- ￥980 - 4800
- Bioss
- bs-0295D
- 2025年10月10日
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1mg/10mg
| 规格： | 1mg | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥4800.0 |
| 产品编号 | bs-0295D |
| 英文名称 | Donkey Anti-Rabbit IgG H&L |
| 中文名称 | 驴抗兔IgG H&L |
| 英文别名 | Rabbit Immunoglobulin G; Rb IgG; |
| 产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:200-1000, IF, ELISA=1:2000-10000 Not yet tested in other applications. |
| 交叉反应 | Rabbit |
| 抗体来源 | Donkey |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
| {ELISA} | {1:2000-10000} |
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文献和实验[IF={{ 9.9 }}] {Lijun You. et al. Magnetic polyphosphazene@Au particles as substrates for multiple-detection of immunoproteins by surface-enhanced Raman spectroscopy. J COLLOID INTERF SCI. 2023 Oct;648:1006} {Other} {}
[IF={{ 6.304 }}] {Ye Fenget al. Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis . 2020 Jul 8;11(7):513.} {IF} {mouse}
[IF={{ 5.3 }}] {Ennio Avolio. et al. Vasostatin-1 restores autistic disorders in an idiopathic autism model (BTBR T+ Itpr3tf/J mice) by decreasing hippocampal neuroinflammation. PROG NEURO-PSYCHOPH. 2024 Dec;135:111131} {WB} {Mouse}
[IF={{ 4.8 }}] {Lamei Li. et al. Peripheral CCL2 induces inflammatory pain via regulation of Ih currents in small diameter DRG neurons. FRONT MOL NEUROSCI. 2023; 16: 1144614} {WB} {Mouse}
[IF={{ 2.1 }}] {Chunyu Jiang. et al. AMD3100 and SDF‑1 regulate cellular functions of endothelial progenitor cells and accelerate endothelial regeneration in a rat carotid artery injury model. Mol Med Rep. 2020 Oct;22(4):3201-3212} {WB} {Human}
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
对照。 二、注意事项 1、荧光染色后一般在 1h 内完成观察，或于 4℃ 保存 4h ，时间过长，可能会使荧光提前衰退。 2、每次试验均需设置以下三种对照： （1） 阳性对照：阳性血清+荧光标记物； （2）阴性对照：阴性血清+荧光标记物； （3）荧光标记物对照：PBS +荧光标记物。 三、免疫荧光双标的经验之谈 1、选取一抗时，要求来源于两种不同的动物，我用的是来源于家兔和大鼠的抗体，二抗则是不同荧光信号标记的，我用的是 donkey anti-rabbit-FITC（绿）和donkey anti
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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