货期
48 T (测试)	KTE3095-2	1,598.0	请咨询
96 T (测试)	KTE3095-2	2,558.0	请咨询

试剂盒组分

• 小鼠PDGF-BB预包被微孔板 (Mouse PDGF-BB Microplate)
• 小鼠PDGF-BB标准品 (冻干) (Mouse PDGF-BB Standard, lyophilized)
• 标准品稀释液 (Standard Diluent)
• 检测缓冲液 (10×) (Assay Buffer 10×)
• 小鼠PDGF-BB检测抗体 (100×) (Mouse PDGF-BB Detect Antibody 100×)
• 链霉亲和素-HRP (100×) (Streptavidin-HRP 100×)
• HRP底物 (TMB) (HRP Substrate TMB)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×) (Wash Buffer 20×)
• 板盖 (Plate Covers)

背景信息

血小板衍生生长因子(PDGF)是由A、B两条多肽链通过二硫键连接形成的二聚体糖蛋白，其中PDGF-BB在血管生成、组织修复、炎症反应及肿瘤发生发展中扮演关键角色。它主要由血小板、巨噬细胞、内皮细胞等分泌，通过促进相关细胞增殖、迁移，参与多种生理病理过程。本试剂盒精准靶向小鼠PDGF-BB，为相关领域的基础研究与药物开发提供有力的定量分析手段。

保存与运输

保存建议：未开封试剂盒请避光保存于2-8℃，有效期24个月。启用后，请参照说明书分别保存各组分。

运输条件：全程蓝冰（冰袋）运输，以确保试剂活性。

重要注意事项

• 请勿混用不同批号或过期试剂。
• 使用前，所有试剂需室温平衡30分钟。
• 为避免交叉污染，请使用一次性吸头。
• 未使用的板条应立即放回铝箔袋并密封，4℃保存。
• 实验过程中，充分混匀对结果至关重要，建议使用振荡器或定期手动摇匀。
• 为保证结果准确性，建议对样品和标准品进行复孔检测（双孔或三孔）。

警告：本产品仅限用于科学研究，不得用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse PDGF-BB ELISA Kit (KTE3095-2)

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for the high-sensitivity and high-specificity quantitative detection of Platelet-Derived Growth Factor-BB (PDGF-BB) protein levels in mouse samples (including cell culture supernatants, serum, and plasma). Utilizing the classic sandwich ELISA principle, it ensures accurate and reliable results, making it an ideal tool for cell function analysis and signaling pathway research.

Key Features & Advantages

• High Sensitivity: Minimum detectable concentration as low as 23.44 pg/mL, enabling precise detection of low-abundance expression.
• Wide Detection Range: 23.44 pg/mL - 1,500 pg/mL, covering a broad spectrum of physiological and experimental concentrations.
• Excellent Specificity: Validated to show no significant cross-reactivity or background interference with analogous proteins.
• Ready-to-Use Components: Provides pre-coated microplates, lyophilized standards, and a complete set of optimized buffers to streamline the operational procedure.
• Outstanding Lot-to-Lot Consistency: Rigorous quality control ensures consistent results across different lots.

Specifications & Price

Specification	Catalog Number	Price (RMB)	Availability
48 T (Tests)	KTE3095-2	1,598.0	Please Inquire
96 T (Tests)	KTE3095-2	2,558.0	Please Inquire

Kit Components

• Mouse PDGF-BB Pre-coated Microplate
• Mouse PDGF-BB Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse PDGF-BB Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Background Information

Platelet-Derived Growth Factor (PDGF) is a dimeric glycoprotein composed of A and B polypeptide chains linked by disulfide bonds. PDGF-BB plays a critical role in angiogenesis, tissue repair, inflammatory responses, and tumorigenesis. It is primarily secreted by platelets, macrophages, endothelial cells, etc., and participates in various physiological and pathological processes by promoting the proliferation and migration of relevant cells. This kit specifically targets mouse PDGF-BB, providing a powerful quantitative analysis tool for basic research and drug development in related fields.

Storage & Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8°C and are valid for 24 months. After opening, store individual components according to the instructions.

Shipping Condition: Shipped with blue ice (ice packs) throughout to ensure reagent activity.

Important Notes

• Do not mix reagents from different lots or use expired reagents.
• Before use, all reagents must be equilibrated to room temperature for 30 minutes.
• Use disposable pipette tips to avoid cross-contamination.
• Immediately return unused strips to the foil pouch and seal them for storage at 4°C.
• Thorough mixing is crucial during the assay; the use of a plate shaker or periodic manual shaking is recommended.
• For accurate results, it is recommended to perform duplicate or triplicate measurements for both samples and standards.

Warning: This product is for research use only. Not for use in humans or clinical diagnosis.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.