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100ul/1ml
| 规格: | 100ul | 产品价格: | ¥480.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥3600.0 |
| 产品编号 | bs-0295D-BF594 |
| 英文名称 | Donkey Anti-Rabbit IgG H&L, BF594 conjugated |
| 中文名称 | BF594标记的驴抗兔IgG H&L |
| 英文别名 | Donkey Anti-Rabbit IgG H&L (BF594); Immunoglobulin G; |
| 产品应用 | IF=1:100-1000, ICC/IF=1:100-1000, Flow-Cyt=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat, Rabbit |
| 抗体来源 | Donkey |
| 免疫原 | Native rabbit IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 克隆类型 | Polyclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |




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文献和实验XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂:PBS(磷酸盐缓冲液pH7.4)(实验室常规配置);非免疫山羊血清;Triton X-100(sigma);BSA抗体稀释液(实验室常规配置);A蛋白抗体(Santa cruz);B蛋白抗体(Abcam);Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen);Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
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