- ￥1350 - 3000
- Proteintech
- 27921-1-AP
- 2025年10月09日
- WB, ELISA
- Rabbit
- Human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PIK3CA protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
PI3 Kinase p110 Alpha
- 抗体英文名：
PI3 Kinase p110 Alpha Polyclonal antibody
- 抗体名：
PI3 Kinase p110 Alpha Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse brain tissue, NIH/3T3 cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27921-1-AP targets PI3 Kinase p110 Alpha in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27539 Product name: Recombinant human PIK3CA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 345-550 aa of BC113601 Sequence: NVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| 别名 | p110 alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha |
| 计算分子量 | 1068 aa, 124 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC113601 |
| 基因名称 | PIK3CA |
| Gene ID (NCBI) | 5290 |
| RRID | AB_2918135 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42336 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | J Ethnopharmacol Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.Authors - Shi Dong |
| mouse,human | WB | Heliyon Anticancer potential of grifolin in lung cancer treatment through PI3K/AKT pathway inhibitionAuthors - Li Wang |
| mouse | WB | JCI Insight Regulatory T-cell and neutrophil extracellular traps interaction contributes to the development of immunosuppression in sepsisAuthors - Yuxin Shi |
| human | WB | Anticancer Drugs STK214947, a novel indole alkaloids, inhibits HeLa and SK-HEP-1 cells survival and EMT process by blocking the Notch3 and Akt signalsAuthors - Zihan Xu |
| human | WB | J Immunother Vitexin Inhibits TNBC Progression and Metastasis by Modulating Macrophage Polarization Through EGFR SignalingAuthors - Yufeng Lin |
| mouse | WB | Eur J Pharmacol Novel Applications of Metformin in the Treatment of Septic Myocardial Injury Based on Metabolomics and Network PharmacologyAuthors - Xingyu Li |
的I型PI3K. 哺乳动物 细胞中Ι型PI3K又分为IA和IB两个亚型, 他们分别从酪氨酸激酶连接受体和G蛋白连接受体传递信号.IA 型PI3K是由催化亚单位p110和调节亚单位p85所组成的二聚体蛋白, 具有类脂激酶和蛋白激酶的双重活性.PI3K通过两种方式激活, 一种是与具有磷酸化酪氨酸残基的生长因子受体或连接蛋白相互作用, 引起二聚体构象改变而被激活; 另一种是通过Ras和p110直接结合导致PI3K的活化. PI3K激活的结果是在质膜上产生第二信使PIP3, PIP3与细胞内含有PH结构
存在于胞质中，等待适当的信号激活。P13K通过两种方式激活：一种是与具有磷酸化酪氨酸残基的生长因子受体或连接蛋白相互作用，引起二聚体构象改变而被激活。对RTK而言，这种信号来自于配体介导的激酶的活化，结果使位于细胞膜内表面的酪氨酸残基磷酸化。磷酸化的酪氨酸残基立即成为细胞内信号蛋白的结合位点，通过与P85的SH2结构域结合而把P85―P110复合物聚集到细胞膜上并使之活化。另一种是通过Ras和Pll0直接结合，导致P13K的活化。PI3K被激活后，在细胞膜上生成第二信使PIPa，PIPa与细胞内含有PH
tyrosine kinases (RTKs) and the concomitant assembly of receptor–PI3K complexes. These complexes localize at the membrane where the p110 subunit of PI3K catalyses the conversion of PtdIns(4,5)P2 (PIP2) to PtdIns(3,4,5)P3 (PIP3). PIP3 serves as a second
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