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文献和实验-bound and secreted forms of chicken IgM. This property allows a rapid screening and selection of antibody-producing B cells from the library by using magnetic beads conjugated with any antigen of interest. To apply the ADLib system to the direct
and is accomplished using the antigen of interest conjugated to magnetic beads. This method should significantly increase the frequency of antibody phage particles of interest in the library and allow for efficient isolation monoclonal antibodies with the predefined
). They were isolated by gradient density centrifugation using Lymphoprep (Nycomed, Oslo, Norway). CD19+ B lymphocytes were purified by positive selection using anti-CD19-conjugated magnetic beads and a VarioMacs magnetic cell sorter (Miltenyi Biotec, Bergisch Gladbach
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