白鲨 减血清培养基BL1117B/BL1117A/BL152

Biosharp（白鲨）减血清培养基

货号区分：

• BL1117A：100 mL，含 L‑谷氨酰胺，不含酚红
• BL1117B：500 mL，含 L‑谷氨酰胺，不含酚红
• BL1524A：100 mL，含 L‑谷氨酰胺，含酚红
• BL1524B：500 mL，含 L‑谷氨酰胺，含酚红白鲨

中文产品介绍

产品简介

减血清培养基（Reduced Serum Medium）是以改良 MEM 为基础的即用型液体细胞培养基，添加 HEPES、碳酸氢钠缓冲体系，补充丙-酮酸钠、次黄嘌呤、胸苷、微量元素及生长辅助因子，可将血清用量降低约 50%，仍维持细胞正常增殖形态；0.22 μm 过滤除菌，经过无菌、支原体、内毒素检测，适用于 CHO‑K1、BHK‑21、HEK293、Sp2/0 等细胞系培养，适合蛋白表达、抗体制备、细胞扩增实验。

不含酚红版本（BL1117A/BL1117B）适合荧光检测、比色实验；含酚红版本（BL1524A/BL1524B）常规细胞传代培养使用。

货号规格对照表

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主要特点

1. 优化营养配方，添加多种生长辅助组分，血清添加量可降至 2‑5%，降低血清批次差异带来的实验波动，节约实验成本。
2. 预装 L‑谷氨酰胺、丙-酮酸钠、HEPES 缓冲，缓冲能力强，pH 环境稳定；即用型液体，无需自行配制。
3. 严格质控：无菌、支原体阴性、低内毒素，批次间一致性好，减少细胞应激、异常分化风险。
4. 两个版本可选：无酚红适配荧光、酶标检测；含酚红便于肉眼观察培养基 pH 状态。

下游应用

CHO、BHK‑21、HEK293、Sp2/0 等细胞的减血清扩增；重组蛋白、抗体表达培养；杂交瘤细胞培养；需要降低血清干扰的细胞功能实验。

储存条件

2‑8 ℃避光保存，避免反复冻融；开封后建议 1‑2 个月内用完；禁止‑20℃长期冻存。

⚠️提示：本产品为减血清，不是无血清培养基，仍需要补加一定比例血清使用。

English Product Introduction

Product Description

Reduced Serum Medium (Biosharp) is a ready‑to‑use liquid cell culture medium modified from MEM basal formula. Supplemented with HEPES, sodium bicarbonate buffer, sodium pyruvate, hypoxanthine, thymidine, trace elements and growth‑supporting factors. Serum supplementation can be reduced by approximately 50 % while supporting normal cell proliferation and morphology. Sterilized by 0.22 μm filtration, tested for sterility, mycoplasma and low endotoxin. Suitable for CHO‑K1, BHK‑21, HEK293, Sp2/0 and other cell lines for protein expression and cell expansion。

Phenol‑red‑free formats (BL1117A / BL1117B) are preferred for fluorescence and colorimetric assays; phenol‑red‑containing formats (BL1524A / BL1524B) for routine cell maintenance.

Catalog No. & Specification

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Key Features

1. Optimized nutrient formulation with multiple growth‑supporting additives. Working serum concentration can be lowered to 2‑5 %, reducing experimental variation caused by serum batch difference and lowering cost.
2. Pre‑supplemented with L‑glutamine, sodium pyruvate and HEPES for stable pH buffering. Ready‑to‑use liquid medium, no powder reconstitution required.
3. Rigorous quality‑control: sterile, mycoplasma‑free, low‑endotoxin. High batch‑to‑batch consistency to minimize cellular stress and aberrant differentiation.
4. Two formats available: phenol‑red‑free for fluorescence / microplate reading; phenol‑red‑containing for visual pH monitoring.

Applications

Reduced‑serum expansion of CHO, BHK‑21, HEK293, Sp2/0 cells; recombinant protein and antibody production; hybridoma cell culture; cell functional assays requiring lowered serum interference.

Storage

Store at 2‑8 ℃, protected from light. Avoid repeated freeze‑thaw. Use within 1‑2 months after opening. Do not store long‑term at −20 ℃.

Note: This is reduced‑serum medium, not serum‑free medium. Supplementary serum is still required.