GibcoRPMI 11875-093 1640培养基

 **产品名称**：Gibco™ RPMI 1640 液体培养基（含L-谷氨酰胺，无HEPES）

**货号**：11875-093

**品牌**：Gibco（Thermo Fisher Scientific 旗下）

**形式**：无菌液体 **规格**：500 mL/瓶 #### 主要成分 - 含：**L-谷氨酰胺、酚红、葡萄糖、谷胱甘肽、高浓度维生素（生物素、B12、PABA、肌醇、胆碱）、氨基酸、无机盐** - 不含：**HEPES、丙tong酸钠** - 缓冲体系：碳酸氢钠（2.0 g/L），依赖5%–10% CO₂维持pH - 渗透压：260–310 mOsm/kg - pH：7.2–7.4（5% CO₂平衡）

**经典配方**：由Roswell Park Memorial Institute研发，**专为悬浮/单层培养人白血病细胞设计**，广泛适配多种哺乳动物细胞。 - **高维生素+谷胱甘肽**：含EMEM/DMEM所不含的生物素、B12、PABA；高浓度肌醇、胆碱；**谷胱甘肽提供抗氧化保护**，降低细胞应激。 - **无HEPES**：标准碳酸氢钠缓冲，适合**常规培养、悬浮细胞、长期实验**，避免HEPES潜在毒性。 - **即开即用**：无菌过滤，含谷氨酰胺，**无需配制/调pH/过滤**，减少批次差异。 - **低内毒素**：≤0.03 EU/mL，**支原体/细菌/真菌阴性**，支持敏感细胞与功能实验。 #### 适用细胞 - 悬浮细胞：**Jurkat、HL-60、K562、PBMC、白血病细胞**。 - 贴壁细胞：**HeLa、MCF-7、PC12、星形胶质细胞、肿瘤细胞系**。

- 应用场景：**肿瘤细胞培养、免疫细胞（T/B细胞）、杂交瘤/单抗制备、病毒包装、原代细胞**。

质量与储存 - 无菌：0.1 μm过滤灭菌，**无支原体、细菌、真菌**。

- 保质期：**12个月（未开封，2–8°C）**。

- 储存：**2–8°C避光**；室温运输，**严禁冷冻**。

- 用途：**科研用，不可用于人体/动物给药或临床诊断**。 

**Product Name**：Gibco™ RPMI 1640 Medium, liquid, with L-glutamine, without HEPES **Catalog No.**：11875-093 **Brand**：Gibco (Thermo Fisher Scientific) **Form**：Sterile liquid **Size**：500 mL/bottle #### Key Components - **With**：L-glutamine, phenol red, D-glucose, glutathione, high levels of vitamins (biotin, B12, PABA, inositol, choline), amino acids, inorganic salts - **Without**：HEPES, sodium pyruvate - Buffering：Sodium bicarbonate (2.0 g/L); requires 5%–10% CO₂ for pH stability - Osmolality：260–310 mOsm/kg - pH：7.2–7.4 (equilibrated with 5% CO₂) #### Features - **Classic formulation**：Developed at Roswell Park Memorial Institute; **optimized for human leukemic cells (suspension & monolayer)**；suitable for diverse mammalian cells. - **High vitamins + glutathione**：Contains biotin, B12, PABA (absent in EMEM/DMEM); high inositol/choline; **glutathione provides antioxidant protection** to reduce cellular stress. - **HEPES-free**：Standard bicarbonate buffer; ideal for **routine culture, suspension cells, long-term experiments**; avoids HEPES-related cytotoxicity. - **Ready-to-use**：Sterile-filtered; pre-supplemented with L-glutamine; **no preparation/pH adjustment/filtration needed**; minimizes lot-to-lot variation. - **Low endotoxin**：≤0.03 EU/mL; **mycoplasma/bacteria/fungus-free**; supports sensitive cells and functional assays. #### Recommended Cells - Suspension cells：**Jurkat, HL-60, K562, PBMC, leukemic cells**. - Adherent cells：**HeLa, MCF-7, PC12, astrocytes, carcinoma cell lines**. - Applications：**Tumor cell culture, immune cells (T/B cells), hybridoma/antibody production, viral packaging, primary cells**. #### Quality & Storage - Sterility：0.1 μm filter-sterilized; **mycoplasma, bacteria, and fungus-free**. - Shelf life：**12 months (unopened, 2–8°C)**. - Storage：**2–8°C, protected from light**; ship at ambient temperature; **do not freeze**. - Intended use：**For research only. Not for human/animal administration or clinical diagnostics.**