Biosharp白鲨 HT培养基补充物(50×) BL317

Biosharp（白鲨）HT 培养基补充物 (50×) 货号：BL317A

中文产品介绍

产品简介

HT 培养基补充物（50×）为无菌液体浓缩添加剂，有效成分为次黄嘌呤（Hypoxanthine）、胸苷（Thymidine）。50 倍储存液，按照 1:50 比例加入基础培养基稀释至 1× 工作浓度；终浓度：次黄嘌呤 100 μM，胸苷 16 μM白鲨。经过 0.22 μm 过滤除菌，用于杂交瘤融合之后 HAT 筛选结束阶段，撤除氨基蝶呤后杂交瘤细胞维持培养，支持单克隆抗体杂交瘤细胞生长。

• 货号：BL317A
• 规格：10 mL / 瓶（可配制 500 mL 1× 完全培养基）
• 组分：次黄嘌呤、胸苷，无菌水溶液
• 储存条件：‑20 ℃避光保存，避免反复冻融白鲨

主要特点

1. 即用型无菌 50× 浓缩液，无需自行称量溶解，直接按 1 mL 补充物加入 50 mL 培养基配比使用。
2. 严格质控：无菌检测合格，降低杂交瘤污染风险；批次稳定，保障杂交瘤细胞存活增殖。
3. 应用时序：HAT 选择培养基培养结束后使用，去掉氨基蝶呤，添加 HT 补充物维持杂交瘤代谢通路，淘汰未融合骨髓瘤细胞残余，用于单克隆抗体制备。

下游应用

杂交瘤细胞 HT 阶段维持培养；单克隆抗体制备；B 细胞融合后续筛选培养；部分 DHFR 缺陷细胞株筛选培养。

储存与注意事项

‑20 ℃避光，避免反复冻融；无菌条件下操作；本产品仅科研使用，不可用于临床诊断。

⚠️提示：HT 不含氨基蝶呤，不能替代 HAT，HAT 是筛选阶段，HT 是筛选完成后的维持阶段。

English Product Introduction

Product Description

HT Media Supplement (50×), Cat.No.BL317A (Biosharp) is a sterile liquid concentrated supplement containing hypoxanthine and thymidine. Dilute 50‑fold with basal cell‑culture medium to obtain 1× working solution. Final working concentration: hypoxanthine 100 μM, thymidine 16 μM白鲨. Sterilized by 0.22 μm filtration. It is applied after HAT selection, once aminopterin is removed, to sustain the growth of hybridoma cells for monoclonal‑antibody production.

• Catalog No.: BL317A
• Size: 10 mL per vial (sufficient for preparing 500 mL 1× complete medium)
• Composition: Hypoxanthine, thymidine in sterile aqueous solution
• Storage: Store at −20 ℃ protected from light. Avoid repeated freeze‑thaw cycles白鲨

Key Features

1. Ready‑to‑use sterile 50× liquid stock. No powder weighing or dissolution required. Mix 1 mL supplement with 50 mL basal medium to reach 1× concentration.
2. Rigorous sterility QC to minimize contamination risk for hybridoma culture. Consistent batch‑to‑batch performance supports hybridoma survival and proliferation.
3. Workflow hint: used subsequent to HAT selection. After removing aminopterin, HT rescues nucleotide‑salvage pathway of hybridoma cells for mAb generation.

Applications

Hybridoma maintenance after HAT selection; monoclonal‑antibody production; post‑fusion B‑cell culture; DHFR‑deficient cell line selection.

Storage & Notes

Store at −20 ℃ in dark; avoid repeated freeze‑thaw. Aseptic manipulation required. For research‑only, not for clinical diagnostics.

Note: HT contains no aminopterin and cannot replace HAT. HAT for selection; HT for post‑selection maintenance.