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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 英文名:
Add&Read® Human IL2 Quantitative Detection Kit
- 供应商:
南京诺唯赞生物科技股份有限公司
- 保存条件:
-30 ~ -15℃储存,运输条件:≤0℃。
- 规格:
96 tests/500 tests/10000 tests
产品描述
白介素2(IL2)是一种主要由活化T细胞产生的免疫调节细胞因子,在体内主要起到促进T淋巴细胞和NK细胞的增殖,促进B细胞分化增殖,促进抗体生成等作用,在机体免疫应答、免疫调节和抗肿瘤免疫中具有重要作用。
本试剂盒采用夹心法检测IL2含量,试剂盒中有IL2 Standard以及两株特异性识别IL2的单克隆抗体,其中一株抗体偶联Eu(供体,Anti-IL2-Eu),另一株抗体偶联A3(受体,Anti-IL2-A3)。当两株抗体同时与IL2结合时,Anti-IL2-Eu和Anti-IL2-A3距离靠近,可发生荧光共振能量转移(FRET)。使用320/340 nm的激发光激发荧光供体,供体发射620 nm的光,此620 nm的光激发荧光受体,受体发射出665 nm的光。样本中待测IL2浓度与FRET信号值(665 nm/620 nm的光强度比值)成正比。
产品特点
均相检测:免洗ELISA,无需包被、洗板,直接加样-孵育-上机检测,节省人力、时间
节约样本:总体系仅20μl
体系稳定:24h内重复检测,信号值基本不变
高通量筛选:可搭载384孔板进行高通量筛选
检测原理

产品组分

储存条件
试剂盒于-30 ~ -15℃保存,≤0℃运输。
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