产品名称：Gibco DMEM（高糖）培养基Gibco 12100046
品牌：invitrogen（Gibco）
规格：10*1L（即一盒10小袋，每袋可配1L）
货号：12100046
成分：含： 4500 mg/L D-葡萄糖和 L-谷氨酰胺
不含：b酮酸钠和碳酸氢钠
保存温度：2-8℃
有效期：3年（36个月）
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
相关 Gibco DMEM（高糖）培养基Gibco 12100046 Gibco培养基有：
12430054 高糖DMEM，含HEPES，不含b酮酸钠 500mL 询价
11965092 高糖DMEM，不含HEPES，不含b酮酸钠 500mL 询价
11966025 无糖DMEM，不含b酮酸钠 500mL 询价
11995065 高糖DMEM，含b酮酸钠 500mL 询价
12100046 高糖DMEM，不含b酮酸钠 10×1L 询价
12800017 高糖DMEM，含b酮酸钠 10×1L 询价
12800082 高糖DMEM，含b酮酸钠 1×10L 询价
31600034 低糖DMEM 10×1L 询价
31600083 低糖DMEM 1×10L 询价
11095080 MEM Earles，不含非必需氨基酸 500mL 询价
61100061 MEM Earles，不含非必需氨基酸 10×1L 询价
41500034 MEM，含非必需氨基酸 10×1L 询价
12440046 IMDM，含碳酸氢钠 1000mL 询价
12200036 IMDM，不含碳酸氢钠 10×1L 询价
11330032 DMEM/F-12，含HEPES 500mL 询价
12400024 DMEM/F-12，含HEPES 10×1L 询价
12500062 DMEM/F-12，不含HEPES 10×1L 询价
21700075 F-12 Nutrient Mixture 10×1L 询价
10902088 Sf-900 II SFM 1000mL 询价
12491015 Advanced DMEM 500mL 询价
11875093 RPMI 1640 500mL 询价
22400089 RPMI 1640，含HEPES 500mL 询价
31800022 RPMI 1640 10×1L 询价
23400021 RPMI 1640，含HEPES 10×1L 询价
12633012 Advanced RPMI 1640 500mL 询价
11900024 α-MEM，含核糖核苷和脱氧核糖核苷 10×1L 询价
12000022 α-MEM，不含核糖核苷和脱氧核糖核苷 10×1L 询价
主营品牌：Sigma、Amresco、santa、hyclone、PeproTech、ABcam、life、Axygen、Corning、AMEKO、Merck、promega、GE、nunc等
主营产品:
试剂盒：ELISA试剂盒、免疫组化试剂盒、分子生物学试剂盒、细胞凋亡试剂盒
细胞培养：血清，专业培养基，常用传统培养基
耗材：进口、国产细胞培养耗材、普通实验耗材
生化试剂：原装进口生化试剂、进口分装生化试剂、国产生化试剂
抗体：进口原装抗体、进口分装抗体、单克隆抗体、多克隆抗体、一抗、二抗等
