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- 2025年08月12日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
Native Chromatin Preparation and Illumina/Solexa Library Construction
Immunoprecipitation (ChIP) Do NOT block beads with any nonspecific DNA or RNA. 30. Place 40 µL of Dynabeads Protein A into each of two microcentrifuge tubes. Wash each with 600 µL of 1X PBS. Place the tubes in a magnetic stand and aspirate
Isolation and Purification Method of Mouse Fetal Hepatoblasts
of magnetic beads and specific antibodies. This protocol allows the isolation of bipotent liver stem cells expressing both hepatic and biliary markers. Primary cultures of purified MFHs can be maintained under proliferation until confluence, leading
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