Biosharp白鲨 BL3301B 293细胞无血清培养基

产品简介
BL3301B 是一款化学成分确定、无血清、无动物源成分（AOF）的专用培养基，适配 HEK293 系列细胞（293F、293S、293T 等）的高密度悬浮培养、高效瞬时转染与重组蛋白 / 病毒载体大规模生产，无需血清或额外添加剂，可直接用于工业级生物药上游培养白鲨。
产品参数
形态：红色澄清液体，即用型
适用细胞：HEK293 各亚型（293F、293S、293T、Expi-293F 等）
成分特点：无血清、无动物源、无外源蛋白 / 生长因子
pH 值：7.0–7.4
渗透压：275±15 mOsm/kg
无菌 / 支原体：无菌、无支原体
内毒素：＜5 EU/mL
培养环境：37℃、5% CO₂、饱和湿度
保存条件：2–8℃避光
有效期：12 个月
核心特点
高密度悬浮培养：支持 293 细胞密度达 2×10⁶–5×10⁶ cells/mL，活率＞95%。
转染效率高：适配 PEI、脂质体等转染试剂，瞬时转染效率＞90%，重组蛋白 / 病毒滴度高。
无动物源、低批次差异：完全无血清、无动物成分，批次稳定性强、质控严格，适合 GMP 合规生产。
支持长期传代：可连续传代 20 代以上，细胞形态、增殖与转染性能稳定。
应用场景
重组蛋白（抗体、酶、细胞因子）表达
病毒载体（AAV、慢病毒、腺病毒）包装与生产
瞬时转染高通量筛选
293 细胞基础研究与信号通路分析
使用要点
直接使用：无需添加血清或生长因子，可直接用于细胞复苏、传代与转染。
接种密度：悬浮培养接种密度 3×10⁵–5×10⁵ cells/mL，每 2–3 天补料或传代。
转染建议：转染前 24 h 调整细胞密度至 1×10⁶ cells/mL，转染时细胞活率＞95%。
注意事项
仅供科研与工业生产使用，不可用于临床诊断、治疗或人体给药。
无菌操作，避免微生物污染；开封后建议 1 个月内用完。
避免反复冻融，2–8℃避光保存。
English Product Introduction
Product Name：293 Cell Serum-Free Medium
Cat. No.：BL3301B
Brand：Biosharp
Size：1 L/bottle
Product Description
Biosharp BL3301B is a chemically defined, serum-free, animal-origin-free (AOF) medium specifically designed for high-density suspension culture, efficient transient transfection, and large-scale production of recombinant proteins/viral vectors using HEK293 cell lines (293F, 293S, 293T, etc.). It requires no serum or additional supplements and is ready-to-use for industrial biopharmaceutical upstream processes白鲨.
Specifications
Form：Red, clear liquid; ready-to-use
Target Cells：HEK293 variants (293F, 293S, 293T, Expi-293F, etc.)
Key Features：Serum-free, animal-origin-free, no exogenous proteins/growth factors
pH：7.0–7.4
Osmolarity：275±15 mOsm/kg
Sterility/Mycoplasma：Sterile, mycoplasma-free
Endotoxin：＜5 EU/mL
Culture Conditions：37°C, 5% CO₂, humidified atmosphere
Storage：2–8°C, protected from light
Shelf Life：12 months
Key Features
High-Density Suspension Culture：Supports cell densities of 2×10⁶–5×10⁶ cells/mL with viability＞95%.
High Transfection Efficiency：Compatible with PEI, liposomes, and other transfection reagents; transfection efficiency＞90% for high-titer recombinant protein/virus production.
AOF & Low Batch Variation：Completely serum-free and animal-component-free with strict quality control for consistent performance across batches.
Long-Term Subcultivation：Maintains stable cell morphology, proliferation, and transfection performance for ＞20 passages.
Applications
Recombinant protein (antibodies, enzymes, cytokines) expression
Viral vector (AAV, lentivirus, adenovirus) packaging & production
High-throughput transient transfection screening
Basic research and signaling pathway analysis in 293 cells
Recommended Protocol
Ready-to-Use：No serum or growth factors required; directly use for cell thawing, passaging, and transfection.
Seeding Density：3×10⁵–5×10⁵ cells/mL for suspension culture; feed or passage every 2–3 days.
Transfection：Adjust cell density to 1×10⁶ cells/mL 24 h before transfection; ensure viability＞95% at transfection.
Precautions
For research and industrial use only：Not for clinical diagnosis, therapy, or human administration.
Use aseptic technique to avoid contamination; use within 1 month after opening.
Do not freeze-thaw repeatedly; store at 2–8°C protected from light.