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文献和实验active form of human recombinant PON-1 in the mammalian cell line CHO. METHODS Construction of the paraoxonase : cloning vector The coding region for human paraoxonase-1 was amplified from human liver cDNA using a set of gene specific
, was purified in three IEC steps: a Q Sepharose Fast Flow (FF) capture step followed by a negative SP Sepharose FF step, and finally, Q Sepharose FF as a polishing step. The purification process resulted in greater than 90% pure rBoNTC(Hc )-N based on SDS-PAGE
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
1 cells are used to generate T-lineage cells, and OP9 cells are used to generate B-lineage and myelo-erythroid cells. OP9 medium Phosphate-buffered saline (PBS; Hyclone SH30256) Recombinant human Flt-3/Flk-2 ligand (R&D Systems 308-FK
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