- ￥1350 - 3000
- Proteintech
- 68320-1-Ig
- 2026年05月27日
- WB, IHC, ELISA
- Mouse
- human, mouse, rat, pig, rabbit
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human COL3A1 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit
- 宿主：
Mouse
- 应用范围：
WB, IHC, ELISA
- 靶点：
Collagen Type III
- 抗体英文名：
Collagen Type III Monoclonal antibody
- 抗体名：
Collagen Type III Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | pig colon tissue, human placenta tissue, human rectum tissue, rabbit large intestine tissue, rat colon tissue |
| Positive IHC detected in | human ovary tumor tissue, human hepatocirrhosis tissue, human placenta tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68320-1-Ig targets Collagen Type III in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag4229 Product name: Source: e coli.-derived, PET28a Tag: 6*His Domain: of Sequence: |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type III, alpha 1 |
| 别名 | COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain |
| 计算分子量 | 1466 aa, 139 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC028178 |
| 基因名称 | COL3A1 |
| Gene ID (NCBI) | 1281 |
| RRID | AB_2935393 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02461 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).
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文献和实验Recombinant Collagen Trimers from Insect Cells and Yeast
have been unsuccessful, but active recombinant human prolyl 4-hydroxylase has been produced in insect cells and yeast by coexpression of its α- and β-subunits (7 ,7 ). We have recently shown that recombinant human type III collagen with a stable triple helix
they may be split 1:4 into fresh media. (Fungizone can be omitted from this point onwards). 2. Stage I - Preparation of collagen I from rat tails Note: Protocol for approximately 12-14 adolescent (fresh or frozen) rat tails 1) Prepare rat
primer: 5'-CTGACCTGTCTCCATGTTGCA-3'; ------ Ccollagen type III--- GCGGCTTTTCACCATATTACG CGTTAACAGACTTGAGTGAAGACATGA collagen type III----forward primer: 5'-TGCCATTGCTGGAGTTGGA-3'; reverse primer: 5'-GAAGACATGATCTCCTCAGTGTTGA-3'; ----- rGlut4----
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