- ￥1350 - 3000
- Proteintech
- 26156-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant mouse IL-2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
IL-2
- 抗体英文名：
IL-2 Polyclonal antibody
- 抗体名：
IL-2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
| Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26156-1-AP targets IL-2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, Cell treatment |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag24079 Product name: Recombinant mouse IL-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-169 aa of NM-008366 Sequence: APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 2 |
| 别名 | Il2, Il 2, interleukin 2, Interleukin-2, T-cell growth factor |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | NM-008366 |
| 基因名称 | IL-2 |
| Gene ID (NCBI) | 16183 |
| RRID | AB_2880407 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04351 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the calculated molecular weight of IL2?
19 kDa
Where is IL-2 produced?
IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.
What allows the protein to bind to its receptor?
The N-terminal 20 amino acids are essential for protein-receptor interaction.
What are the post-translational modifications of IL-2?
IL-2 may present glycosylation, yet the function of this modification has not been confirmed.
What is the role of IL-2?
IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion.
What activates the IL-2 pathway?
Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway.
How does IL-2 expression change during an immune response?
During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells.
How do the responses to strong and weak IL-2 signals differ?
Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells.
What negatively regulates IL-2?
Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.
(PMIDs: 22343569, 24376444, 15473953)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | IF | Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopyAuthors - Aleksandra Klimas |
| human | Invest New Drugs A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.Authors - Weihua Hou | |
| human | IHC | J Cancer IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.Authors - Jia-Qian Hu |
| human | WB | Peptides Synthesis of potent antagonists of receptors for growth hormone-releasing hormone with antitumor and anti-inflammatory activity.Authors - Renzhi Cai |
| mouse | WB | Lipids Health Dis Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway.Authors - Ziying Shen |
| human | WB | Lung Anti-inflammatory Properties of the Alpha-Melanocyte-Stimulating Hormone in Models of Granulomatous Inflammation.Authors - Abdolrazagh Hashemi Shahraki |
斑点杂交法测定培养细胞IL-2 mRNA的含量免疫测定 【试剂与仪器】 地高辛标记和检测试剂盒（Boerhinger Mannheim，No1093657） 【操作步骤】 （1） 杂交后彻底清洗，将膜置入清洗缓冲液中1～5min； （2） 100ml封闭缓冲液（1×）中封闭30min； （3） 抗地高辛-AP复合物（75mu/ml）用封闭缓冲液（1×）1：10000稀释
列为5`-TGACTCA-3`）。TRE命名是因为TPA可通过活化PKC而诱导AP-1并与AP-1结合位点结合。 IL-2基因在转录起始部位的5`端含有一个增强子，它仅存在T细胞中以细胞特异性的方式控制IL-2基因的转录，几种结合到IL-2基因增强子区域的DNA结合蛋白被认为是MHC/Ag结合TCR与IL-2基因转录调节之间重要的联系环节。这个增强子中的一些序列可以特异性地同一个称为AP-1的转录激活蛋白相结合。AP-1可存在于不同细胞类型中，许多不同基因的调节区域
网络 一、免疫学检测法 免疫学检测法的基本原理是细胞因子（或受体）与相应的特异性抗体（单克隆抗体或多克隆抗体）结合，通过同位素、荧光或酶等标记技术加以放大和显示，从而定性或定量显示细胞因子（或受体）的水平。这类方法的优点是实验周期短，少受抑制物或相似生物池功能因子的干扰，如抗体的特异性高可区分不同型或亚型的细胞因子（如IFN），一次能检测大量标本，易标准化。与生物
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