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产品简介:
TG1 电转感受态细胞是一种经过特殊制备、具有超高电穿孔转化效率的大肠杆菌(Escherichia coli)K-12 衍生菌株。本产品专为需要高效导入大质粒、复杂文库(如噬菌体展示文库、cDNA 文库)或BACs (细菌人工染色体) 等应用场景而优化。lacIqZΔM15 的存在使其可用于蓝白斑筛选等实验;野生型核酸酶 endA1,体内核酸酶含量较高,提取质粒时推荐使用质粒提取试剂盒中去蛋白液以去除菌体内大量的核酸酶。本产品采用优化的电转感受态制备工艺,使用 pUC19 标准质粒检测,转化效率高达 1.0×10^10 cfu/µg DNA。
产品应用:
♦本产品主要适用于大质粒转化、噬菌体展示文库以及克隆构建。
产品特点:
♦ 转化效率高,高达 1.0×10^10cfu/μg。
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文献和实验Method for Single-Cell Electroporation
Inroduction Single-cell electroporation (SCE) is a technique we have developed to deliver genes into individual cells within intact tissues, although it may also be applicable to cells in disperse
实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate






