- ￥2800
- Proteintech
- CL488-80596
- 2025年10月09日
- IF/ICC, FC (Intra)
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, rat
- 宿主：
Rabbit
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
Phospho-mTOR (Ser2448)
- 抗体英文名：
CoraLite® Plus 488-conjugated Phospho-mTOR (Ser2448) Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Phospho-mTOR (Ser2448) Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | PMA treated HEK-293 cells |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-80596 targets Phospho-mTOR (Ser2448) in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 12-rapamycin associated protein 1 |
| 别名 | FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein |
| 计算分子量 | 289 kDa |
| 观测分子量 | 250-289 kDa |
| GenBank蛋白编号 | BC117166 |
| 基因名称 | mTOR |
| Gene ID (NCBI) | 2475 |
| RRID | AB_2934630 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42345 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.
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文献和实验TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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