- ￥1350 - 3000
- Proteintech
- 68302-1-Ig
- 2025年10月09日
- IHC, Dot Blot, ELISA
- Mouse
- chemical compound
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
chemical compound
- 宿主：
Mouse
- 应用范围：
IHC, Dot Blot, ELISA
- 靶点：
m7G
- 抗体英文名：
m7G Monoclonal antibody
- 抗体名：
m7G Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive ELISA detected in | compound |
| Positive Dot Blot detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| DOT BLOT | DOT BLOT : 1:2500-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68302-1-Ig targets m7G in IHC, Dot Blot, ELISA applications and shows reactivity with chemical compound samples.
| 经测试应用 | IHC, Dot Blot, ELISA |
| 经测试反应性 | chemical compound |
| 文献引用反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | m7G |
| 别名 | 7-Methylguanosine |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2935381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
7-Methylguanosine (m7G) is a modified purine nucleoside. It is a methylated version of guanosine and when found in human urine, it may be a biomarker of some types of cancer. In the RNAs, 7-methylguanosine have been used to study and examine the reaction evolving methylguanosine. It also plays a role in mRNA as a blocking group at its 5´-end. The m7G modification actively participates in biological and pathological functions by affecting the metabolism of various RNA molecules, including messenger RNA, ribosomal RNA, microRNA, and transfer RNA. Increasing evidence indicates a critical role for m7G in human disease development, especially cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68302-1-IgDotBlot.pdf
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | Eur J Med Res A comprehensive analysis of m6A/m7G/m5C/m1A-related gene expression and immune infiltration in liver ischemia-reperfusion injury by integrating bioinformatics and machine learning algorithmsAuthors - Zhanzhi Meng |
117、CDw121a、CDw121b、CD126、CDw130、G-CSFR、PDGFR和VEGFR等。 图3-3 免疫分子命名的相互关系 (责任编辑：大汉昆仑王)
质沉淀，尤其是PI值在4.5附近的白蛋白，将这些杂蛋白用辛酸沉淀完毕后，再用法将抗体沉淀下来即可。 具体操作： （1）将腹水用0.06 M醋酸钠(pH4.0）按1：3稀释，在4 ℃条件下搅拌加入正辛酸，一般按每毫升未稀释的腹水加入40 ul正辛酸，然后4 ℃静置2 h; （2）4 ℃10000 g离心20min，可见辛酸因温度低而从系统中结晶析出漂浮在腹水上层，弃去此结晶以及底部沉淀，向上清中加入1/10体积的10×PBS（0.1M，pH 7.4),4 ℃条件下向其中加
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
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