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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human COL3A1 protein
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat, pig, rabbit
- 宿主:
Mouse
- 应用范围:
WB, IHC, Indirect ELISA
- 靶点:
Collagen Type III
- 抗体英文名:
Collagen Type III Monoclonal antibody, PBS Only
- 抗体名:
Collagen Type III Monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
68320-1-PBS targets Collagen Type III in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IHC, Indirect ELISA |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag4229 Product name: Source: e coli.-derived, PET28a Tag: 6*His Domain: of Sequence: |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type III, alpha 1 |
| 别名 | COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain |
| 计算分子量 | 1466 aa, 139 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC028178 |
| 基因名称 | COL3A1 |
| Gene ID (NCBI) | 1281 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02461 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).
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文献和实验Dilute to 4% in PBS prior to use Screens for cd-1, size 40 mesh Sigma S07707-5EA Stainless steel grids, 5 per pack
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
signals on the chromosomes. As such, a corresponding control experiment should always be included. iii. Rinse slides in PBS and place in rack. Wash slides in wash solution 1 for 15 min and then in wash solution 2 for 15 min
Biological coating procedures for glass coverslips
COLLAGEN Collagen may be used to coat glass coverslips for the growth of epithelial, endothelial and muscle cells, neurons, PC12 and CHO cell lines. Type I collagen is most often used for coating substrates for cell culture
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