Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-20633R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-20633R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Haptoglobin beta Rabbit pAb(bs-9888R)-50ul/100ul/200ul
￥1180
phospho-TBK1 (Ser172) Recombinant Rabbit mAb(bsm-60659R)-50ul/100ul/25ul
￥800LRP5L Rabbit pAb(bs-11590R)-50ul/100ul/200ul
￥1180
Ku70 / XRCC6 Rabbit pAb, RBITC conjugated(bs-2295R-RBITC)-100ul
￥2980IFI16 Rabbit pAb, AP conjugated(bs-15548R-AP)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20633R |
| 英文名称 | HMGB1 Rabbit pAb |
| 中文名称 | 高迁移率族蛋白B1抗体 |
| 英文别名 | High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat, 肾, 肾, 肾, 肾, 肾, 肾, 肾 (Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse HMGB1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 25 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Histones > HMGs Epigenetics and Nuclear Signaling > Transcription > Domain Families > HMG Box Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus Signal Transduction > Antibodies > hmgb1 |
| 亚基 | Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. |
| 亚细胞定位 | Nucleus. Chromosome. |
| 相似性 | Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. |
| 功能 | DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
This image was generously provided by Juanli Fu, at Southwest University in Chong Qing, China. 4% Paraformaldehyde fixed PC12 cells stained with Rabbit Anti- HMGB1 Polyclonal Antibody (bs-20633R) at 1:300 for 3 hours at 4°C, followed by Rhodamine-conjugated secondary antibody for an additional hour.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HMGB1) Polyclonal Antibody, Unconjugated (bs-20633R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 5.714 }}] {Gao R et al. Quasi-ultrafine particles promote cell metastasis via HMGB1-mediated cancer cell adhesion. Environ Pollut. 2019 Oct 23:113390.} {WB} {Human}
[IF={{ 3.3 }}] {Xiaohua Zhang. et al. Pseudorabies Virus Infection Activates the NLRP3 and IFI16 Inflammasomes to Trigger Pyroptosis. VET MICROBIOL. 2023 Jun;:109826} {WB} {Pig}
[IF={{ 2.7 }}] {Wenyue Qiu. et al. Tert-butylhydroquinone attenuates LPS-induced pyroptosis of IPEC-J2 cells via downregulating HMGB1/TLR4/NF-κB axis. J ANIM PHYSIOL AN N. 2023 Sep;:} {WB} {Porcine}
[IF={{ 12.1 }}] {Xianquan Feng. et al. A Self-Amplifying Photodynamic Biomedicine for Cascade Immune Activation Against Triple-Negative Breast Cancer. SMALL. 2025 Jan;:2410214} {IF} {Mouse}
[IF={{ 11.3 }}] {Hai Wang. et al. Photoirradiation- and Extracellular-Matrix-Encouraged Photon-Transition Switching Regulates Spatiotemporal Sequential Photoimmunotherapy. SMALL STRUCT. 2025 Jan;:2400574} {IF} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
/96T 2000/3000 进口分装 F0021 骨成型蛋白-9(BMP-9) ELISA 48T/96T 2000/3000 进口分装 F0022 BMP-R1A ELISA 48T/96T 2000/3000 进口分装 F0023 BMP-R2 ELISA 48T/96T 2000/3000 进口分装 F0024 BTC ELISA 48T/96T 2000/3100 进口分装 F0030 CAE-Ab ELISA 96
/96T 2000/3000 进口分装 F0021 骨成型蛋白-9(BMP-9) ELISA 48T/96T 2000/3000 进口分装 F0022 BMP-R1A ELISA 48T/96T 2000/3000 进口分装 F0023 BMP-R2 ELISA 48T/96T 2000/3000 进口分装 F0024 BTC ELISA 48T/96T 2000/3100 进口分装 F0030 CAE-Ab ELISA 96
技术资料暂无技术资料 索取技术资料
文献支持
HMGB1 Rabbit pAb(bs-20633R)-50ul/100ul/200ul
￥1180 - 2800
