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- 详细信息
- 文献和实验
- 技术资料
- 规格:
96×100 μL
产品应用:
♦ 适用于动物细胞转染级质粒制备;
♦ 黏粒文库构建与扩增;
♦ 高保真克隆载体生产;
♦ 需要高纯度超螺旋质粒的实验体系。
产品特点:
♦ 遗传背景清晰工程菌株;
♦ 多重基因修饰显著提升质粒产量与结构稳定性。
(操作方法参考DLC201说明书)
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文献和实验Transforming chemically competent cells
: According to the original TSS paper and qualitative experience (JM), this step is completely optional and may actually reduce transformation efficiency. Incubate cells on ice for 2 min. Add 1 mL SOC (2XYT and LB are also suitable, original paper suggests
Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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