Gibco 31800-022 RPMI1640干粉培养基

 **产品名称**：Gibco™ RPMI 1640 干粉培养基

**货号**：31800-022

**品牌**：Gibco（Thermo Fisher Scientific 旗下） 

**形式**：粉末 **规格**：10 × 1 L 包装

主要成分 - 含：**L-谷氨酰胺、酚红、葡萄糖、谷胱甘肽、高浓度维生素（生物素、B12、PABA、肌醇、胆碱）、氨基酸、无机盐** - 不含：**HEPES、碳酸氢钠、丙tong酸钠** - 缓冲体系：需额外添加碳酸氢钠（建议 2.0 g/L），依赖 5%–10% CO₂ 维持 pH - 渗透压：260–310 mOsm/kg（配制后） - pH：7.2–7.4（5% CO₂ 平衡后）

*经典配方**：由 Roswell Park Memorial Institute 研发，**适配悬浮/单层培养人白血病细胞及多种哺乳动物细胞**。 - **高维生素+谷胱甘肽**：含 EMEM/DMEM 所不含的生物素、B12、PABA；高浓度肌醇、胆碱；**谷胱甘肽提供抗氧化保护**，降低细胞应激。 - **干粉剂型**：**保质期长、运输方便、成本更低**，适合实验室长期大量使用。 - **无 HEPES、无碳酸氢钠**：可**灵活调整缓冲体系**，适合特殊培养条件或需严格控制离子浓度的实验。 - **低内毒素**：≤0.03 EU/mL，**支原体/细菌/真菌阴性**，支持敏感细胞与功能实验。

适用细胞 - 悬浮细胞：**Jurkat、HL-60、K562、PBMC、白血病细胞**。 - 贴壁细胞：**HeLa、MCF-7、PC12、星形胶质细胞、肿瘤细胞系**。

应用场景：**肿瘤细胞培养、免疫细胞（T/B 细胞）、杂交瘤/单抗制备、病毒包装、原代细胞**。

 质量与储存 - 无菌：粉末无菌，配制后建议 0.22 μm 过滤灭菌。

- 保质期：**36 个月（未开封，2–8°C）**。

 - 储存：**2–8°C 避光干燥**；室温运输，**防潮、勿冷冻**。

- 用途：**科研用，不可用于人体/动物给药或临床诊断**。

**Product Name**：Gibco™ RPMI 1640 Medium, powder **Catalog No.**：31800-022 **Brand**：Gibco (Thermo Fisher Scientific) **Form**：Powder **Size**：10 × 1 L packs #### Key Components - **With**：L-glutamine, phenol red, D-glucose, glutathione, high levels of vitamins (biotin, B12, PABA, inositol, choline), amino acids, inorganic salts - **Without**：HEPES, sodium bicarbonate, sodium pyruvate - Buffering：Requires sodium bicarbonate (2.0 g/L recommended); relies on 5%–10% CO₂ for pH stability - Osmolality：260–310 mOsm/kg (after reconstitution) - pH：7.2–7.4 (equilibrated with 5% CO₂) #### Features - **Classic formulation**：Developed at Roswell Park Memorial Institute; **optimized for human leukemic cells (suspension & monolayer)**；suitable for diverse mammalian cells. - **High vitamins + glutathione**：Contains biotin, B12, PABA (absent in EMEM/DMEM); high inositol/choline; **glutathione provides antioxidant protection** to reduce cellular stress. - **Powder format**：**Long shelf life, easy shipping, cost-effective**；ideal for long-term, high-volume laboratory use. - **HEPES-free, sodium bicarbonate-free**：Allows **flexible buffer adjustment**；suitable for specialized culture conditions or strict ion control experiments. - **Low endotoxin**：≤0.03 EU/mL; **mycoplasma/bacteria/fungus-free**；supports sensitive cells and functional assays. #### Recommended Cells - Suspension cells：**Jurkat, HL-60, K562, PBMC, leukemic cells**. - Adherent cells：**HeLa, MCF-7, PC12, astrocytes, carcinoma cell lines**. - Applications：**Tumor cell culture, immune cells (T/B cells), hybridoma/antibody production, viral packaging, primary cells**. #### Quality & Storage - Sterility：Sterile powder; filter-sterilize (0.22 μm) after reconstitution. - Shelf life：**36 months (unopened, 2–8°C)**. - Storage：**2–8°C, dry and protected from light**；ship at ambient temperature; **keep dry, do not freeze**. - Intended use：**For research only. Not for human/animal administration or clinical diagnostics.** -