产品名称:Gibco DMEM(高糖)培养基Gibco 12100046
品牌:invitrogen(Gibco)
规格:10*1L(即一盒10小袋,每袋可配1L)
货号:12100046
成分:含: 4500 mg/L D-葡萄糖和 L-谷氨酰胺
不含:b酮酸钠和碳酸氢钠
保存温度:2-8℃
有效期:3年(36个月)
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With | Without |
• High Glucose | • Sodium Pyruvate |
• L-glutamine | • HEPES |
• Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
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主营品牌:Sigma、Amresco、santa、hyclone、PeproTech、ABcam、life、Axygen、Corning、AMEKO、Merck、promega、GE、nunc等
主营产品:
试剂盒:ELISA试剂盒、免疫组化试剂盒、分子生物学试剂盒、细胞凋亡试剂盒
细胞培养:血清,专业培养基,常用传统培养基
耗材:进口、国产细胞培养耗材、普通实验耗材
生化试剂:原装进口生化试剂、进口分装生化试剂、国产生化试剂
抗体:进口原装抗体、进口分装抗体、单克隆抗体、多克隆抗体、一抗、二抗等