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        【求助】关于caspase-3的活化

        丁香园论坛

        3973
        最近想做caspase-3的WB,但关于caspase-3的活化有一个问题一直没弄明白,想请教。

        在没受刺激前,细胞内的caspase-3以procaspase的形式存在,当受到内外源性刺激时,被活化成异二聚体,该异二聚体由p12和p17两个亚基组成。但procaspase是怎样变成异二聚体的呢?是self-cleavage,还是上游信号如caspase-8、9裂解的?
        Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

        S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol
        selenium wrote:
        Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

        S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol

        谢谢你的回答!
        但不知上段话引自何处呢?
        xdb86 wrote:
        google一下就知道了。
        第一段话引自:
        http://www.millipore.com/catalogue/item/ab1899#
        第一段话引自:
        http://microbiology.ucsf.edu/micro/faculty/liu/Mannick_Science_284.pdf


        yahoo一下也能知道 :D

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        【求助】关于caspase-3的活化

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