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- 库存:
99999
- 规格:
10×100 μL
产品简介:本产品专为腺病毒质粒的高效构建而设计,其基因型经过优化,显著提升重组效率和操作便捷性。JM108 菌株缺失核酸内切酶 (endA),提高了质粒 DNA 的产量和质量;重组酶缺陷型(recA)减少插入片段的同源重组概率,保证了插入 DNA 的稳定性;菌株预装腺病毒骨架质粒 pAdEasy-1 (AmpR),无需额外转化步骤,只需转入线性化目的片段即可完成同源重组,大幅简化实验流程并提高重组效率;该菌株具有链霉素抗性 (StrR)。本产品经优化的感受态制备工艺制备而成,使用pUC19 质粒,检测转化效率>0.5×10 ⁹cfu/μg DNA。
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文献和实验Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
Transforming chemically competent cells
: According to the original TSS paper and qualitative experience (JM), this step is completely optional and may actually reduce transformation efficiency. Incubate cells on ice for 2 min. Add 1 mL SOC (2XYT and LB are also suitable, original paper suggests
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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